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Adaptation of Juvenile Epinephelus moara Fed Diets with High Energy and Low Nitrogen

  • ZHANG Tingting ,
  • CHEN Chao ,
  • SHAO Yanxiang ,
  • LI Yanlu ,
  • YU Huanhuan ,
  • PENG Shiming ,
  • YANG Chuanjun
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  • 1. College of Fisheries and Science, Shanghai Ocean University, Shanghai 201306, China;
    2. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
    3. Dalian Ocean University, Dalian 116023, China;
    4. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China;
    5. Mingbo Aquatic Co., Ltd., Laizhou 261418, China

Received date: 2016-03-16

  Online published: 2016-09-08

Abstract

A 9-week trial was carried out to assess the adaptation of juvenile Epinephelus moara fed diets with high energy and low nitrogen. Nine practical diets were formulated to contain three protein levels [35% (P35), 40%(P40) and 45%(P45)] and three lipid levels [9% (L9), 12% (L12) and 15%(L15)] by two-factor experimental design, and in turn marked P35L9, P35L12, P35L15, P40L9, P40L12, P40L15, P45L9, P45L12 and P45L15. Eight hundred and ten juvenile Epinephelus moara with the average initial body weight of 27.09 g were randomly divided in 9 groups with 3 replicates, and each replicate had 30 fish. After feeding for 9 weeks, the growth performance, muscle nutritive composition and serum biochemical parameters of juvenile Epinephelus moara were measured. The results showed as follows: 1) weight gain rate (WGR) and specific growth rate (SGR) were firstly increased and then decreased with the increase of dietary protein and lipid levels, and significant interactions between dietary protein and lipid levels were observed in WGR and SGR (P<0.05). The highest values of WGR and SGR were all found in P40L12 group which were 184.59% and 1.49%/d, respectively. Feed conversion ratio (FCR), protein efficiency ratio (PER) and feed intake (FI) reduced gradually with the increase of dietary protein and lipid levels, and significant interactions between dietary protein and lipid levels were observed in them (P<0.05). Survival rate showed no significant difference among 9 groups (P>0.05). 2) Muscle crude lipid content was significantly affected by dietary lipid level (P<0.05), and the highest value was observed in L15 group; dietary protein, lipid levels and their interaction had no significant effects on the contents of crude protein, moisture and ash of muscle (P>0.05). 3) serum glutamic oxalacetic transaminase (GOT) activity was increased with increase dietary lipid level, and the highest value (121.98 U/L) appeared in L15 group, which had significant difference compared with L9 group (P<0.05); serum glutamic pyruvic transaminase (GPT) activity was increased with increase dietary protein level, and the highest value (89.79 U/L) appeared in P45 group; serum total cholesterol content was positively correlated with dietary lipid level (P<0.05), whereas it was not significantly affected by dietary protein level (P>0.05); serum urea nitrogen (UN) content was negatively correlated with dietary protein level (P<0.05), and it had the highest value (1.97 mmol/L) in P35 group, which significantly higher than that in other protein level groups (P<0.05); dietary protein, lipid levels and their interaction had no significant differences on serum alkaline phosphatase (AKP) activity and the contents of triglyceride (TG), high-density lipoprotein (HDL-C) and low-density lipoprotein (LDL-C) (P>0.05). According to these results, juvenile Epinephelus moara fed the diet with 40% protein level and 12% lipid level has the best growth effect, and the utilization ratio of feed is higher under the condition of this experiment.

Cite this article

ZHANG Tingting , CHEN Chao , SHAO Yanxiang , LI Yanlu , YU Huanhuan , PENG Shiming , YANG Chuanjun . Adaptation of Juvenile Epinephelus moara Fed Diets with High Energy and Low Nitrogen[J]. Chinese Journal of Animal Nutrition, 2016 , 28(9) : 2977 -2986 . DOI: 10.3969/j.issn.1006-267x.2016.09.038

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