Optimal Dietary Vitamin C Content for Juvenile Spotted Halibut (Verasper variegates)

WANG Zhenjie; CHEN Siqing; CHANG Qing; LIU Changlin; HU Jiancheng; YAN Junli; LU Bin

doi:10.3969/j.issn.1006-267x.2016.10.015

Chinese Journal of Animal Nutrition >

2016 , Vol. 28 >Issue 10: 3134 - 3144

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.10.015

AQUACULTURE NUTRITION

Optimal Dietary Vitamin C Content for Juvenile Spotted Halibut (Verasper variegates)

WANG Zhenjie ,
CHEN Siqing ,
CHANG Qing ,
LIU Changlin ,
HU Jiancheng ,
YAN Junli ,
LU Bin

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1. College of Fishers and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;
2. Key Laboratory for Sustainable Utilization of Marine Fisheries Resource, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China

Received date: 2016-04-24

Online published: 2016-10-17

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Abstract

This experiment was conducted to study the effects of dietary vitamin C content on growth and the functions of digestion, absorption, antioxidant and immune of juvenile spotted halibut (Verasper variegates), in order to get the optimal dietary vitamin C content for juvenile spotted halibut. Water temperature was maintained at (12.5±1.5)℃ in this experiment. Juvenile spotted halibut with the body weight of (38.0±0.8) g were randomly assigned into 7 groups with 3 replicates per group and 30 fish per replicate, and fed 7 experimental diets with the vitamin C content were 10.2, 249.1, 402.8, 616.2, 769.5, 909.4 and 1 177.8 mg/kg, respectively. The experiment lasted for 8 weeks. The results showed as follows:the highest weight gain rate, feed efficiency ratio and feeding rate were found in 616.2 mg/kg vitamin C group. The weight gain rate of 616.2 mg/kg vitamin C group was significantly higher than that of 10.2 and 909.4 mg/kg vitamin C groups (P<0.05), the feed efficiency ratio of 616.2 mg/kg vitamin C group was significantly higher than that of other groups (P<0.05), and the feeding rate of 616.2 mg/kg vitamin C group was significantly higher than that of other groups except 1 177.8 mg/kg vitamin C group (P<0.05). The fish had the maximal liver and muscle vitamin C accumulation when dietary vitamin C content was 769.5 mg/kg. Serum malondialdehyde content of 616.2 and 769.5 mg/kg vitamin C groups was significantly lower than that of other groups except 402.8 mg/kg vitamin C group (P<0.05). The activities of serum catalase and lysozyme in 402.8 mg/kg vitamin C group had the highest values, which were significantly higher than those of other groups (P<0.05). Serum superoxide dismutase activity of 402.8, 616.2 and 769.4 mg/kg vitamin C groups was significantly higher than that of other groups (P<0.05). Protease, amylase and lipase activities in stomach and intestine were at a high level when dietary vitamin C content was 402.8, 616.2 or 769.4 mg/kg. Dietary vitamin C content within the scope of 249.1 to 616.2 mg/kg could promote the growth of the intestinal mucosa and increase the fold height and intestinal wall thickness. These results suggest that dietary vitamin C content within the scope of 402.8 to 616.2 mg/kg can effectively meet the need of growth, and improve the antioxidant capacity, non-specific immunity, digestion and absorption ability of juvenile spotted halibut.

Key words： juvenile spotted halibut (Verasper variegates); vitamin C; growth; immunity; antioxidant

Cite this article

WANG Zhenjie , CHEN Siqing , CHANG Qing , LIU Changlin , HU Jiancheng , YAN Junli , LU Bin . Optimal Dietary Vitamin C Content for Juvenile Spotted Halibut (Verasper variegates)[J]. Chinese Journal of Animal Nutrition, 2016 , 28(10) : 3134 -3144 . DOI: 10.3969/j.issn.1006-267x.2016.10.015

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