Effects of Different Lipid and Glucose Levels on Growth Performance, Hepatic Glycolysis and Gluconeogenic Key Enzyme Activities of Large Yellow Croaker (Larmichthys crocea Richardson)

MA Hongna; ZHOU Piaoping; LU You; YUAN Ye; HOU Yingmei; SUN Peng; DING Liyun; ZHOU Qicun

doi:10.3969/j.issn.1006-267x.2016.10.013

2016 , Vol. 28 >Issue 10: 3110 - 3122

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.10.013

AQUACULTURE NUTRITION

Effects of Different Lipid and Glucose Levels on Growth Performance, Hepatic Glycolysis and Gluconeogenic Key Enzyme Activities of Large Yellow Croaker (Larmichthys crocea Richardson)

MA Hongna ,
ZHOU Piaoping ,
LU You ,
YUAN Ye ,
HOU Yingmei ,
SUN Peng ,
DING Liyun ,
ZHOU Qicun

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Laboratory of Fish Nutrition, School of Marine Science, Ningbo University, Ningbo 315211, China

Received date: 2016-04-05

Online published: 2016-10-17

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Abstract

An 8-week feeding trial was conducted to evaluate the effects of different lipid and glucose levels on growth performance, hepatic glycolysis and gluconeogenic key enzyme activities, serum biochemical indices, glycogen contents and digestive enzyme activities etc. of large yellow croaker. Six experimental diets were formulated to contain two lipid levels (5% and 10%) and three glucose levels (10%, 20% and 30%) using 2×3 double-factor experimental design. Each diet was randomly assigned to 3 replicates of 50 juvenile large yellow croakers with the initial body weight of (14.79±0.13) g. The results showed that weight gain rate (WGR), specific growth rate (SGR) and feed efficiency (FE) were not significantly affected by the interaction of dietary lipid and glucose levels (P>0.05). When the dietary lipid level was 5%, the WGR and SGR were decreased with the increase of dietary glucose level, which in 30% glucose group were significantly lower than those in 10% glucose group (P<0.05). The interaction of dietary lipid and glucose levels had significant effects on the contents of crude lipid and moisture of whole body (P<0.05), but had no significant effects on the content of crude protein of whole body (P>0.05). The contents of hepatic glycogen and muscle glycogen were significantly affected by the interaction of dietary lipid and glucose levels (P<0.05). When the dietary lipid level was 5%, hepatic glycogen content was increased with the dietary glucose level increasing, while the muscle glycogen content was firstly increased and then down. But when the dietary lipid level was 10%, hepatic glycogen content was firstly increased and then decreased with the dietary glucose level increasing, while the muscle glycogen content was increased. The interaction of dietary lipid and glucose levels did not have significant effects on the contents of total protein, total cholesterol, triglyceride, glucose and the activities of alanine aminotransferase and aspartate transaminase in serum of large yellow croaker (P>0.05). However, when the dietary lipid level was the same, serum glucose content had an increase trend with dietary glucose level increasing, and that in 30% glucose group was significantly higher than that in 10% glucose group (P<0.05). Hepatic glucokinase, phosphofructokinase and phosphoenolpyruvate carboxy kinase activities were significantly affected by the interaction of dietary lipid and glucose levels (P<0.05), while there were no significant effects on pyruvate kinase, glucose-6-phosphatase and fructose-1,6-bisphosphatase activities (P>0.05). When the dietary lipid level was 10%, the hepatic glucokinase and phosphofructokinase activities were increased with dietary glucose level increasing, while the hepatic phosphoenolpyruvate carboxy activity was firstly increased and then decreased. These results demonstrate that, compared with the dietary 10% lipid level, when the dietary lipid level is 5%, large yellow croaker has the ability to maintain the balance of blood glucose content by adjusting hepatic glycogen contents, glucose metabolism key enzyme activities and effectively utilizes glucose in diets with the dietary glucose level increasing. The results of present study indicate that the optimal requirements of lipid and carbohydrate for large yellow croaker in the larval stage are recommended to be 10% and 20%, respectively.

