Effects of Dietary Xylo-Oligosaccharides on Growth Performance, Intestinal Digestive Enzyme Activities and Immunity of Juvenile Sea Cucumber (Apostichopus japonicus Selenka)

LI Junhua; LIU Jialiang; CAO Xuebin; LI Jing; LIU Shilin

doi:10.3969/j.issn.1006-267x.2016.08.025

2016 , Vol. 28 >Issue 8: 2534 - 2541

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.08.025

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Effects of Dietary Xylo-Oligosaccharides on Growth Performance, Intestinal Digestive Enzyme Activities and Immunity of Juvenile Sea Cucumber (Apostichopus japonicus Selenka)

LI Junhua ,
LIU Jialiang ,
CAO Xuebin ,
LI Jing ,
LIU Shilin

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1. National Alage and Sea Cucumber Project Technology Research Centre, Shandong Oriental Ocean Sci-Tech Co., Ltd., Yantai 264003, China;
2. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China

Received date: 2016-02-01

Online published: 2016-08-17

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Abstract

A feeding trial was conducted to investigate the effects of dietary xylo-oligosaccharides (XOS) on growth performance, intestinal digestive enzyme activities and immunity of sea cucumber (Apostichopus japonicus Selenka). Juvenile sea cucumbers with an initial body weight of (6.80±1.05) g were fed 6 different experimental diets containing XOS at a dose of 0 (control), 0.015%, 0.030%, 0.045%, 0.060% and 0.075%, respectively, and placed into 120 L blue plastic tanks at water temperature of 14.8 to 18.6℃ and a salinity of (29±1) PSU for 75 days. There were 3 replicates per group with 45 sea cucumbers. The results showed that XOS supplementation significantly enhanced the specific growth rate (SGR) of sea cucumbers (P<0.05). The relationship between XOS supplemental level (X) and SGR (Y) was expressed as Y=-151.68X²+13.302X+0.860 7 (R²=0.927 5), therefore there was the maximal SGR in the sea cucumbers fed the diet containing 0.044% of XOS. There were no significant differences in survival rate (SR) and gutted body weight rate (GBWR) among all groups (P>0.05). XOS supplementation led to enhance apparent digestibility of dry matter for sea cucumbers, but the significant difference only found in 0.030% group and control group (P<0.05). Diet supplemented with 0.030% to 0.060% XOS significantly increased the activities of protease and amylase in intestine and superoxide dismutase (SOD) in body fluid of sea cucumbers (P<0.05). The maximal activities of protease and amylase in intestine were found in the group with the diet containing 0.045% XOS and the maximal activity of SOD in body fluid was found in the group with the diet containing 0.060% XOS. After feeding 30 days, diet supplemented with 0.030% to 0.075% XOS significantly increased the activity of alkaline phosphatase (AKP) in body fluid of sea cucumbers (P<0.05), however, the activity of AKP in body fluid was not affected by XOS supplemental level (P>0.05) except for 0.045% when feeding 60 days. The results indicate that 0.030% to 0.060% XOS supplemented into the diets can enhance the intestinal digestive enzyme activities and immunity, and promote the growth of juvenile sea cucumbers. Base on these results, the optimal dietary XOS supplemental level is 0.044%, and the time of continuous use XOS is about 2 months.

Key words： xylo-oligosaccharides; sea cucumber (Apostichopus japonicus Selenka); growth performance; apparent digestibility; digestive enzyme; immunity

Cite this article

LI Junhua , LIU Jialiang , CAO Xuebin , LI Jing , LIU Shilin . Effects of Dietary Xylo-Oligosaccharides on Growth Performance, Intestinal Digestive Enzyme Activities and Immunity of Juvenile Sea Cucumber (Apostichopus japonicus Selenka)[J]. Chinese Journal of Animal Nutrition, 2016 , 28(8) : 2534 -2541 . DOI: 10.3969/j.issn.1006-267x.2016.08.025

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