Establishment of Outer Root Sheath Cell Line of Goats

CUI Zhifeng; YU Huiguo; ZHANG Zhong; WANG Hui; ZENG Yongqing

doi:10.3969/j.issn.1006-267x.2016.10.024

Chinese Journal of Animal Nutrition >

2016 , Vol. 28 >Issue 10: 3207 - 3216

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.10.024

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Establishment of Outer Root Sheath Cell Line of Goats

CUI Zhifeng ,
YU Huiguo ,
ZHANG Zhong ,
WANG Hui ,
ZENG Yongqing

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1. Shandong Vocational College of Science and Technology, Weifang 261053, China;
2. College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China

Received date: 2016-04-02

Online published: 2016-10-17

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Abstract

This study was conducted to establish a pure and genetically stable outer root sheath cell (ORSC) line of goats. Dorsal skin of lambs of Jining grey goats was collected under living condition. The method of mechanical separation conjugation with enzyme digestive was conducted to obtain ORSCs. ORSCs were cultured in keratinocyte-serum free medium with epithelial growth factor (EGF), insulin-like growth factor-Ⅰ (IGF-Ⅰ), and hydrocortisone for primary culture under the conditions of 5% carbon dioxide and 37℃. After primary cells spread the culture flask, the subculture was started. The cells at passages 8 to 10 were then cultured in DMEM/F12 with EGF, IGF-Ⅰ, hydrocortisone and FBS for long-term culture. Growth characteristic and karyotype analysis were carried out using passage 40 cells. The results showed that the in vitro cultured cell population cost 51.9 h to perform doubling, the most of chromosome type was 2n=60 type, but appeared the uncompleted chromosome; the immunocytochemecal results showed that cytokeratin-19 was positively expressed in the cell line. In conclusion, the cells separated and cultured in the present study were ORSCs that differentiated from stem cells of hair follicle of goats, and a ORSC line of goats in vitro culture was successfully established.

Key words： outer root sheath cell; cell line; Jining grey goat; growth characteristic; chromosome count

Cite this article

CUI Zhifeng , YU Huiguo , ZHANG Zhong , WANG Hui , ZENG Yongqing . Establishment of Outer Root Sheath Cell Line of Goats[J]. Chinese Journal of Animal Nutrition, 2016 , 28(10) : 3207 -3216 . DOI: 10.3969/j.issn.1006-267x.2016.10.024

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