Effects of Lipoic Acid and Diquat on Growth Performance, Redox Status of Plasma and Jejunum and Cell Apoptosis of Jejunum of Finishing Pigs

BAO Weiguang; GU Xianhong; HAO Yue; CUI Yanjun; WANG Zhanbin

doi:10.3969/j.issn.1006-267x.2016.11.035

2016 , Vol. 28 >Issue 11: 3650 - 3659

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.11.035

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Effects of Lipoic Acid and Diquat on Growth Performance, Redox Status of Plasma and Jejunum and Cell Apoptosis of Jejunum of Finishing Pigs

BAO Weiguang ,
GU Xianhong ,
HAO Yue ,
CUI Yanjun ,
WANG Zhanbin

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1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China;
2. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2016-05-03

Online published: 2016-11-18

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Abstract

The aim of this study was to investigate the effects of lipoic acid (LA) and diquat on growth performance, redox status of plasma and jejunum and cell apoptosis of jejunum of finishing pigs. According to 2×2 double-factor test design, intraperitoneal injection of diquat (0 or 8 mg per kg body weight) and dietary LA (0 or 800 mg/kg) as the two main factors, and 4 groups were formed. Twenty-four healthy Large white castrates with the body weight of (70.64±3.61) kg were randomly allocated to 4 groups with 6 replicates per group and 1 pig per replicate. The trial period lasted for 29 days. LA were added in diets all in the trial. Intraperitoneal injection of diquat at day 15 and non-challenged pigs were injected intraperitoneally with the equal amount of physiological saline. Plasma and jejunum samples were collected at day 29 for determination of antioxidative indices using kits. Western blotting assays was conducted for determination of jejunum heat shock protein 70 (HSP70) and caspase-3 protein relative expression levels. The results showed as follows:1) there was a significant interaction of dietary LA and intraperitoneal injection of diquat on jejunum superoxide dismutase (SOD) activity of finishing pigs (P<0.01), and no significant interactions on growth performance, other antioxidant indices of plasma and jejunum and protein relative expression levels of HSP70 and caspase-3 of jejunum of finishing pigs were found (P>0.05). 2) Intraperitoneal injection of diquat significantly decreased average daily feed intake (ADFI) and average daily gain (ADG), the activities of SOD, glutathione peroxidase (GSH-Px) and catalase (CAT) of plasma, the activities of SOD and manganese superoxide dismutase (MnSOD) and total antioxidant capacity (T-AOC) of jejunum of finishing pigs (P<0.01); and significantly increased the ratio of feed to gain (F/G), the contents of malondialdehyde (MDA) of plasma and hydrogen peroxide (H₂O₂) of jejunum and protein relative expression levels of HSP70 and caspase-3 of jejunum of finishing pigs (P<0.01). Dietary LA significantly increased ADFI (P<0.05), ADG (P<0.01) and the activity of MnSOD of jejunum (P<0.01), and significantly decreased protein relative expression level of HSP70 of jejunum of finishing pigs (P<0.05), but had a tendency to decrease protein relative expression level of caspase-3 (P=0.052). 3) Dietary LA significantly increased ADFI and ADG (P<0.01), the activities of SOD of plasma (P<0.05) and SOD and MnSOD of jejunum (P<0.01) of finishing pigs challenged with diquat. In conclusion, intraperitoneal injection of diquat causes strong oxidative stress of finishing pigs, leads to the reduction of growth performance, imbalance of redox system of plasma and jejunum and cell apoptosis of jejunum. Dietary LA can suppress the oxidative stress caused by intraperitoneal injection of diquat, and may alleviate jejunum oxidation and apoptosis.

Key words： lipoic acid; diquat; finishing pigs; oxidative stress; jejunum; apoptosis

Cite this article

BAO Weiguang , GU Xianhong , HAO Yue , CUI Yanjun , WANG Zhanbin . Effects of Lipoic Acid and Diquat on Growth Performance, Redox Status of Plasma and Jejunum and Cell Apoptosis of Jejunum of Finishing Pigs[J]. Chinese Journal of Animal Nutrition, 2016 , 28(11) : 3650 -3659 . DOI: 10.3969/j.issn.1006-267x.2016.11.035

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