This experiment was conducted to investigate the effects of dietary protein and fat levels on growth performance, nutrient digestibility and nitrogen metabolism of female minks during growing period, in order to determine the optimum dietary protein and fat levels of female minks during growing period. A 2×3 factorial design was adopted in this experiment to designed 6 kinds of experimental diets with 2 protein levels[32% (L) and 36% (H)] and 3 fat levels [10% (L), 20% (M) and 30% (H)]. In accordance with protein and fat levels, six kinds of experimental diets were named as LL, LM, LH, HL, HM and HH, respectively. A total of 90 healthy female minks at the age of 60 days were randomly assigned into 6 groups with 15 replicates per group and 1 mink per replicate. The adaptation period lasted for 7 days and the formal period lasted for 60 days. The results showed as follows: the highest values of final weight and average daily gain (ADG) were found in LH group, they were significantly higher than those in LL and HL groups (P<0.05), and they had a increasing trend with dietary fat level increasing. Average daily feed intake (ADFI) and feed/gain (F/G) were the lowest in LH group; the ADFI in 30% fat level group was extremely significantly lower than that in 20% fat level group (P<0.01); the F/G was increased with dietary fat level increasing, and that in 30% fat level group was extremely significantly lower than that in 10% and 20% fat level groups (P<0.01). Dry matter digestibility and fat digestibility in LL and HL groups were extremely significantly lower than those in other groups (P<0.01); protein digestibility in HH group was significantly higher than that in LL, LH and HL groups (P<0.05). With dietary protein level increasing, protein digestibility and fat digestibility were significantly (P<0.05) and extremely significantly (P<0.01) increased, respectively. Dry matter digestibility and fat digestibility in 10% fat level group were extremely significantly lower than those in 20% and 30% fat level groups (P<0.01); the fat digestibility showed an increasing trend with dietary fat level increasing; carbohydrate digestibility in 30% fat level group was the lowest, and extremely significantly lower than that in 10% and 20% fat level groups (P<0.01). Nitrogen intake in HM group was the highest, and extremely significantly higher than that in other groups (P<0.01); fecal nitrogen output in HL group was the highest, and significantly higher than that in LH and HH groups (P<0.05); the lowest value of urine nitrogen output was found in LH group, while the highest values of nitrogen deposition and biological value of nitrogen were found in LH group. With protein level from 32% increased to 36%, the nitrogen intake and urine nitrogen output were extremely significantly increased (P<0.01), while the biological value of nitrogen was significantly decreased (P<0.05). The nitrogen intake in 20% fat level group was the highest, and extremely significantly higher than that in 30% fat level group (P<0.01); the fecal nitrogen output and urine nitrogen output in 30% fat level group were the lowest, and had significant (P<0.05) or extremely significant (P<0.01) differences compared with 10% and 20% fat level groups; with dietary fat level increasing, the nitrogen deposition was firstly increased and then down, while the biological value of nitrogen was increased. Considering all the indices, when the dietary protein level is 32% and the fat level is 20% to 30% under the condition of this experiment, the female minks during growing period can get the best growth performance. Furthermore, suitable protein and fat levels of diets for minks can decrease urine nitrogen output and improve protein utilization.