Effects of Dietary α-Linolenic Acid Content on Growth Performance, Antioxidant Indices and Serum Biochemical Indices of Large Size Japanese Seabass (Lateolabrax japonicus)

WANG Chengqiang; XU Houguo; LIANG Mengqing; ZHENG Keke; LIU Xi

doi:10.3969/j.issn.1006-267x.2016.10.014

2016 , Vol. 28 >Issue 10: 3123 - 3133

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.10.014

AQUACULTURE NUTRITION

Effects of Dietary α-Linolenic Acid Content on Growth Performance, Antioxidant Indices and Serum Biochemical Indices of Large Size Japanese Seabass (Lateolabrax japonicus)

WANG Chengqiang ,
XU Houguo ,
LIANG Mengqing ,
ZHENG Keke ,
LIU Xi

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1. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
2. College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China

Received date: 2016-04-11

Online published: 2016-10-17

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Abstract

A 12-week feeding experiment was conducted to evaluate the effects of dietary α-linolenic acid (ALA) content on growth performance, antioxidant indices and serum biochemical indices of large size Japanese seabass (Lateolabrax japonicus), in order to get the optimal dietary ALA content for large size Japanese seabass. Six isonitrogenous and isoenergetic diets were formulated with dietary ALA content of 0.06%, 0.99%, 2.03%, 3.18%, 4.12% and 5.08% dry weight by adding perilla oil in a basal diet, respectively. Japanese seabass with the initial body weight of (207.77±0.64) g were fed experimental diets, and each diet had 3 replicates with 20 fish in each replicate. The results showed as follows:1) the specific growth rate (SGR) and feed efficiency (FE) increased at first and then decreased with the increase of dietary ALA content, and reached their peaks at the 2.03% ALA group, but no significant differences were found in SGR and FE between 2.03% and 3.18% ALA groups (P>0.05). The highest values of hepatosomatic index (HSI) and viscerasomatic index (VSI) were found in 5.08% ALA group, and significantly higher than those in 0.06% ALA group (P<0.05). 2) The body ether extract content increased with increase of dietary ALA content, while the body crude protein content increased at first and then decreased with the increase of dietary ALA content. The body crude protein content in 4.12% and 5.08% ALA groups was significantly lower than that in 0.06% ALA group (P<0.05), while the body ether extract content in 4.12% and 5.08% ALA groups was significantly higher than that in 0.06%, 0.99%, 2.03% and 3.18% ALA groups (P<0.05). No significant differences were found in body moisture and ash contents among groups (P>0.05). 3) The activity of superoxide dismutase (SOD) in serum and liver in 2.03% ALA group had no significant difference compared with 3.18% ALA group (P>0.05), but significantly higher than that in 0.06% and 5.08% ALA groups (P<0.05). The malondialdehyde (MDA) content in serum in 2.03% and 3.18% ALA groups was significantly lower than that in 0.06% and 5.08% ALA groups (P<0.05), and the MDA content in liver in 2.03% ALA group was the lowest, which significantly lower than that in other groups except 3.18% ALA group (P<0.05). 4) The activities of glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) in serum in 2.03% ALA group was the lowest, which significantly lower than those in 0.06% and 5.08% ALA groups (P<0.05). The contents of triglycerides (TG) and cholesterol (CHOL) in serum reached their peaks in 4.12% ALA group (P<0.05), and significantly higher than those in 0.06% ALA group (P<0.05). The high density lipoprotein cholesterol (HDL-C) content in serum firstly increased and then showed a declining tendency with the increase of dietary ALA content, and that in 2.03%, 3.18% and 4.12% ALA groups was significantly higher than that in other groups (P<0.05). These results indicate that optimal dietary ALA content can improve the growth of large size Japanese seabass, and increase the antioxidant ability and liver health level. The broken-line model analysis based on SGR and FE as evaluation indices indicate that the optimal dietary ALA content is 2.53% and 2.72% dry weight of diet for Japanese seabass (body weight:207.77 to 406.94 g), respectively.

Key words： large size Japanese seabass (Lateolabrax japonicus); α-linolenic acid; growth performance; serum biochemical indices; antioxidant ability

Cite this article

WANG Chengqiang , XU Houguo , LIANG Mengqing , ZHENG Keke , LIU Xi . Effects of Dietary α-Linolenic Acid Content on Growth Performance, Antioxidant Indices and Serum Biochemical Indices of Large Size Japanese Seabass (Lateolabrax japonicus)[J]. Chinese Journal of Animal Nutrition, 2016 , 28(10) : 3123 -3133 . DOI: 10.3969/j.issn.1006-267x.2016.10.014

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