Effects of Dietary Nutrient Level on mRNA Relative Expression Levels of Nutrient Sensing Factors, Blood Physiochemical Indexes and Hormone Contents of Cashmere Goats

ZHANG Xia; SUN Haizhou; SANG Dan; ZHAO Cunfa; LI Shengli; YAN Cheng; LING Shuli; SHAN Dan; REN Xiaoping

doi:10.3969/j.issn.1006-267x.2016.08.034

2016 , Vol. 28 >Issue 8: 2607 - 2618

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.08.034

SHORT COMMUNICATIONS

Effects of Dietary Nutrient Level on mRNA Relative Expression Levels of Nutrient Sensing Factors, Blood Physiochemical Indexes and Hormone Contents of Cashmere Goats

ZHANG Xia ,
SUN Haizhou ,
SANG Dan ,
ZHAO Cunfa ,
LI Shengli ,
YAN Cheng ,
LING Shuli ,
SHAN Dan ,
REN Xiaoping

Expand

1. Inner Mongolia Fuyuan Farming Co., Ltd., Hohhot 010070, China;
2. Institute of Animal Nutrition and Feed, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China

Received date: 2016-02-02

Online published: 2016-08-17

Fold

Abstract

To study the effects of N-carbamylglutamic acid (NCG) supplementation and intestinal infusion of glucose on mRNA relative expression levels of nutrient sensing factors, blood physiochemical indexes and hormone contents of cashmere goats under the condition of decreasing dietary nitrogen level. Twenty seven healthy Inner Mongolia cashmere wethers with permanent rumen fistula and duodenal cannulas were used. In accordance with the principle of similar age and body weight, twenty seven goats were divided into nine groups with three goats per group. There were 3 dietary treatments, which were low nitrogen[crude protein (CP) 10.5%], low nitrogen +NCG (0.20 g/d) and high nitrogen (CP 13.5%); meanwhile, goats in each treatment were infused glucose at 3 levels, which were 0, 20 and 40g/d. After feeding experiment (15 d of pre-experiment and 15 d of formal experiment), goats were slaughtered to collect jejunum and duodenum tissues, the mRNA relative expression levels of intestinal nutrient sensing factor were determined by real-time PCR method, and blood physiochemical indexes, and serum and jejunal hormone contents were determined. The results showed as follows:1) under the condition of basal diet (without glucose infusion), with the decrease of dietary nitrogen level, the mRNA relative expression level of sodium-glucose cotransporter 1 (SGLT1) in jejunum and duodenum, plasma urea nitrogen (UN) and glucose contents, serum citrulline and insulin contents, and serum and jejunal contents of glucagon-likepeptide 1 (GLP-1), glucagon-likepeptide 2 (GLP-2) and glucose insulinotropic peptide (GIP) were declined, but the mRNA relative expression levels of solute carrier family 7 member 9 (SLC7A9) and solute carrier family 7 member 1 (SLC7A1) were increased. 2) After increase proper amount glucose, with the decease of dietary nitrogen level, the mRNA relative expression levels of SGLT1, taste 1 receptor member 1 (T1R1), taste 1 receptor member 2 (T1R2) and taste 1 receptor member 3 (T1R3) tended to be increased. 3) Under the condition of low dietary nitrogen level and 20 g/d glucose infusion, extra supplementation of NCG could help relieve the decrease of the mRNA relative expression level of SGLT1 in jejunum and duodenum, plasma UN and glucose contents, serum citrulline content, and serum and jejunal contents of GLP-1, GLP-2 and GIP induced by the decreasing dietary nitrogen level. The results indicate that under the condition of decreasing dietary nitrogen level at proper extent, NCG supplementation and increasing rumen-passed glucose (duodenal infusion 20 g/d glucose) can improve metabolism and intestinal nutrient sensing of cashmere goats.

Key words： goat; intestinal nutrient sensing factor; glucose; N-carbamylglutamic acid

Cite this article

ZHANG Xia , SUN Haizhou , SANG Dan , ZHAO Cunfa , LI Shengli , YAN Cheng , LING Shuli , SHAN Dan , REN Xiaoping . Effects of Dietary Nutrient Level on mRNA Relative Expression Levels of Nutrient Sensing Factors, Blood Physiochemical Indexes and Hormone Contents of Cashmere Goats[J]. Chinese Journal of Animal Nutrition, 2016 , 28(8) : 2607 -2618 . DOI: 10.3969/j.issn.1006-267x.2016.08.034

References

[1] RAYBOULD H E.Gut chemosensing:interactions between gut endocrine cells and visceral afferents[J].Autonomic Neuroscience,2010,153(1/2):41-46.
[2] MCINTYRE N,HOLDSWORTH C D,LEEDS M B,et al.New interpretation of oral glucose tolerance[J].The Lancet,1964,284(7349):20-21.
[3] LAM C K L,CHARI M,LAM T K T.CNS regulation of glucose homeostasis[J].Physiology,2009,24(3):159-170.

[4] LIU Y,IPHARRAGUERRE I R,PETTIGREW J E.Digestive physiology of the pig symposium:potential applications of knowledge of gut chemosensing in pig production[J].Journal of Animal Science,2013,91(5):1982-1990.

[5] HUNTINGTON G B.Starch utilization by ruminants:from basics to the bunk[J].Journal of Animal Science,1997,75(3):852-867.
[6] HARMON D L,MCLEOD K R.Glucose uptake and regulation by intestinal tissues:implications and whole-body energetics[J].Journal of Animal Science,2001,79(E-Suppl):E59-E72.
[7] KELLETT G L,BROT-LAROCHE E,MACE O J,et al.Sugar absorption in the intestine:the role of GLUT2[J].Annual Review of Nutrition,2008,28(1):35-54.

