Effects of Grape Procyanidins on Growth Performance and Immune Function of Broiler Chicks Challenged with Eimeria tenella

YANG Jinyu; ZHANG Haijun; WANG Jing; YUE Hongyuan; WU Shugeng; QI Guanghai; GUAN Kun; GERRITS Gert Jan

doi:10.3969/j.issn.1006-267x.2016.09.019

2016 , Vol. 28 >Issue 9: 2802 - 2811

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.09.019

FEED SCIENCE AND TECHNOLOGY

Effects of Grape Procyanidins on Growth Performance and Immune Function of Broiler Chicks Challenged with Eimeria tenella

YANG Jinyu ,
ZHANG Haijun ,
WANG Jing ,
YUE Hongyuan ,
WU Shugeng ,
QI Guanghai ,
GUAN Kun ,
GERRITS Gert Jan

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1. Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2. Tianjin DKVE Animal Nutrition Co., Ltd., Tianjin 300457, China

Received date: 2016-03-23

Online published: 2016-09-08

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Abstract

This study was conducted to investigate the effects of dietary grape procyanidins (GPC) on the performance and immune function of broiler chicks challenged with Eimeria tenella. A total of 350 one-day-old healthy male broiler chicks (Cobb-500) were randomly allotted into 5 groups with 7 replicates per group and 10 chicks per replicate. Chicks in blank control (BC) group and negative control (NC) group were fed the basal diet, and the others in experimental groups were fed the basal diet supplemented with 7.5, 15.0 and 30.0 mg/kg GPC, respectively. At 14 days of age, chicks in NC group and 3 experimental groups were challenged with 5×10⁴ Eimeria tenella oocysts per bird by oral, whereas chicks in BC group administered by perfusion with the same amount of saline. The experimental period lasted for 42 days containing 2 periods of early period (1 to 21 days of age) and later period (22 to 42 days of age). The results showed as follows: 1) compared with BC group, the average daily gain (ADG) of broiler chicks during all experimental stages in NC group was significantly decreased and the ratio of feed to gain (F/G) during 22 to 42 and 1 to 42 days of age was significantly increased (P<0.05). Dietary supplemented with 7.5 and 15.0 mg/kg GPC significantly inhibited the ADG decrease and F/G increase induced by Eimeria tenella challenged during 22 to 42 and 1 to 42 days of age (P<0.05). Dietary supplemented with 30.0 mg/kg GPC significantly decreased the ADG and average daily feed intake (ADFI) during 22 to 42 days of age and ADG during 1 to 42 days of age (P<0.05). 2) Compared with NC group, dietary supplemented with GPC significantly decreased cecal lesion score, bloody stool piles and the number of coccidial oocysts excretion (P<0.05). 3) Dietary supplemented with GPC significantly inhibited the increase of the spleen index of broiler chicks induced by Eimeria tenella challenged (P<0.05). T lymphocyte transformation rate (TLR) was significantly increased by Eimeria tenella challenged (P<0.05), and dietary supplemented with 7.5 and 15.0 mg/kg GPC further increased it (P>0.05). Dietary supplemented with 7.5 and 15.0 mg/kg GPC increased the B lymphocyte transformation rate (BLR) (P>0.05). The γδ T lymphocytes percentage in peripheral blood was significantly increased by Eimeria tenella challenged (P<0.05), and dietary supplemented with 15.0 mg/kg GPC further increased it (P>0.05). 4) Compared with NC group, the ileum intraepithelial lymphocytes (IEL) number and caecal mucosa secretory IgA (sIgA) level were significantly increased by Eimeria tenella challenged (P<0.05), and dietary supplemented with 7.5 and 15.0 mg/kg GPC further increased them (P<0.05). In conclusion, dietary supplemented with GPC improves immune function and relieve growth inhibition of broiler chicks challenged with Eimeria tenella, and the supplementation of 7.5 and 15.0 mg/kg GPC have the better anticoccidial effect.

Key words： grape procyanidins; broiler chicks; Eimeria tenella; growth performance; immune function

Cite this article

YANG Jinyu , ZHANG Haijun , WANG Jing , YUE Hongyuan , WU Shugeng , QI Guanghai , GUAN Kun , GERRITS Gert Jan . Effects of Grape Procyanidins on Growth Performance and Immune Function of Broiler Chicks Challenged with Eimeria tenella[J]. Chinese Journal of Animal Nutrition, 2016 , 28(9) : 2802 -2811 . DOI: 10.3969/j.issn.1006-267x.2016.09.019

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