This study investigated the effects of fetal intrauterine growth retardation (IUGR) during late pregnancy on extracellular matrix synthesis in fetal liver of Mongolia sheep. Eighteen healthy Mongolia sheep with synchronization of estrus and conception were allocated to three groups with 6 replicates per group and 1 sheep per replicate. Metabolizable energy (ME) level was 0.175 (R1 group), 0.330 (R2 group) and 0.670 MJ/(kg BW0.75·d) (Ad libitum group, C group), respectively. At day 140 of gestation, ewes were slaughtered to determine parenchyma cell number and extracellular matrix contents in liver of their fetuses. The results showed that liver weight (P<0.01), liver cell number (P<0.05), liver cell nucleus diameter (P<0.01), and the contents of collagen type Ⅰ(P<0.01), collagen type Ⅲ(P<0.05), collagen type Ⅳ (P<0.01), laminin (P<0.01), hyaluronic acid (P<0.05) and fibronectin (P<0.01) in fetal liver of R1 group were significantly lower than those of C group, and endothelial cell number in fetal liver was significantly higher than that of C group(P<0.05); significantly higher fetal liver weight (P<0.05) and fibronectin content (P<0.01) were founded in R2 group compared to C group, and endothelial cell number in fetal liver was significantly enhanced in R1 group compared to C group (P<0.05). The results indicate that nutrition restriction during late pregnancy of Mongolia sheep restricts growth and development of fetal liver, and a serious impact on the synthesis of extracellular matrix is found when ME level is 0.175 MJ/(kg BW0.75·d), and liver fibrosis repair reaction also occurs; only the change of fibronectin content in liver fetal is found when ME level is 0.330 MJ/(kg BW0.75·d).
HE Shan
,
LIU Yingchun
,
LI Lingyao
,
LIANG Wei
,
GAO Feng
. Effects of Fetal Intrauterine Growth Retardation during Late Pregnancy on Extracellular Matrix Synthesis in Fetal Liver of Mongolia Sheep[J]. Chinese Journal of Animal Nutrition, 2016
, 28(9)
: 2749
-2754
.
DOI: 10.3969/j.issn.1006-267x.2016.09.012
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