This study was conducted to evaluate the effects of dietary metabolizable energy (ME) and crude protein (CP) levels on laying performance, egg quality and plasma biochemical indexes of Shaoxing ducks, and to estimating the optimum levels of metabolizable energy and crude protein. Three dietary metabolizable energy levels (11.30, 10.88 and 10.46 MJ/kg) and three dietary crude protein levels (18.00%, 17.00% and 16.00%) were examined using a 3×3 factorial arrangement of treatments. A total of 972 healthy Shaoxing ducks were randomly allotted to 9 groups with 6 replicates per group and 18 birds per replicate. The experiment lasted for 12 weeks. The results showed as follows:1) dietary metabolizableenergy and crude protein levels did not affect the egg production, average egg weight average daily feed intake and deed to egg ratio (P>0.05). But dietary metabolizable energy level significantly affected daily egg mass (P<0.05). The best feed to egg ratio and daily egg mass can be obtained at dietary metabolizable energy level of 11.30 MJ/kg. The best egg production and daily egg mass can be obtained at dietary crude protein level of 18%. 2) The yolk color score was significantly increased with dietary metabolizable energy level increasing (P<0.05), but it was significantly decreased with dietary crude protein level increased (P<0.05). There was a interaction effect between dietary metabolizable energy and crude protein levels on the egg yolk color (P<0.05). 3) Dietary metabolizable energy and crude protein levels did not affect the plasma total protein and albumin content (P>0.05). The liver triglyceride content was significantly increased with dietary metabolizable energy level increasing (P<0.05). The liver lipid content trended to be decreased with dietary crude protein level increasing (P=0.08). The results indicate that the optimal dietary metabolizable energy and crude protein levels for performance of Shaoxing ducks was 11.30 MJ/kg and 18%, respectively.