Effects of Oral Administration of Ruminal Fluid Preparations on Immunoglobulin Contents in Intestinal Mucosa and Plasma of Lambs

WU Tingting; HAN Bing; NIE Biaobiao; DONG Weiwei; YANG Kailun

doi:10.3969/j.issn.1006-267x.2016.12.013

2016 , Vol. 28 >Issue 12: 3811 - 3818

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.12.013

Molecular Nutrition

Effects of Oral Administration of Ruminal Fluid Preparations on Immunoglobulin Contents in Intestinal Mucosa and Plasma of Lambs

WU Tingting ,
HAN Bing ,
NIE Biaobiao ,
DONG Weiwei ,
YANG Kailun

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1. Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, Xinjiang Agriculture University, Urumqi 830052, China;
2. Key Laboratory of Animal Biotechnology of Xinjiang, Key Laboratory of Genetics, Breeding and Reproduction of Grass-Feeding Animal, Ministry of Agriculture, Biotechnological Research Center, Xinjiang Academy of Animal Science, Urumqi 830000, China

Received date: 2016-06-28

Online published: 2016-12-17

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Abstract

This experiment aimed to study the effects of oral administration of ruminal fluid preparations on immunoglobulin contents in the intestinal mucosa and plasma of lambs, to explore the effects of ruminal fluid preparations on intestinal mucosal and humoral immunity of neonatal lambs. Fifty newborn lambs with similar birth weight as model animal were divided into 5 groups with 10 lambs per group. Lambs in trial groups were fed different preparations of ruminal fluid[ruminal fluid (Ⅰ), autoclaved ruminal fluid (Ⅱ), ultrasonic processing ruminal fluid (Ⅲ) and autoclaved ultrasonic processing ruminal fluid (Ⅳ)] from healthy adult sheep since 1 day of age once a day over a period of 5 days, and those in control group were given the same volume of sterile saline. Three lambs per group were slaughtered to obtain small intestinal tissues on 24 days of age; plasma was collected at 14 and 28 days of age, respectively. Immunoglobulin contents in intestinal mucosa and plasma were determined. The results showed as follows:1) in intestinal mucosal protein, the contents of immunoglobulin (IgA), secretory immunoglobin A (SIgA) and immunoglobulin G (IgG) in all trial groups were higher than those in control group. The total content of IgA in trial group Ⅲ was significantly higher than that in control group (P<0.01), as well as significantly higher than that in the other trial groups (P<0.05); there were no significant differences of the total contents of SIgA and IgG among groups (P>0.05). In generally, the contents of immunoglobulins showed ileum>duodenum>jejunum, and compared with control group, the total contents of immunoglobulins in trial group Ⅲ were greatly increased. 2) In plasma, immunoglobulin contents at 28 days of age were higher than those at 14 days of age. There was no significant difference of IgA content among groups at 14 days of age (P>0.05), however, at 28 days of age, the content of IgA in trial group Ⅰ was significantly increased compared with control group (P<0.05), and was significantly increased compared with the other trial groups (P<0.01); there was no significant difference of the content of IgG among groups at 14 and 28 days of age (P>0.05). In conclusion, orally administered different ruminal fluid preparations can improve intestinal mucosal immunity of newborn lambs, and ultrasonic processing ruminal fluid shows better effects than the other preparations.

Key words： ruminal fluid preparation; newborn lamb; intestinal mucosa; plasma; immunoglobulin

Cite this article

WU Tingting , HAN Bing , NIE Biaobiao , DONG Weiwei , YANG Kailun . Effects of Oral Administration of Ruminal Fluid Preparations on Immunoglobulin Contents in Intestinal Mucosa and Plasma of Lambs[J]. Chinese Journal of Animal Nutrition, 2016 , 28(12) : 3811 -3818 . DOI: 10.3969/j.issn.1006-267x.2016.12.013

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