Effects of Subacute Ruminal Acidosis on Ruminal Epithelial Morphology and Permeability in Dairy Goats

CHENG Meng; YANG Shuqing; GAO Min; XIE Tianyu; SUN Yanyong; HU Honglian

doi:10.3969/j.issn.1006-267x.2016.10.035

Chinese Journal of Animal Nutrition >

2016 , Vol. 28 >Issue 10: 3311 - 3319

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.10.035

SHORT COMMUNICATIONS

Effects of Subacute Ruminal Acidosis on Ruminal Epithelial Morphology and Permeability in Dairy Goats

CHENG Meng ,
YANG Shuqing ,
GAO Min ,
XIE Tianyu ,
SUN Yanyong ,
HU Honglian

Expand

1. College of Animal Sciences, Inner Mongolia Agricultural University, Huhhot 010018, China;
2. Jiangsu Mengbili Biological Technology Co., Ltd., Changzhou 213100, China;
3. Institute of Animal Nutrition and Feed, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China;
4. Inner Mongolia Holy Priest High-Tech Animal Husbandry Co., Ltd., Hohhot 010018, China

Received date: 2016-04-12

Online published: 2016-10-17

Fold

Abstract

The objective of this experiment was to study the effects of subacute ruminal acidosis (SARA) on ruminal epithelial morphology and permeability in dairy goats. Nine healthy lactating Saanen dairy goats with similar body weight were randomly divided into three groups (control group, SARA group and recovery group, n=3). Dairy goats in control group were fed a basal diet[non-fibre carbohydrates/neutral detergent fibre (none fiber carbon NFC/NDF)=1.40], those in SARA group and recovery group were fed experimental diets with different NFC/NDFs (1.40, 1.79, 2.31 and 3.23 in order, each for 15 days), which gradually induced SARA, and those in recovery group were ad libitum fed green hay for 30 days after SARA. One of 3 goats in control group were slaughtered on days 30, 60 (together with 3 goats in SARA group) and 90 d of feeding (together with 3 goats in recovery group), respectively. Samples of the ruminal epithelium from ventral sac were collected to make paraffin section, observe under transmission electron microscope and do Ussing chamber analysis. The results showed as follows:1) the results of histological slice revealed that the thickness of ruminal epithelial stratum corneum in SARA group was significantly higher than that in control and recovery groups (P<0.05), and recovery group was significantly lower than control group (P<0.05); the thickness of granulosum stratum in control group was significantly higher than that in SARA and recovery groups (P<0.05), but there was no significant difference between SARA group and recovery group (P>0.05); the thickness of spinosum stratum in three groups had no significant difference (P>0.05); the total thickness of the rumen epithelium in recovery group was significantly lower than that in control and SARA group (P<0.05), but there was no significant difference between control and SARA groups (P>0.05). The results of transmission electron microscope showed rumen epithelial tight junction structure in SARA group was destroyed and intercellular space was increased,and spinosum stratum cell mitochondria was degraded and showed vacuoles. 2) Compared with control group, the short-circuit, tissue conductance and the flux rate of horseradish peroxidase of rumen epithelium in SARA and recovery groups were significantly increased (P<0.05), and potential difference was significantly decreased (P<0.05). In conclusion, SARA impairs the integrity of ruminal epithelial morphological structure in dairy goats, and causes an increase in ruminal epithelial permeability, which results in rumen epithelial barrier function long-term damage.

Key words： subacute ruminal acidosis; rumen epithelium; morphology; ultramicrostructure; permeability

Cite this article

CHENG Meng , YANG Shuqing , GAO Min , XIE Tianyu , SUN Yanyong , HU Honglian . Effects of Subacute Ruminal Acidosis on Ruminal Epithelial Morphology and Permeability in Dairy Goats[J]. Chinese Journal of Animal Nutrition, 2016 , 28(10) : 3311 -3319 . DOI: 10.3969/j.issn.1006-267x.2016.10.035

References

[1] 徐俊,苏衍菁,张养东,等.亚急性瘤胃酸中毒对瘤胃异常代谢产物和微生物组成的影响[J].中国奶牛,2012(9):17-20.
[2] 胡红莲,刘大程,卢德勋,等.日粮不同非纤维性碳水化合物与中性洗涤纤维的比值对奶山羊瘤胃液和血液中内毒素、组织胺含量的影响[J].中国畜牧兽医,2012,39(3):104-109.
[3] KHAFIPOUR E,KRAUSE D O,PLAIZIER J C.A grain-based subacute ruminal acidosis challenge causes translocation of lipopolysaccharide and triggers inflammation[J].Journal of Dairy Science,2009,92(3):1060-1070.

