Effects of Glutamate on Intestinal Energy Metabolism in the Lipopolysaccharide-Challenged Weaned Piglets

QIN Qin; WANG Xiuying; WU Huanting; ZHU Huiling; LIU Yulan

doi:10.3969/j.issn.1006-267x.2016.11.031

Chinese Journal of Animal Nutrition >

2016 , Vol. 28 >Issue 11: 3618 - 3625

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.11.031

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Effects of Glutamate on Intestinal Energy Metabolism in the Lipopolysaccharide-Challenged Weaned Piglets

QIN Qin ,
WANG Xiuying ,
WU Huanting ,
ZHU Huiling ,
LIU Yulan

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Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China

Received date: 2016-05-03

Online published: 2016-11-18

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Abstract

This study was aimed to investigate the effects of glutamate (Glu) on intestinal energy metabolism in the lipopolysaccharide (LPS)-challenged weaned piglets. Twenty-four weaned pigs were assigned to four groups as control group, LPS group, LPS+1.0% Glu group and LPS+2.0% Glu group with 6 replicates each and 1 pig in per replicate. On the 28^th day of the trial, the piglets in the experimental groups were injected with 100 μg/kg BW LPS, and the piglets in the control group were injected with the same amount of 0.9% NaCl solution. At 4 h post-injection, pigs were slaughtered and intestinal samples were collected for further analysis. The results showed as follows:1) LPS challenge significantly decreased ATP and total adenine nucleotide (TAN) contents and energy charge (P<0.05), but significantly increased the ratio of AMP to ATP (P<0.05) in jejunum compared with the control group; LPS+2.0% Glu group significantly increased the contents of ATP, ADP and TAN (P<0.05) in jejunum compared with LPS group. 2) Compared with the control group, LPS challenge had a tendency to decrease the alpha-oxoglutarate dehydrogenase complex activity (P=0.092) in jejunum and extremely significantly decreased the activities of citrate synthase and alpha-oxoglutarate dehydrogenase complex (P<0.01) in ileum; compared with LPS group, Glu had no significant effects on the activities of tricarboxylic acid cycle key enzymes (P>0.05) in jejunum and ileum, except for the citrate synthase activity in ileum was significantly reduced in LPS+1.0% Glu group (P<0.05). 3) LPS challenge extremely significantly decreased the mRNA expressions of peroxisome proliferator-activated receptor gamma coactivator-1α (PGC1α) in jejunum and silent information regulator 1 (Sirt1) and PGC1α in ileum (P<0.01) compared with the control group; compared with LPS group, LPS+2.0% Glu group had a tendency to increase the mRNA expressions of PGC1α in jejunum (P=0.067) and Sirt1 in ileum (P=0.053), while LPS+1.0% Glu group had a tendency to increase the Sirt1 mRNA expression in ileum (P=0.070). These results indicate that dietary supplementation of Glu can improve energy loss status in LPS injured intestine.

Key words： glutamate; lipopolysaccharide; weaned piglets; intestine; energy metabolism

Cite this article

QIN Qin , WANG Xiuying , WU Huanting , ZHU Huiling , LIU Yulan . Effects of Glutamate on Intestinal Energy Metabolism in the Lipopolysaccharide-Challenged Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2016 , 28(11) : 3618 -3625 . DOI: 10.3969/j.issn.1006-267x.2016.11.031

References

[1] 杨凤娟,王春林,伍少钦,等.仔猪肠道健康的营养调控技术及其应用[J].中国畜牧杂志,2015,51(18):54-61.
[2] WANG Y J,LIU W,CHEN C,et al.Irradiation induced injury reduces energy metabolism in small intestine of Tibet minipigs[J].PLoS One,2013,8(3):e58970.
[3] COYNE V E.The importance of ATP in the immune system of molluscs[J].Invertebrate Survival Journal,2011,8(1):48-55.
[4] GIANNONE P J,NANKERVIS C A,RICHTER J M,et al.Prenatal lipopolysaccharide increases postnatal intestinal injury in a rat model of necrotizing enterocolitis[J].Journal of Pediatric Gastroenterology and Nutrition,2009,48(3):276-282.

[5] FAN M Z,MATTHEWS J C,ETIENNE N M P,et al.Expression of apical membrane L-glutamate transporters in neonatal porcine epithelial cells along the small intestinal crypt-villus axis[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,2004,287(2):G385-G398.
[6] WU G Y.Functional amino acids in nutrition and health[J].Amino Acids,2013,45(3):407-411.

[7] STOLL B,BURRIN D G,HENRY J,et al.Substrate oxidation by the portal drained viscera of fed piglets[J].American Journal of Physiology:Endocrinology and Metabolism,1999,277(1):E168-E175.
[8] 周顺伍.动物生物化学[M].北京:化学工业出版社,2008:121-123.
[9] WU G Y,BAZER F W,DAVIS T A,et al.Important roles for the arginine family of amino acids in swine nutrition and production[J].Livestock Science,2007,112(1/2):8-22.
[10] LIU Y L,HUANG J J,HOU Y Q,et al.Dietary arginine supplementation alleviates intestinal mucosal disruption induced by Escherichia coli lipopolysaccharide in weaned pigs[J].British Journal of Nutrition,2008,100(3):552-560.