Key words： large yellow croaker (Larmichthys crocea Richardson); glucose; lipid; growth performance; glycolysis key enzymes; gluconeogenic key enzymes

Cite this article

MA Hongna , ZHOU Piaoping , LU You , YUAN Ye , HOU Yingmei , SUN Peng , DING Liyun , ZHOU Qicun . Effects of Different Lipid and Glucose Levels on Growth Performance, Hepatic Glycolysis and Gluconeogenic Key Enzyme Activities of Large Yellow Croaker (Larmichthys crocea Richardson)[J]. Chinese Journal of Animal Nutrition, 2016 , 28(10) : 3110 -3122 . DOI: 10.3969/j.issn.1006-267x.2016.10.013

References

[1] 李会涛,麦康森,艾庆辉,等.大黄鱼对几种饲料蛋白原料消化率的研究[J].水生生物学报,2007,31(3):370-376.
[2] 张帆.大黄鱼(Pseudosciaena crocea R.)脂类营养生理和饲料替代蛋白源的研究[D].硕士学位论文.青岛:中国海洋大学,2012.
[3] MOHSENI M,HASSANI M H S,POURALI F H,et al.The optimum dietary carbohydrate/lipid ratio can spare protein in growing beluga,Huso huso[J].Journal of Applied Ichthyology,2011,27(2):775-780.

[4] WATANABE T,OHTA M,KITAJIMA C,et al.Improvement of dietary value of brine shrimp Artemia saliva for fish larvae by feeding them on ω3 highly unsaturated fatty acids[J].Bulletin of the Japanese Society of Scientific Fisheries,1982,48(12):1775-1782.

[5] TAN Q,XIE S,ZHU X,et al.Effect of dietary carbohydrate-to-lipid ratios on growth and feed utilization in Chinese longsnout catfish (Leiocassis longirostris Günther)[J].Journal of Applied Ichthyology,2007,23(5):605-610.

[6] 罗毅平,谢小军.鱼类利用碳水化合物的研究进展[J].中国水产科学,2010,17(2):381-390.
[7] 黄鹤忠,丁磊,宋学宏,等.青鱼和草鱼葡萄糖耐量的比较研究[J].中国水产科学,2005,12(4):496-500.
[8] FERNÁNDEZ F,MIQUEL A G,CÓRDOBA M,et al.Effects of diets with distinct protein-to-carbohydrate ratios on nutrient digestibility,growth performance,body composition and liver intermediary enzyme activities in gilthead sea bream (Sparus aurata,L.) fingerlings[J].Journal of Experimental Marine Biology and Ecology,2007,343(1):1-10.

[9] LI X F,WANG Y,LIU W B,et al.Effects of dietary carbohydrate/lipid ratios on growth performance,body composition and glucose metabolism of fingerling blunt snout bream Megalobrama amblycephala[J].Aquaculture Nutrition,2013,19(5):701-708.

[10] LI X F,LIU W B,LU K L,et al.Dietary carbohydrate/lipid ratios affect stress,oxidative status and non-specific immune responses of fingerling blunt snout bream,Megalobrama amblycephala[J].Fish & Shellfish Immunology,2012,33(2):316-323.

[11] 戈贤平,刘波,谢骏,等.饲料中不同碳水化合物水平对翘嘴红鲌生长及血液指标和糖代谢酶的影响[J].南京农业大学学报,2007,30(3):88-93.
[12] ENES P,PANSERAT S,KAUSHIK S,et al.Nutritional regulation of hepatic glucose metabolism in fish[J].Fish Physiology and Biochemistry,2009,35(3):519-539.

[13] 聂琴,苗惠君,苗淑彦,等.不同糖源及糖水平对大菱鲆糖代谢酶活性的影响[J].水生生物学报,2013,37(3):425-433.
[14] ENES P,PANSERAT S,KAUSHIK S,et al.Effect of normal and waxy maize starch on growth,food utilization and hepatic glucose metabolism in European sea bass (Dicentrarchus labrax) juveniles[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2006,143(1):89-96.

[15] ENES P,PANSERAT S,KAUSHIK S,et al.Growth performance and metabolic utilization of diets with native and waxy maize starch by gilthead sea bream (Sparus aurata) juveniles[J].Aquaculture,2008,274(1):101-108.

[16] 窦兵帅,梁萌青,郑珂珂,等.饲料中碳水化合物水平对鲈鱼生长、生理状态参数及体组成的影响[J].渔业科学进展,2014,35(1):46-54.
[17] SUÁREZ M D,SANZ A,BAZOCO J,et al.Metabolic effects of changes in the dietary protein:carbohydrate ratio in eel (Angilla anguilla) and trout (Oncorhynchus mykiss)[J].Aquaculture International,2002,10(2):143-156.

