Effects of Diquat and Lipoic Acid on Intestinal Morphology and Digestive Function of Finishing Pigs

BAO Weiguang; HAO Yue; CUI Yanjun; LI Jielei; ZHANG Xiaojun; LI Gan; WANG Zhanbin; GU Xianhong

doi:10.3969/j.issn.1006-267x.2016.10.030

Chinese Journal of Animal Nutrition >

2016 , Vol. 28 >Issue 10: 3264 - 3274

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.10.030

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Effects of Diquat and Lipoic Acid on Intestinal Morphology and Digestive Function of Finishing Pigs

BAO Weiguang ,
HAO Yue ,
CUI Yanjun ,
LI Jielei ,
ZHANG Xiaojun ,
LI Gan ,
WANG Zhanbin ,
GU Xianhong

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1. State Key Laboratory of Animal Nutrition, Chinese Academy of Agricultural Sciences, Institute of Animal Sciences, Beijing 100193, China;
2. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China

Received date: 2016-04-11

Online published: 2016-10-17

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Abstract

This study was to investigate the effects of diquat and lipoic acid (LA) on intestinal morphology and digestive function of finishing pigs, in order to explore whether diquat induced stress on the LA had a role in alleviating. Twenty-four healthy finishing pigs (Yorkshire) with the body weight of (70.64±3.61) kg were randomly allocated to control, LA, diquat, LA+diquat groups with 6 replicates per group and 1 pig per replicate by a 2×2 factorial arrangement. The trial period was 29 days. LA was given all in the trial (800 mg/kg diet). Diquat challenged pigs were given a single intraperitoneal injection of diquat at the 15th days and non-challenged pigs were injected intraperitoneally with the equal physiological saline amount at the same time. All the pigs were slaughtered after the vena cava blood was sampledon the 29th day. Using kits to detected the contents of plasma and intestine oxidative damage markers and the activities of digestive enzymes in jejunum chyme. After hematoxylin and eosin (HE) staining, using image analysis software to observed intestinal morphology structure and determined villus height, crypt depth, and calculated the value of the villus height to crypt depth (V/C). Using fluorescence quantitative PCR to detected the relative expression abundance of occludin and zonula occludens protein-1 (ZO-1) mRNA in jejunum. The results showed as follows:1) diquat significantly increased the contents of malondialdehyde (MDA), protein carbonyl (PCO) and 8-hydroxy deoxyguanosine (8-OHdG) in plasma and intestine (P<0.01), significantly reduced the villus height and crypt depth in duodenum and jejunum and ileum (P<0.01), while significantly increased the V/C in duodenum and jejunum (P<0.01), and significantly increased the V/C of the ileum (P<0.05), significantly reduced the relative expression abundance of occludin and ZO-1 mRNA in jejunum (P<0.01), and significantly reduced the activities of amylase, trypsase and lipase in jejunum chyme (P<0.01). 2) There were no significant difference on the contents of MDA, PCO and 8-OHdG in plasma and intestine when dietary supplementation of LA (P>0.05), as same as the villus height, crypt depth and V/C in duodenum, jejunum and ileum, the relative expression abundance of occludin and ZO-1 mRNA in jejunum, the activities of amylase, trypsase and lipase in jejunum chyme (P>0.05). 3) Under the oxidative stress, dietary supplementation of LA significantly reduced the content of 8-OHdG in plasma (P<0.05), while significantly improved the villus height in duodenum and jejunum (P<0.05), and significantly improved crypt depth in ileum (P<0.05), significantly reduced V/C in jejunum (P<0.05) and ileum (P<0.01), significantly increased the relative expression abundance of occludin and ZO-1 mRNA in jejunum (P<0.05), significantly improved the activity of amylase and trypsase in jejunum chyme (P<0.05). In conclusion, diquat caused oxidative stress in pigs is strong, leading to serious injury of intestinal structure and weakened intestinal digestive function. Dietary supplementation of 800 mg/kg of LA can suppress the oxidative stress caused by diquat injection on intestinal injury of finishing pigs, and may improve digestive function.

Key words： diquat; lipoic acid; finishing pigs; oxidative stress; intestine

Cite this article

BAO Weiguang , HAO Yue , CUI Yanjun , LI Jielei , ZHANG Xiaojun , LI Gan , WANG Zhanbin , GU Xianhong . Effects of Diquat and Lipoic Acid on Intestinal Morphology and Digestive Function of Finishing Pigs[J]. Chinese Journal of Animal Nutrition, 2016 , 28(10) : 3264 -3274 . DOI: 10.3969/j.issn.1006-267x.2016.10.030

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