Effects of Phytase Supplementation in a Low Zinc Diet on Growth Performance, Tibial Development, Immune Performance and Antioxidant Capacity of Geese Aged from 1 to 4 Weeks

ZHENG Huiwen; WANG Baowei; GE Wenhua; ZHANG Ming; YUE Bin; ZHANG Zenan; ZHANG Yangyang; REN Min

doi:10.3969/j.issn.1006-267x.2016.11.024

2016 , Vol. 28 >Issue 11: 3557 - 3566

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.11.024

FEED SCIENCE AND TECHNOLOGY

Effects of Phytase Supplementation in a Low Zinc Diet on Growth Performance, Tibial Development, Immune Performance and Antioxidant Capacity of Geese Aged from 1 to 4 Weeks

ZHENG Huiwen ,
WANG Baowei ,
GE Wenhua ,
ZHANG Ming ,
YUE Bin ,
ZHANG Zenan ,
ZHANG Yangyang ,
REN Min

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Nutrition and Feed Laboratory of China Agriculture Research System, Institute of High Quality Waterfowl, Qingdao Agricultural University, Qingdao 266109, China

Received date: 2016-04-25

Online published: 2016-11-18

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Abstract

This experiment was conducted to study the effect of phytase supplementation in a low zinc diet on growth performance, tibial development, immune performance and antioxidant capacity of geese aged from 1 to 4 weeks, and to explore whether phytase could effectively reduce the zinc supplemental level and increase the biological availability of zinc. A total of 360 Wulong geese were randomly divided into six groups with six replicates per group and ten geese per replicate (half male and half female). Geese in the group Ⅰ (positive control group) were fed the basal diet supplemented with 80 mg/kg zinc sulfate, and without phytase; geese in the group Ⅱ (negative control group) were fed a basal diet supplemented with 1 200 U/kg phytase, and without zinc sulfate; and the others in the groups Ⅲ to Ⅵ were fed the diets supplemented with 16, 32, 48 and 64 mg/kg zinc sulfate based on negative control group, respectively. The experiment lasted for 28 days. The results showed as follows:1) by quadratic curve fitting analysis, in the condition of dietary supplemented with 1 200 U/kg phytase, when dietary zinc supplemental level was 34.00 to 44.7 mg/kg, the average daily gain and feed to gain reached the best. 2) The bone mineral density (BMD) of groups Ⅳ and Ⅵ was significantly higher than that of group Ⅱ (P<0.05); the bone mineral content (BMC) and serum alkaline phosphatase (AKP) activity of groups Ⅳ and Ⅵ were significantly higher than those of groups Ⅰ and Ⅱ (P < 0.05 or P < 0.01). By quadratic curve fitting analysis, when the dietary zinc supplemental level was 51.38 mg/kg, the serum AKP activity reached the strongest. 3) The total antioxidant capacity (T-AOC) in serum and liver of group Ⅳ was significantly higher than that of group Ⅱ (P<0.05), and were no significant difference compared with group Ⅰ (P>0.05). By quadratic curve fitting analysis, when the zinc supplemental level was 50.24 and 47.49 mg/kg, respectively, the T-AOC in serum and liver was the highest. 4) The thymus index and bursa of Fabricius index of groups Ⅲ to Ⅵ were significantly higher than those of groups Ⅰ and Ⅱ (P < 0.05 or P < 0.01), while the level of serum antibody against avian influenza of groups Ⅳ to Ⅵ was significantly higher than that of groups Ⅰ and Ⅱ before or after immunization (P < 0.05 or P < 0.01). In conclusion, diet adding phytase can enhance the growth performance of Wulong geese aged from 1 to 4 weeks, promote development of tibia, enhance the antioxidant capacity and immune performance, improve the utilization rate of zinc, reduce the dietary zinc supplemental level, and increase the biological availability of zinc. It is suggested that the optimal zinc supplemental level is 44.70 to 51.38 mg/kg when the diet adding 1 200 U/kg phytase.

Key words： zinc; phytase; geese; growth performance; tibial development; antioxidant capacity; immune

Cite this article

ZHENG Huiwen , WANG Baowei , GE Wenhua , ZHANG Ming , YUE Bin , ZHANG Zenan , ZHANG Yangyang , REN Min . Effects of Phytase Supplementation in a Low Zinc Diet on Growth Performance, Tibial Development, Immune Performance and Antioxidant Capacity of Geese Aged from 1 to 4 Weeks[J]. Chinese Journal of Animal Nutrition, 2016 , 28(11) : 3557 -3566 . DOI: 10.3969/j.issn.1006-267x.2016.11.024

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