[18] 张世亮.饲料中糖结构、糖水平及糖脂比对瓦氏黄颡鱼幼鱼生长及糖代谢的影响[D].硕士学位论文.青岛:中国海洋大学,2011.
[19] 周华.饲料碳水化合物水平对鳡幼鱼生长、体成分及糖代谢酶活性的影响[D].硕士学位论文.武汉:华中农业大学,2011.
[20] 林淑琴.不同生长阶段大黄鱼的蛋白质和蛋/能比营养研究[D].硕士学位论文.青岛:中国海洋大学,2013.
[21] 何志刚.大黄鱼(Pseudosciaena crocea R.)和鲈鱼(Lateolabrax japonicus)苏氨酸和苯丙氨酸营养生理研究[D].硕士学位论文.青岛:中国海洋大学,2008.
[22] 申屠基康.大黄鱼对21种饲料原料表观消化率及色氨酸营养需要研究[D].硕士学位论文.青岛:中国海洋大学,2010.
[23] 林利民,王秋荣,王志勇,等.不同家系大黄鱼肌肉营养成分的比较[J].中国水产科学,2006,13(2):286-291.
[24] 周飘苹,金敏,吴文俊,等.不同养殖模式、投喂不同饵料及不同品系大黄鱼营养成分比较[J].动物营养学报,2014,26(4):969-980.
[25] MAI K S,WAN J L,AI Q H,et al.Dietary methionine requirement of large yellow croaker,Pseudosciaena crocea R[J].Aquaculture,2006,253(1/2/3/4):564-572.
[26] AOAC.Official methods of analysis[S].16th ed.Arlington:AOAC International,1995.
[27] HASSID W Z,ABRAHAM S.Chemical procedures for analysis of polysaccharides[J].Methods in Enzymology,1957,3:34-50.
[28] 王庆萍,方春林.鱼类脂肪需求研究概述[J].江西水产科技,2010(4):7-9.
[29] 李爱杰.水产动物营养与饲料学[M].北京:中国农业出版社,1996:44-45.
[30] DIAS J,ALVAREZ M J,DIEZ A,et al.Regulation of hepatic lipogenesis by dietary protein/energy in juvenile European sebass (Dicentrarchus labrax)[J].Aquaculture,1998,161(1/2/3/4):169-186.
[31] NRC.Nutrient requirements of fish and shrimp[S].Washington,D.C.:National Academies Press,2011.
[32] 谭肖英,罗智,刘永坚.鱼类对饲料中糖的利用研究进展[J].中国饲料,2007(6):19-23.
[33] AL-ASGAH N A,ALI A.Feeding of various carbohydrate sources on the growth performance and nutrient utilization in "Oreochromis niloticus"[J].Agribiological Research,1994,47(1):1-12.
[34] 王菲,李向飞,李贵锋,等.不同糖脂比对建鲤幼鱼生长、体组成、消化及糖酵解能力的影响[J].水产学报,2015,39(9):1386-1394.
[35] COWEY C B,ADRON J W,BROWN D A,et al.Studies on the nutrition of marine flatfish.The metabolism of glucose by plaice (Pleuronectes platessa) and the effect of dietary energy source on protein utilization in plaice[J].British Journal of Nutrition,1975,33(2):219-231.

[36] SHIMENO S,MING D C,TAKEDA M.Metabolic response to dietary carbohydrate to lipid ratios in Oreochromis niloticus[J].Nippon Suisan Gakkaishi,1993,59(5):827-833.

[37] MOKOGINTA I,TAKEUCHI T,HADADI A,et al.Different capabilities in utilizing dietary carbohydrate by fingerling and subadult giant gouramy Osphronemus gouramy[J].Fisheries Science,2004,70(6):996-1002.

[38] ERFANULLAH E,JAFRI A K.Effect of dietary carbohydrate-to-lipid ratio on growth and body composition of walking catfish (Clarias batrachus)[J].Aquaculture,1998,161(1/2/3/4):159-168.
[39] DENG D F,REFSTIE S,HUNG S S O.Glycemic and glycosuric responses in white sturgeon (Acipenser transmontanus) after oral administration of simple and complex carbohydrates[J].Aquaculture,2001,199(1/2):107-117.
[40] 蔡春芳,陈立侨,叶元土,等.日粮糖种类和水平对青鱼生长性能和生理指标的影响[J].动物营养学报,2009,21(2):212-218.
[41] HUTCHINS C G,RAWLES S D,GATLIN Ⅲ D M.Effects of dietary carbohydrate kind and level on growth,body composition and glycemic response of juvenile sunshine bass (Morone chrysops ♀×M. saxatilis )[J].Aquaculture,1998,161(1/2/3/4):187-199.
[42] 王猛强,周飘苹,黄文文,等.不同蛋白质水平下葡萄糖添加水平对大黄鱼生长性能、糖酵解和糖异生关键酶活性的影响[J].动物营养学报,2015,27(8):2431-2442.
[43] 王猛强,黄文文,周飘苹,等.不同蛋白质和小麦淀粉水平对大黄鱼生长性能、糖酵解和糖异生关键酶活性的影响[J].水产学报,2015,39(11):1690-1701.
[44] LANARI D,POLI B M,BALLESTRAZZI R,et al.The effects of dietary fat and NFE levels on growing European sea bass (Dicentrarchus labrax L.).Growth rate,body and fillet composition,carcass traits and nutrient retention efficiency[J].Aquaculture,1999,179(1/2/3/4):351-364.
[45] ALI M Z,JAUNCEY K.Optimal dietary carbohydrate to lipid ratio in African catfish Clarias gariepinus (Burchell 1822)[J].Aquaculture International,2004,12(2):169-180.

