A Study on Associative Effects of Three Cultivars of Stylosanthes with King Grass Using in Vitro Gas Production Technique

ZHANG Yage; LI Mao; ZHOU Hanlin; HU Lin; LI Wei; XU Tieshan

doi:10.3969/j.issn.1006-267x.2016.12.015

2016 , Vol. 28 >Issue 12: 3828 - 3835

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.12.015

Molecular Nutrition

A Study on Associative Effects of Three Cultivars of Stylosanthes with King Grass Using in Vitro Gas Production Technique

ZHANG Yage ,
LI Mao ,
ZHOU Hanlin ,
HU Lin ,
LI Wei ,
XU Tieshan

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1. College of Agriculture, Hainan University, Haikou 570100, China;
2. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China

Received date: 2016-05-27

Online published: 2016-12-17

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Abstract

This experiment was carried out to investigate the associative effects of three cultivars of stylosanthes with king grass using in vitro gas production technique. Three cultivars of stylosanthes (S. guianensis Sw. Reyan No. 2, S. guianensis Sw. Reyan No. 20 and S. guianensis Sw. Reyan No. 21) were combined with Pennisetum purpureum×P.americanum cv.Reyan No. 4 (king grass) in different proportions (0:100, 10:90, 20:80, 40:60, 50:50 and 100:0), respectively. The effects of combinations at different proportions on gas production, gas production parameters, in vitro dry matter digestibility (IVDMD), and ammonia nitrogen (NH₃-N) and microbial protein (MCP) contents were analyzed using in vitro gas production technique. The single-factors associative effects value and synthetically associative effects value of each combination were calculated to select the best proportions. The results showed as follows:1) significant differences were found in gas production of rapidly fermented fraction among different proportions of combinations of S. guianensis Sw. Reyan No. 2 and S. guianensis Sw. Reyan No. 20 with king grass (P<0.05), which reached the highest at proportions of 30:70 and 50:50, respectively. IVDMD reached the highest when S. guianensis Sw. Reyan No. 2:king grass and S. guianensis Sw. Reyan No. 20:king grass were 50:50 and 40:60, respectively, and were significantly different from that of king grass only (P<0.05). MCP content reached the highest when S. guianensis Sw. Reyan No. 2:king grass and S. guianensis Sw. Reyan No. 20:king grass were 30:70. NH₃-N content tended to be firstly increased and then decreased with the increase of stylosanthes proportion, and all of the combinations reached the highest at 30:70. 2) Synthetically associative effects value of three cultivars of stylosanthes with king grass all showed unimodal increase with the increase of stylosanthes proportion, and reached the highest when S. guianensis Sw. Reyan No. 2:king grass and S. guianensis Sw. Reyan No. 20:king grass were 30:70, and S. guianensis Sw. Reyan No. 21:king grass was 20:80. In conclusion, the supplementation of proper stylosanthes in king grass can improve gas production, IVDMD, MCP content and ruminal microbial activity, and acts positively associative effects; the effects are the best when S. guianensis Sw. Reyan No. 2:king grass and S. guianensis Sw. Reyan No. 20:king grass and S. guianensis Sw. Reyan No. 21:king grass are 30:70, 30:70 and 20:80, respectively.

Key words： stylosanthes; king grass; in vitro gas production; associative effects

Cite this article

ZHANG Yage , LI Mao , ZHOU Hanlin , HU Lin , LI Wei , XU Tieshan . A Study on Associative Effects of Three Cultivars of Stylosanthes with King Grass Using in Vitro Gas Production Technique[J]. Chinese Journal of Animal Nutrition, 2016 , 28(12) : 3828 -3835 . DOI: 10.3969/j.issn.1006-267x.2016.12.015

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