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Effects of Supplemental Feeding Letrozole on Athletic Performance, Plasma Antioxidant Indices and Hormone Levels, and Body Weight of Trotters

  • MA Jun ,
  • LI Xiaobin ,
  • DENG Haifeng ,
  • ZHAO Fang ,
  • YANG Kailun
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  • Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China

Received date: 2016-03-09

  Online published: 2016-08-17

Abstract

This experiment aimed to study the effects of supplemental feeding letrozole on athletic performance, plasma antioxidant indices and hormone levels, and body weight of trotters. Ten health Yili male horse with 2.5 years old and the average body weight of (398.16±25.34) kg, and similar performance based on strict training were randomly divided into 2 groups (control group and trial group), and each group had 5 horse. Each horse was fed with 10 kg mixed pasture and 3 kg pelleted concentrate every day, based on this, each horse of trail group was fed with 5 mg/d letrozole, and 30 d supplemental feeding experiment and training experiment were conducted. At days 0, 10, 20 and 30 of the experiment, blood samples were collected in the morning from jugular vein to determine the levels of hormones. Body weight was recorded at days 0 and 30 before ingestion in the morning. The 1 000 m trot training race was carried at days 0 and 30, and blood samples were collected from jugular vein at 1 h before the race, the end of the race, 20 min after the race and 2 h after the race to determine the plasma antioxidant indices and blood acid-base degree related indices. The results showed as follows:supplemental feeding letrozole could increase the performance of 1 000 m trot training race of trotters. The enzyme activities of catalase (CAT), glutathion peroxidase (GSH-Px), superoxide dismutase (SOD), the total antioxidant capacity (T-AOC) and the content of uric acid (UA) in plasma in trial group were higher than those in control group at 2 h after the race, and the activity of GSH-Px and T-AOC reached extremely significant (P<0.01) and significant level (P<0.05), respectively, while the malondialdehyde (MDA) in plasma in trial group was significantly lower than that in control group(P<0.05). In aspect of blood acid-base degree related indices, the content of HCO-3 in blood in trial group was lower than that in control group (P>0.05), while the BEecf and pH in blood in trial group were higher than those in control group (P>0.05) at the end of the race, 20 min after the race and 2 h after the race. The levels of testosterone (T) and androstenediol (AD) in plasma at days 10, 20 and 30 of the experiment in trial group were higher than those in control group (P>0.05). In addition, supplemental feeding letrozole could increase the body weight. Therefore, supplemental feeding 5 mg/(horse·d) letrozole for trotters can shorten the using time of the 1 000 m trot training race, increase the antioxidant ability, the acid buffering capacity, the levels of T and AD in plasma and the body weight.

Cite this article

MA Jun , LI Xiaobin , DENG Haifeng , ZHAO Fang , YANG Kailun . Effects of Supplemental Feeding Letrozole on Athletic Performance, Plasma Antioxidant Indices and Hormone Levels, and Body Weight of Trotters[J]. Chinese Journal of Animal Nutrition, 2016 , 28(8) : 2630 -2638 . DOI: 10.3969/j.issn.1006-267x.2016.08.036

References

[1] PROUS J,GRAUL J.Letrozole[J].Drugs of the Future,1994,19(4):335-337.  
[2] PAUL E G,JAMES N I,SLIAVANA R,et al.A randomized trial of letrozole in postmenopausal women after five years of tamoxifen therapy for early-stage breast cancer[J].The New England Journal of Medicine,2003,349(19):1793-1802.  
[3] WEIL S J,VENDOLA K,ZOU J,et al.Androgen and follicle-stimu-lating hormone interactions in primate follicle development[J].The Journal of Clinical Endocrinology and Metabolism,1999,83(7):2951-2956.
[4] MICHEL G,BAULIEU E.Androgen receptor in rat skeletal muscle:characterization and physiological variations[J].Endocrinology,1980,107(6):2088-2098.  
[5] 白双勇,王剑松,赵庆华.重组人生长激素联合来曲唑治疗肥胖男性少弱精子症疗效分析[J].中华男科学杂志,2015,03:280-282.
[6] 赵华,曾凡星.雄性素对运动骨骼肌MAPK和mTOR信号的作用研究[J].北京体育大学学报,2012,35(11):46-53.
[7] 刘艳丽.来曲唑法和脱氢表雄酮法诱导PCOS大鼠模型的比较研究[D].硕士学位论文.郑州:郑州大学,2011.
[8] RICHARDSON A B,JOBE F W,COLLINS H R.The shoulder in competitive swimming[J].American Journal of Sports Medicine,1980,8(3):159-163.  
[9] 王宁,王建文,孟军,等.间歇训练法对速步马血液生化指标影响的研究[J].新疆农业科学,2014,51(12):2308-2314.
[10] 姜红润,王留东.决定和影响肌肉力量的因素[J].河北体育学院学报,2003,17(3):54-57.
[11] GRIGGS R C,KINGSTON W,JOZEFOWICZ R F,et al.Effect of testosterone on muscle mass and muscle protein synthesis[J].Journal of Applied Physiology,1989,66(1):498-503.
[12] 涂晓贤.不同剂量来曲唑促排卵效果的系统评价[D].硕士学位论文.福州:福建医科大学,2013.
[13] HOLZER H,CASPER R,TULANDI A.A new era in ovulation induction[J].Fertility and Sterility,2006,85(2):277-784.  
[14] SOUZA-SILVA A A,MOREIRA E,DE M D,et al. High intensity interval training in the heat enhances exercise-induced lipid peroxidation,but prevents protein oxidation in physically active men[J].Temperature,2015,3(1)167-175.
[15] 李霖.雄激素受体在波动性高糖致人脐静脉内皮细胞损伤中的作用及其机制研究[D].硕士学位论文.杭州:浙江大学,2012.
[16] 任国庆,孙广辉.睾酮对实验兔血脂和抗氧化作用的影响[J].江苏医药,2001,27(10):785.
[17] 马海田,田朝阳,邹思湘,等.脱氮表雄酮对大鼠脂类代谢和抗氧化作用的影响[J].中国应用生理学杂志,2009,25(1):95-96.
[18] KEYMEL S,KALLKA C,RASSAF T,et al.Impaired endothelial progenitor cell function predicts age-dependent carotid intimal thickening[J].Archiv Für Kreislaufforschung,2008,103(6):582-586.
[19] MARIK P E.Handbook of evidence-based critical care[M].Switzerland:Springer International Publishing,2015:329-347.
[20] 孟军,刘志安,文立,等.伊犁马1000 m速步训练赛各阶段静脉血中血气指标变化研究[J].中国畜牧兽医,2014,11:139-143.
[21] 何伟,王绵珍,王治.两种强度运动后的血气指标变化及其与血浆K+浓度和pH值的关系[J].四川大学学报:医学版,2005,36(5):747-748.
[22] ROSA S,ADELE C,ROCCO M,et al.Insulin-like growth factor-Ⅰ,regulating aromatase expression through steroidogenic factor 1,supports estrogen-dependent tumor Leydig cell proliferation.[J].Cancer Research,2007,67(17):8368-8377.  
[23] 刘芸,邢福祺,何凌云,等.芳香化酶抑制剂在卵巢低反应者超促排卵中的作用[J].南方医科大学学报,2009,03:559-561.
[24] 陶月红,经纬,沈涛.来曲唑治疗男童青春早期乳房发育症的疗效分析[J].中华临床医师杂志,2013,20:9174-9177.
[25] 王芳,杨文静,李英勇.孕期高雄激素环境对SD大鼠仔代体重及OGTT的影响[J].现代妇产科进展,2009,11:813-815.
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