RUMINANT NUTRITION

Dietary Forage to Concentrate Ratio Affects Ruminal Bacterial and Methanogen Community Composition of Water Buffaloes

  • LIN Bo ,
  • LIANG Xin ,
  • LI Lili ,
  • WEI Shengju ,
  • LI Ping ,
  • ZOU Caixia
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  • 1. College of Animal Science, Guangxi University, Nanning 530004, China;
    2. Key Laboratory of Buffalo Genetics, Breeding and Reproduction of Ministry of Agriculture, Key Laboratory of Buffalo Genetics, Breeding and Reproduction of Guangxi, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China

Received date: 2016-03-28

  Online published: 2016-10-17

Abstract

This study was conducted to study the effects of dietary forage to concentrate ratio on ruminal bacterial and methanogen community composition of water buffaloes. Fifteen healthy similar hybrid water buffaloes were selected and divided into three groups with 5 heads per group according to body weight and feed intake. The three groups were fed diets with concentrate to forage ratio at 0:100 (all forage group), 35:65 (low concentrate group) and 50:50 (medium concentrate group), respectively. The total experimental period was 40 days, including 10 days of advanced experiment feeding period. On day 1 after the experiment, rumen fluid sample was taken from rumen through mouth, and ruminal fluid DNA was extracted to research ruminal bacterial and methanogen community by Illumina Miseq 250PE. The results showed as follows:1) at phyla level, ruminal bacterial community of buffaloes was dominated by Bacteroidetes (45% to 65%) and Firmicutes (13% to 27%) and Proteinobacter (13% to 18%); compared with low concentrate group and medium concentrate group, all forage group increased the proportions of Bacteroidetes, Fibrobacter and SR1, but decreased the proportions of Firmicutes, Verrucomicrobia, Actinobacteria and Chloroflexi; at the family level, the dominant bacteria were Prevotellaceae (15% to 32%) and Fibrobacteraceae (8% to 21%); compared with medium concentrate group, all forage group and low concentrate group increased the proportions of Prevotellaceae, while decreased the proportions of Fibrobacteraceae, Lachnospiraceae, Ruminococcaceae, Coriobacteriaceae and Bifidobacteriaceae; additionally, the bacterial community in medium concentrate group was more diverse than that in all forage group. 2) Ruminal methanogen community of water buffaloes was dominated by Methanobrevibacter (over 90%), followed by Thermoplasma; dietary concentrate to forage ratio had no influence on ruminal methanogen community of water buffaloes. In conclusion, bacteria related with fiber digestion tends to be increased by all forge diet, but ruminal bacterial diversity is decreased by all forage diet; there is no influence of concentrate to forage ratio on ruminal methanogen composition of water buffaloes at genus level.

Cite this article

LIN Bo , LIANG Xin , LI Lili , WEI Shengju , LI Ping , ZOU Caixia . Dietary Forage to Concentrate Ratio Affects Ruminal Bacterial and Methanogen Community Composition of Water Buffaloes[J]. Chinese Journal of Animal Nutrition, 2016 , 28(10) : 3101 -3109 . DOI: 10.3969/j.issn.1006-267x.2016.10.012

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