[8] WU S V,ROZENGURT N,YANG M, et al.Expression of bitter taste receptors of the T2R family in the gastrointestinal tract and enteroendocrine STC-1 cells[J].Proceedings of the National Academy of Sciences of the United States of America,2002,99(4):2392-2397.

[9] ROZENGURT E,STERNINI C.Taste receptor signaling in the mammalian gut[J].Current Opinion in Pharmacology,2007,7(6):557-562.

[10] YOUNG R L.Sensing via intestinal sweet taste pathways[J].Frontiers in Neuroscience,2011,5:23.
[11] BURANT C F,TAKEDA J,BROT-LAROCHE E,et al.Fructose transporter in human spermatozoa and small intestine is GLUT5[J].The Journal of Biological Chemistry,1992,267(21):14523-14526.
[12] ZHAO G Q,ZHANG Y F,HOON M A,et al.The receptors for mammalian sweet and umami taste[J].Cell,2003,115(3):255-266.

[13] 赵宏丽.精氨酸对细毛羊肠道蛋白质合成、氨基酸转运蛋白mRNA表达及肠道健康的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2012.
[14] 李金霞.精氨酸及大豆油对细毛羊骨骼肌蛋白质合成和肌内脂肪含量变化的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2011.
[15] 孙海洲.生长肥育羊葡萄糖营养整体优化规律的研究[D].博士学位论文.呼和浩特:内蒙古农业大学,1999.
[16] 韩飞.反刍动物常用饲料丙酸产量和吸收率的测定及其模型的研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2001.
[17] 任莹.反刍动物常用饲料过瘤胃淀粉量及其小肠消化率测定及相关技术的研究[D].硕士学位论文.南宁:广西大学,2001.
[18] 王玲.内蒙古白绒山羊适宜代谢葡萄糖水平的评定[D].硕士学位论文.呼和浩特:内蒙古农业大学,2003.
[19] NRC.Nutrient requirements of dairy cattle[S].7th ed.Washington,D.C.:National Academy of Sciences,2001.
[20] ALDERMAN J.Energy and protein requirements of ruminants:an advisory manual prepared by the AFRC technical committee on responses to nutrients[M].Wallingford:CABI Publishing,1993.
[21] 李玫.美国Feedstuffs饲料成分分析表(2007版)[J].饲料广角,2007(12):37-40.
[22] KOKRASHVILI Z,RODRIGUEZ D,YEVSHAYEVA V,et al.Release of endogenous opioids from duodenal enteroendocrine cells requires Trpm5[J].Gastroenterology,2009,137(2):598-606.

[23] MARGOLSKEE R F,DYER J,KOKRASHVILI Z,et al.T1R3 and gustducin in gut sense sugars to regulate expression of Na⁺-glucose cotransporter 1[J].Proceedings of the National Academy of Sciences of the United States of America,2007,104(38):15075-15080.

[24] KELLETT G L.The facilitated component of intestinal glucose absorption[J].The Journal of Physiology,2001,531(3):585-595.

[25] KARASOV W H,SOLBERG D H,CHANG S D, et al.Is intestinal transport of sugars and amino acids subject to critical-period programming?[J].American Journal of Physiology-Gastrointestinal and Liver Physiology,1985,249(6):G770-G785.
[26] KARASOV W H,SOLBERG D H,DIAMOND J M.Dependence of intestinal amino acid uptake on dietary protein or amino acid levels[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,1987,252(5):G614-G625.
[27] WU G Y,KNABE D A,KIM S W.Arginine nutrition in neonatal pigs[J].The Journal of Nutrition,2004,134(10S):2783S-2390S.
[28] COLMENERO J J O,BRODERICK G A.Effect of dietary crude protein concentration on milk production and nitrogen utilization in lactating dairy cows[J].Journal of Dairy Science,2006,89(5):1704-1712.

[29] MUSCHER-BANSE A S,PIECHOTTA M,SCHRÖDER B,et al.Modulation of intestinal glucose transport in response to reduced nitrogen supply in young goats[J].Journal of Animal Science,2013,90(13):4995-5004.
[30] MARKANTONATOS X,GREEN M H,VARGA G A.Use of compartmental analysis to study ruminal volatile fatty acid metabolism under steady state conditions in Holstein heifers[J].Animal Feed Science and Technology,2008,143(1/2/3/4):70-88.
[31] KORITZ S B,COHEN P P.The effect of diet on citrulline synthesis in vitro[J].The Journal of Biological Chemistry,1953,200(2):551-557.
[32] MCGIVAN J D,BRADFORD N M,MENDES-MOURÃO J.The regulation of carbamoyl phosphate synthase activity in rat liver mitochondria[J].Biochemical Journal,1976,154(2):415-421.

[33] LARIVIÈRE F,CHIASSON J L,SCHIFFRIN A,et al.Effects of dietary protein restriction on glucose and insulin metabolism in normal and diabetic humans[J].Metabolism,1994,43(4):462-467.

[34] MACE O J,AFFLECK J,PATEL N,et al.Sweet taste receptors in rat small intestine stimulate glucose absorption through apical GLUT2[J].The Journal of Physiology,2007,582(1):379-392.

[35] JANG H J,KOKRASHVILI Z,THEODORAKIS M J,et al.Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1[J].Proceedings of the National Academy of Sciences of the United States of America,2007,104(38):15069-15074.

[36] 谭碧娥,印遇龙.胃肠营养化学感应及其生理效应[J].动物营养学报,2013,25(2):231-241.
[37] SUTHERLAND K,YOUNG R L,COOPER N J,et al.Phenotypic characterization of taste cells of the mouse small intestine[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,2007,292(5):G1420-G1428.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References