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Effects of Compound Acidifier and Probiotics on Performance, Blood Biochemical Indices, Antioxidant Indices and Salmonella Infection of Laying Hens

  • ZHOU Ling ,
  • DING Xuemei ,
  • LUO Yuheng ,
  • WANG Jianping ,
  • BAI Shiping ,
  • MENG Huaiwang ,
  • ZHANG Keying
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  • 1. Animal Nutrition Institute, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agriculture University, Ya'an 625014, China;
    2. Chongqing Unibio Biotechnology Co., Ltd., Chongqing 400711, China

Received date: 2016-02-06

  Online published: 2016-08-17

Abstract

This study evaluated the effects of compound acidifier and probiotics on performance, blood biochemical indices, antioxidant indices and Salmonella infection of laying hens. A total of 600 50-week-old healthy laying hens with similar laying rate were randomly assigned to 4 groups that included 10 pens of 15 birds each. The hens in control group were fed a basal diet, and in the other 3 experimental groups were fed basal diets supplemented with 300 g/t probiotics, 0.1% compound acidifier in the drinking water every other day, and 300 g/t probiotics+0.1% compound acidifier in the drinking water every other day. After the feeding of 16 weeks, a total of 60 hens from control group were selected to 4 treatments as the same above for a 13-day long Salmonella challenge (1.3×108 CFU) trial. The results showed as follows:no significant effects were observed on performance, hemoglobin content, the superoxide dismutase activity and total antioxidant capacity of reproductive organ by compound acidifier and probiotics (P>0.05). Dirty egg percent was significantly decreased by compound acidifier, probiotics and combination of both (P<0.05). Compared with the control group, white blood cell count in probiotics group was significantly decreased (P<0.05), and the immunoglobulin G content in probiotics group tended to be increased (P=0.061). The malondialdehyde (MDA) content in oviduct isthmus of hens in compound acidifier group and combination group was significantly decreased compared with the control group (P<0.05). The Salmonella positive ratio in oviduct of hens in compound acidifier group was significantly decreased at the 7th and 14th day (P<0.05), but that in probiotics group and combination group was not significantly different compared with control group (P>0.05). In conclusion, compound acidifier and probiotics can improve health status of laying hens through decreasing the dirty egg percent and increasing antioxidant capacity and immunity of them, thus reducing the Salmonella infection of body and eggs.

Cite this article

ZHOU Ling , DING Xuemei , LUO Yuheng , WANG Jianping , BAI Shiping , MENG Huaiwang , ZHANG Keying . Effects of Compound Acidifier and Probiotics on Performance, Blood Biochemical Indices, Antioxidant Indices and Salmonella Infection of Laying Hens[J]. Chinese Journal of Animal Nutrition, 2016 , 28(8) : 2571 -2580 . DOI: 10.3969/j.issn.1006-267x.2016.08.030

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