A feeding trial was carried out to estimate the dietary riboflavin requirement of juvenile grass carp (Ctenopharyngodon idellus) based on growth performance, hepatopancreas D-amino acid oxidase (D-AAO) and intestinal digestive enzyme activities and body composition. Six purified diets were formulated to contain 0.56, 2.32, 4.08, 5.78, 9.28 and 19.35 mg/kg riboflavin, respectively. Juvenile grass carp with an average initial body weight of (11.21±0.16) g were fed the diets for 8 weeks, and each diet had 3 replicates with 20 fish per replicate. The results showed as follows: survival rate in 0.56 and 2.32 mg/kg groups was significantly lower than that in other groups (P<0.05). Weight gain rate (WGR), specific growth rate (SGR), feed efficiency rate (FER), hepatopancreas D-AAO and intestinal digestive enzyme activities of fish were firstly increased and then seemed to stabilize with increasing dietary riboflavin content, and the biggest values of them appeared when dietary riboflavin content was 5.78 mg/kg. Hepatosomatic index (HSI) in 5.78 mg/kg group was significantly higher than that in 0.54 mg/kg group (P<0.05). Viscerasomatic index (VSI), condition factor (CF) and the contents of whole body moisture, crude protein, crude lipid and ash were not significantly affected by dietary riboflavin content (P>0.05). Broken line regression analysis shows that dietary riboflavin requirement of juvenile grass carp with the best growth and hepatopancreas D-AAO activity is 5.54 and 5.99 mg/kg, respectively.
JIANG Jianhu
,
CHEN Jianming
,
SHEN Binqian
,
PAN Qian
,
SUN Lihui
,
HUANG Aixia
. Dietary Riboflavin Requirement of Juvenile Grass Carp (Ctenopharyngodon idellus)[J]. Chinese Journal of Animal Nutrition, 2016
, 28(9)
: 2771
-2777
.
DOI: 10.3969/j.issn.1006-267x.2016.09.015
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