This experiment was conducted to investigate the effects of rumen-protected methionine (RPMet) and rumen-protected lysine (RPLys) supplementations in diet on meat production performance and meat quality of Holstein bulls. Twenty five Holstein bulls aged about 12 months with similar body weight were selected and divided into 5 groups by random experiment design, and each group included 5 replicates with 1 bull per replicate. The control group was fed a basal diet (ranch practical diet), and the other 4 groups were fed the basal diet added with RPMet 15 g/d (RPM group), RPLys 30 g/d (RPL group), RPLys 30 g/d+RPMet 15 g/d (RPL+M group) and RPLys 30g/d+RPMet 15g/d (meanwhile dietary crude protein level was lowered 1.71%) [RPL+M(L) group], respectively. Adaptation and experimental periods lasted for 10 and 150 d, respectively. The results showed as follows: 1) RPL+M and RPL+M(L) groups had significantly higher finial weight, average daily gain than control group (P<0.05), and had significantly lower ratio of feed to gain than control group (P<0.05), but there were no differences between the above two groups (P>0.05); the highest economic benefit of farming was RPL+M (L) group [7.67 RMB/(d·bull)], which was 0.32 RMB/(d·bull) higher than that of RPL+M group. 2) Compared with control group, meat percentage was not affected by RPMet and RPLys supplementations (P>0.05); RPM, RPL, RPL+M and RPL+M(L) groups had significantly higher body weight before slaughter (P<0.05), and the value reached the highest in RPL+M group, but they were not significantly different from those in RPL+M(L) group (P>0.05); RPL group had significantly higher dressing percentage (P<0.05); all experimental groups had significantly higher meat percentage of carcass and ratio of meat to bone (P<0.05), and there were no significant differences among experimental groups (P>0.05). 3) RPMet and RPLys supplementations in diet had no significant effects on eye muscle area, shear force, cooked meat rate, marbling degree, pH and meat color degree (P>0.05). 4) There was no significant difference on muscle fatty acid composition (P>0.05). It is concluded that dietary supplementations of RPMet and RPLys can maintain similar meat quality and muscle fatty acid composition of Holstein bulls, and can improve meat production performance; and the supplementations of RPMet and RPLys can instead part of protein feed, and can increase the economic benefit of farming.