[11] PI D A,LIU Y L,SHI H F,et al.Dietary supplementation of aspartate enhances intestinal integrity and energy status in weanling piglets after lipopolysaccharide challenge[J].The Journal of Nutritional Biochemistry,2014,25(4):456-462.

[12] 陈逢.鱼油通过TLR4和NOD信号通路对脂多糖诱导的仔猪肠道、肝脏损伤和肌肉蛋白质降解的调控作用[D].硕士学位论文.武汉:武汉轻工大学,2013.
[13] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT method[J].Methods,2001,25(4):402-408.

[14] KADENBACH B.Regulation of respiration and ATP synthesis in higher organisms:hypothesis[J].Journal of Bioenergetics and Biomembranes,1986,18(1):39-54.

[15] 付大波,王友炜,侯永清,等.α-酮戊二酸对脂多糖刺激断奶仔猪肌肉能量代谢的影响[J].中国农业科学,2011,44(4):814-822.
[16] 解雪芬,朱毅.AMPK与代谢综合征[J].基础医学与临床,2006,26(1):27-34.
[17] 王岩,石冬梅,向瑞平,等.内毒素对肉鸡肠黏膜及肝线粒体复活体Ⅰ活性的影响及氨基胍的保护效应[J].中国兽医杂志,2012,48(7):14-16.
[18] WATFORD M.Glutamine metabolism and function in relation to proline synthesis and the safety of glutamine and proline supplementation[J].The Journal of Nutrition,2008,138(10):2003S-2007S.
[19] BLACHIER F,GUIHOT-JOUBREL G,VAUGELADE P,et al.Portal hyperglutamatemia after dietary supplementation with monosodium glutamate in pigs[J].Digestion,1999,60(4):349-357.

[20] 史红超,苏铁柱.三羧酸循环及其影响因素对运动能力的影响[J].辽宁体育科技,2011,33(3):45-47,50.
[21] 李望,胡盛寿,魏英杰,等.心肌梗死对大鼠心肌能量代谢途径中关键酶的影响及意义[J].中国分子心脏病学杂志,2008,8(5):277-280.
[22] 朱国萍,黄恩启,赵旵军.NADP-异柠檬酸脱氢酶的结构与功能[J].安徽师范大学学报:自然科学版,2007,30(3):366-371.
[23] 陆兆丰.线粒体异柠檬酸脱氢酶和α-酮戊二酸脱氢酶系活性与创伤性脑损伤的实验研究[D].博士学位论文.上海:上海交通大学,2007.
[24] 石海峰.天冬氨酸对脂多糖刺激断奶仔猪肠道损伤的调控作用[D].硕士学位论文.武汉:武汉轻工大学,2013.
[25] PIERZYNOWSKI S G,SJODIN A.Perspectives of glutamine and its derivatives as feed additives for farm animals[J].Journal of Animal and Feed Sciences,1998,7(1):79-91.
[26] 刘坚,侯永清,丁斌鹰,等.α-酮戊二酸对脂多糖应激仔猪肠黏膜能量代谢的影响[J].动物营养学报,2009,21(6):892-896.
[27] HARDIE D G.Minireview:the AMP-activated protein kinase cascade:the key sensor of cellular energy status[J].Endocrinology,2003,144(12):5179-5183.

[28] EVANS A M,MUSTARD K J,WYATT C N,et al.Does AMP-activated protein kinase couple inhibition of mitochondrial oxidative phosphorylation by hypoxia to calcium signaling in O₂-sensing cells?[J].Journal of Biological Chemistry,2005,280(50):41504-41511.

[29] CANTÓ C,JIANG L Q,DESHMUKH A S,et al.Interdependence of AMPK and SIRT1 for metabolic adaptation to fasting and exercise in skeletal muscle[J].Cell Metabolism,2010,11(3):213-219.

[30] 张红胜,周玥.AMPK,SIRT1与能量代谢[J].国际病理科学与临床杂志,2009,29(3):202-206.
[31] JÄGER S,HANDSCHIN C,ST.-PIERRE J,et al.AMP-activated protein kinase (AMPK) action in skeletal muscle via direct phosphorylation of PGC-1alpha[J].Proceedings of the National Academy of Science of the United States of America,2007,104(29):12017-12022.

[32] LIANG H Y,WARD W F.PGC-1α:a key regulator of energy metabolism[J].Advances in Physiology Education,2006,30(4):145-151.

[33] 罗丽丽,刘振华,谢惠芳,等.SIRT1在脂多糖诱导的PC12细胞凋亡中的作用[J].中华神经医学杂志,2012,11(4):332-336.
[34] KANG P,LIU Y L,ZHU H L,et al.The effect of aspartate on the energy metabolism in the liver of weanling pigs challenged with lipopolysaccharide[J].European Journal of Nutrition,2015,54(4):581-588.

[35] WIJESEKARA N,TUNG A,THONG F,et al.Muscle cell depolarization induces a gain in surface GLUT4 via reduced endocytosis independently of AMPK[J].American Journal of Physiology,2006,290(6):E1276-E1286.

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