[46] BAÑOS N,BARÓ J,CASTEJÓN C,et al.Influence of high-carbohydrate enriched diets on plasma insulin levels and insulin and IGF-Ⅰeceptors in trout[J].Regulatory Peptides,1998,77(1/2/3):55-62.
[47] LEE S M,LEE J H.Effect of dietary glucose,dextrin and starch on growth and body composition of juvenile starry flounder Platichthys stellatus[J].Fisheries Science,2004,70(1):53-58.

[48] FURUICHI M,YONE Y.Changes in activities of hepatic enzymes related to carbohydrate metabolism of fishes in glucose and insulin-glucose tolerance tests[J].Bulletin of the Japanese Society of Scientific Fisheries,1982,48(3):463-466.

[49] LIN S C,LIOU C H,SHIAU S Y.Renal threshold for urinary glucose excretion by tilapia in response to orally administered carbohydrates and injected glucose[J].Fish Physiology and Biochemistry,2000,23(2):127-132.

[50] KESHAVANATH P,MANJAPPA K,GANGADHARA B.Evaluation of carbohydrate rich diets through common carp culture in manured tanks[J].Aquaculture Nutrition,2002,8(3):169-174.

[51] GAYE-SIESSEGGER J,FOCKEN V,BECKER K.Effect of dietary protein/carbohydrate ratio on activities of hepatic enzymes involved in the amino acid metabolism of Nile tilapia,Oreochromis niloticus (L.)[J].Fish Physiology and Biochemistry,2006,32(4):275-282.

[52] 王丽娜,刘文斌,张春暖,等.饲料中非蛋白能源物质对黄颡鱼幼鱼生长及生理生化指标的影响[J].南京农业大学学报,2014,37(1):108-114.
[53] MOREIRA I S,PERES H,COUTO A,et al.Temperature and dietary carbohydrate level effects on performance and metabolic utilisation of diets in European sea bass (Dicentrarchus labrax) juveniles[J].Aquaculture,2008,274(1):153-160.

[54] HILTON J W,ATKINSON J L.Response of rainbow trout (Salmo gairdneri) to increased levels of available carbohydrate in practical trout diets[J].British Journal of Nutrition,1982,47(3):597-607.

[55] MORO G V,CAMILO R Y,MORAES G,et al.Dietary non-protein energy sources:growth,digestive enzyme activities and nutrient utilization by the catfish jundiá,Rhamdia quelen[J].Aquaculture Research,2010,41(3):394-400.

[56] DJANGMAH J S.The effects of feeding and starvation on copper in the blood and hepatopancreas,and on blood proteins of Crangon vullgaris (Fabricius)[J].Comparative Biochemistry and Physiology,1970,32(4):709-731.

[57] HEMRE G I,MOMMSEN T P,KROGDAHL Å.Carbohydrates in fish nutrition:effects on growth,glucose metabolism and hepatic enzymes[J].Aquaculture Nutrition,2002,8(3):175-194.

[58] ZHOU Q C,WANG Y L,WANG H L,et al.Dietary threonine requirements of juvenile Pacific white shrimp,Litopenaeus vannamei[J].Aquaculture,2013,392-395:142-147.
[59] 周玉,郭文场,杨振国,等.鱼类血液学指标研究的进展[J].上海水产大学学报,2001,10(2):163-165.
[60] UGOLEV A M,EGOROVA V V,KUZ'MINA V V,et al.Comparative-molecular characterization of membrane digestion in fish and mammals[J].Comparative Biochemistry and Physiology Part B:Comparative Biochemistry,1983,76(3):627-635.

[61] COWEY C B,WALTON M J.Intermediary metabolism[M]//HALVER E.Fish nutrition.New York:Academic Press,1989:259-329.
[62] CASERAS A,METÓN I,VIVES C,et al.Nutritional regulation of glucose-6-phosphatase gene expression in liver of the gilthead sea bream (Sparus aurata)[J].British journal of nutrition,2002,88(6):607-614.

[63] PANSERAT S,CAPILLA E,GUTIERREZ J,et al.Glucokinase is highly induced and glucose-6-phosphatase poorly repressed in liver of rainbow trout (Oncorhynchus mykiss) by a single meal with glucose[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2001,128(2):275-283.

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