Effects of Glutamine and Its Dipeptides on Apoptosis and Apoptosis Related Gene Expressions Induced by Hydrogen Peroxide in Ruminal Epithelial Cells of Goats

HAN Qipeng; JIE Hongdong; LUO Ling; WANG Kaijun; ZHOU Chuanshe; ZHANG Peihua; KONG Zhiwei; TANG Shaoxun

doi:10.3969/j.issn.1006-267x.2016.10.034

2016 , Vol. 28 >Issue 10: 3301 - 3310

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.10.034

SHORT COMMUNICATIONS

Effects of Glutamine and Its Dipeptides on Apoptosis and Apoptosis Related Gene Expressions Induced by Hydrogen Peroxide in Ruminal Epithelial Cells of Goats

HAN Qipeng ,
JIE Hongdong ,
LUO Ling ,
WANG Kaijun ,
ZHOU Chuanshe ,
ZHANG Peihua ,
KONG Zhiwei ,
TANG Shaoxun

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1. Key Laboratory for Agro-Ecological Processes in Subtropical Region, Hunan Research Center of Livestock & Poultry Sciences, South-Central Experimental Station of Animal Nutrition and Feed Science of Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
2. Hunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China

Received date: 2016-04-10

Online published: 2016-10-17

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Abstract

This study was conducted to investigate the effects of glutamine (Gln), glycyl-glutamine (Gly-Gln) and alanyl-glutamine (Ala-Gln) on apoptosis rate and gene expressions of Bcl-2 and Bax of apoptosis cells according to establish apoptosis models for ruminal epithelial cells of goats induced by hydrogen peroxide (H₂O₂). Subculture ruminal epithelium cells of 60 day-old Xiangdong black goats were selected and cultured with different concentrations[0(control group), 100, 400 and 800 μmol/L] of H₂O₂, and flow cytometry (FCM) technique was used to detected cell apoptosis. Subculture ruminal epithelium cells were divided into 5 groups, control group and group 1 were cultured with 0 and 800 μmol/L H₂O₂, and groups 2, 3 and 4 were cultured with 800 μmol/L H₂O₂, meanwhile with 17.28 mmol/L Gly-Gln (group 2), 16.0 mmol/L Gln (group 3) and 16.0 mmol/L Ala-Gln (group 4). FCM technique was used to detected cell apoptosis, and gene expressions of Bcl-2 and Bax were detected by real-time fluorescent quantitative PCR. The results showed as follows:1) compared with control group, when the concentration of H₂O₂ reached 800 μmol/L, apoptosis rate of early apoptosis significantly increased (P<0.05); apoptosis rate of late apoptosis firstly increased and then decreased with the increasing of H₂O₂ concentration, while compared with control group, experimental groups were all significantly increased (P<0.05). 2) Compared with control group, apoptosis rate of late apoptosis in group 4 significant increased (P<0.05), and apoptosis rate of early apoptosis in experimental groups were all significantly higher than that in control group (P<0.05). 3) Compared with control group, Bcl-2/Bax in experimental groups was significantly increased (P<0.05); compared with group 1, Bcl-2/Bax in groups 2, 3 and 4 was significantly increased (P<0.05), and group 2 was significantly higher than groups 3 and 4 (P<0.05). In conclusion, Gly-Gln plays protection role in early apoptosis induced by H₂O₂ in ruminal epithelium cells of goats.

Key words： Xiangdong black goat; ruminal epithelium cell; apoptosis; apoptosis gene

Cite this article

HAN Qipeng , JIE Hongdong , LUO Ling , WANG Kaijun , ZHOU Chuanshe , ZHANG Peihua , KONG Zhiwei , TANG Shaoxun . Effects of Glutamine and Its Dipeptides on Apoptosis and Apoptosis Related Gene Expressions Induced by Hydrogen Peroxide in Ruminal Epithelial Cells of Goats[J]. Chinese Journal of Animal Nutrition, 2016 , 28(10) : 3301 -3310 . DOI: 10.3969/j.issn.1006-267x.2016.10.034

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