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Effects of Different Carbohydrate Types and Carbohydrate Levels on Intestinal Digestive Enzyme Activities, Intestinal and Liver Histological Structure of Songpu Mirror Carp (Cyprinus carpio specularis)

  • LI Jinnan ,
  • WANG Chang ,
  • WANG Liansheng ,
  • ZHAO Zhigang ,
  • LUO Liang ,
  • DU Xue ,
  • XU Qiyou
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  • Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China

Received date: 2016-04-06

  Online published: 2016-10-17

Abstract

This experiment was aimed to study the effects of different carbohydrate types and carbohydrate levels on intestinal digestive enzyme activities, intestinal and liver histological structure of Songpu mirror carp (Cyprinus carpio specularis). A total of 420 Songpu mirror carp with the average body weight of (8.30±0.15) g were randomly divided into 4 groups with 3 replicates and 35 fish per replicate. The fish in 4 groups were fed 4 isonitrogenous and isolipidic experimental diets with two carbohydrate types (starch and glucose) and two carbohydrate levels (25.0% and 50.0%), and the groups were named as low starch (LS) group, high starch (HS) group, low glucose (LG) group and high glucose (HG) group, respectively. The experiment lasted for 60 days. The results showed that the intestinal amylase activity of HS group was significantly higher than that of other groups (P<0.05). The intestinal protease activity of HS group was significantly higher than that of HG and LG groups (P<0.05). The intestinal lipase activity of HG and LG groups was significantly higher than that of LS group (P<0.05). The fold height of foregut of HG group was significantly lower than that of LG group (P<0.05). The fold height of midgut of HG group was significantly lower than that of LS group (P<0.05). The fold height of hindgut of HG group was significantly lower than that of other groups (P<0.05). The muscular thickness of foregut, midgut and hindgut had no significant difference among groups (P>0.05). The folds of foregut and midgut of HG group was wide and short, and the fold density of foregut and midgut was lower than that of LS and LG groups. The membranes of hepatocyte of HS and HG groups were ruptured and demonstrated vacuolation. In conclusion, dietary contained 50% starch can improve intestinal digestive enzyme activities in Songpu mirror carp, but dietary contained 50% glucose can decrease the intestine fold height, and 50% dietary carbohydrate level has a negative impact on the histological structure of liver in Songpu mirror carp.

Cite this article

LI Jinnan , WANG Chang , WANG Liansheng , ZHAO Zhigang , LUO Liang , DU Xue , XU Qiyou . Effects of Different Carbohydrate Types and Carbohydrate Levels on Intestinal Digestive Enzyme Activities, Intestinal and Liver Histological Structure of Songpu Mirror Carp (Cyprinus carpio specularis)[J]. Chinese Journal of Animal Nutrition, 2016 , 28(10) : 3217 -3224 . DOI: 10.3969/j.issn.1006-267x.2016.10.025

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