This study was conducted to evaluated the effects of conjugated linoleic acid (CLA) on adipogenic trans-differentiation and myogenic differentiation of C2C12 myoblasts. Either c9,t11-CLA or t10,c12-CLA (the final concentration was 50 μmol/L) was used to treat the C2C12 mouse origin myoblasts which were cultured and induced adipogenic trans-differentiation and normal myogenic differentiation, respectively. And set the control group. The C2C12 myoblasts adipogenic trans-differentiation on the tenth day and myogenic differentiation on the eighth day were used for real-time quantitative PCR detection, and to observed the effects of c9,t11-CLA and t10,c12-CLA on different differentiation of C2C12 myoblasts. The results showed as follows: 1) compared with the control group, c9,t11-CLA promoted adipogenic trans-differentiation of C2C12 myoblasts, significantly increased the intracellular triglycerides (TG) content and up-regulated intracellular genes expression levels of the fatty acid synthetase (FAS), CCAAT/enhancer binding protein α (C/EBPa), peroxisome proliferators-activated receptors γ (PPARγ) and fatty acid binding protein 4 (FABP4) (P<0.05). Compared with the control group, t10,c12-CLA inhibited the adipogenic trans-differentiation of C2C12 myoblasts, significantly decreased the intracellular TG content and down-regulated intracellular genes expression levels of C/EBPa, PPARγ and FABP4 (P<0.05). The results of Western blotting showed that protein expression levels of FAS and FABP4 also changed as same as the gene expression levels. 2) Compared with the control group, t10,c12-CLA inhibited the myogenic differentiation of C2C12 myoblasts, significantly decreased the intracellular myotube numbers/cell numbers and down-regulated intracellular genes expression levels of myogenin (MYOG) and myogenic differentiation antigen (MYOD) (P<0.05). Compared with the control group, c9,t11-CLA significantly down-regulated gene expression level of MYOG (P<0.05) and promoted the myogenic differentiation of C2C12 myoblasts to some degree. The results of Western blotting showed that protein expression levels of MYOG and MYOD also changed as same as the gene expression levels. All these results suggest that CLA has important regulation on normal myogenic differentiation and adipogenesis trans-differentiation of skeletal muscle cells in animals.
QI Renli
,
WANG Qi
,
WANG Jing
,
YANG Feiyun
,
LIU Zuohua
,
HUANG Jinxiu
. Effects of Conjugated Linoleic Acid on Adipogenic and Myogenic Differentiation of C2C12 Myoblasts[J]. Chinese Journal of Animal Nutrition, 2016
, 28(9)
: 2778
-2785
.
DOI: 10.3969/j.issn.1006-267x.2016.09.016
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