[4] 刘坚,侯永清,丁斌鹰,等.α-酮戊二酸对脂多糖应激断奶仔猪空肠黏膜蛋白合成和抗氧化能力的影响[J].中国畜牧杂志,2010,46(11):35-38.
[5] STEELE M A,CROOM J,KAHLER M,et al.Bovine rumen epithelium undergoes rapid structural adaptations during grain-induced subacute ruminal acidosis[J].American Journal of Physiology:Regulatory,Integrative and Comparative Physiology,2011,300(6):R1515-R1523.
[6] PENNER G B,OBA M,GÄBEL G,et al.A single mild episode of subacute ruminal acidosis does not affect ruminal barrier function in the short term[J].Journal of Dairy Science,2010,93(10):4838-4845.

[7] KLEVENHUSEN F,HOLLMANN M,PODSTATZKY-LICHTENSTEIN L,et al.Feeding barley grain-rich diets altered electrophysiological properties and permeability of the ruminal wall in a goat model[J].Journal of Dairy Science,2013,96(4):2293-2302.

[8] STEELE M A,GREENWOOD S L,CROOM J,et al.An increase in dietary non-structural carbohydrates alters the structure and metabolism of the rumen epithelium in lambs[J].Canadian Journal of Animal Science,2012,92(2):123-130.

[9] 邬宇航,殷兆丽,刘大程,等.亚急性瘤胃酸中毒对瘤胃、瓣胃上皮细胞增殖与凋亡的影响[J].动物营养学报,2013,25(11):2612-2622.
[10] 王娟.不同精粗比日粮对奶牛和山羊瘤胃上皮屏障的影响[D].硕士学位论文.南京:南京农业大学,2012.
[11] NRC.Nutrient requirements of goats:angora,dairy,and meat goats in temperate and tropical countries[S].Washington,D.C.:National Academy Press,1981.
[12] 金公亮.奶山羊饲养标准[J].畜牧兽医杂志,1989(2):7-12.
[13] RAMANZIN M,BAILONI L,SCHIAVON S,et al.Effect of monensin on milk production and efficiency of dairy cows fed two diets differing in forage to concentrate ratios[J].Journal of Dairy Science,1997,80(6):1136-1142.

[14] PENNER G,BEAUCHEMIN K A,MUTSVANGWA T.Severity of ruminal acidosis in primiparous Holstein cows during the periparturient period[J].Journal of Dairy Science,2007,90(1):365-375.

[15] 杨淑青.亚急性瘤胃酸中毒对奶山羊瘤胃上皮屏障功能影响机制的研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2014.
[16] 王彤,刘会峦.3,3',5,5'-四甲基联苯胺-H₂O₂-HRP分光光度法测定HRP的研究[J].青岛大学学报:工程技术版,2004,19(3):17-20.
[17] 张素华,李岩.瘤胃生理与奶牛过饲精料引发疾病的研究[J].动物科学与动物医学,2002,19(9):19-21.
[18] NOCEK J E,HEALD C W,POLAN C E.Influence of ration physical form and nitrogen availability on ruminal morphology of growing bull calves[J].Journal of Dairy Science,1984,67(2):334-343.

[19] LODEMANN U,MARTENS H.Effects of diet and osmotic pressure on Na⁺ transport and tissue conductance of sheep isolated rumen epithelium[J].Experimental Physiology,2006,91(3):539-550.

[20] MAEKAWA M,BEAUCHEMIN K A,CHRISTENSEN D A.Effect of concentrate level and feeding management on chewing activities,saliva production,and ruminal pH of lactating dairy cows[J].Journal of Dairy Science,2002,85(5):1165-1175.

[21] ASCHENBACH J R,GÄBEL G.Effect and absorption of histamine in sheep rumen:significance of acidotic epithelial damage[J].Journal of Animal Science,2000,78(2):464-470.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References