This experiment was conducted to investigate appropriate extract solution and solid-liquid separation method for evaluating enzyme in solid feed enzyme product. Extract solution of deionized water, acetic acid-sodium acetate buffer solution (0.1 mol/L, pH 5.50), phosphate buffer solution (0.05 mol/L, pH 6.00) or 0.9% NaCl solution and solid-liquid separation method of no separation, 3 000 r/min of centrifugation for 3 min or filtration were used in a 4×3 factorial arrangement. Each treatment contained 5 replicates with 2 determination in each. The activities of enzymes in the products were determined. Then, the effects of the types of extract solution on the contents of solute and protein were investigated for the enzyme products (excluding α-galactosidase) dissolved and centrifuged. The results showed that the highest determined activity of xylanase was presented in the phosphate buffer solution buffer solution (P<0.05). The similar determined activities of β-glucanase were observed in acetic acid-sodium acetate buffer solution, phosphate solution and 0.9% NaCl solution (P>0.05), and were significantly higher than that in deionized water (P<0.05). The greatest and greater determined activities of β-mannanase were observed in deionized water and acetic acid-sodium acetate buffer solution, respectively, and were significantly greater than those in phosphate solution or 0.9% NaCl solution (P<0.05). The type of extract solution had no significant effect on the determined activity of α-galactosidase (P>0.05). The solid-liquid separation method had no significant effect on the determined activity of xylanase (P>0.05). The greater determined activity of β-glucanase was observed in centrifugation or filtration (P<0.05). The greatest determined activity of β-mannanase was presented in centrifugation (P<0.05). However, the highest determined activity of α-galactosidase product was observed in no separation (P<0.05). There was a significant interaction between the type of extract solution and solid-liquid separation method in the determined activities of 4 enzyme products (P<0.01). The highest dry matter solubility of xylanase product was observed in acetic acid-sodium acetate buffer solution (P<0.05). The highest dry matter solubility of β-glucanase and β-mannanase was presented in deionized water and 0.9% NaCl solution (P<0.05). However, the lowest protein contents of xylanase, β-glucanase and β-mannanase were observed in acetic acid-sodium acetate buffer solution (P<0.05). It is concluded that acetic acid-sodium acetate buffer solution is the most efficient to extract the enzyme protein from the product. After dissolved, the α-galactosidase product is not suitable to separate, but the other enzymes can be separated with centrifugation.