Appropriate Dietary Pantothenic Acid Level for Apis mellifera ligustica Worker Bee Larvae

LEI Chunhong; MA Lanting; WANG Hongfang; XU Baohua

doi:10.3969/j.issn.1006-267x.2016.12.017

Chinese Journal of Animal Nutrition >

2016 , Vol. 28 >Issue 12: 3846 - 3855

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.12.017

Molecular Nutrition

Appropriate Dietary Pantothenic Acid Level for Apis mellifera ligustica Worker Bee Larvae

LEI Chunhong ,
MA Lanting ,
WANG Hongfang ,
XU Baohua

Expand

College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China

Received date: 2016-06-13

Online published: 2016-12-17

Fold

Abstract

The objective of this experiment was to research the influences of dietary pantothenic acid level on development, body antioxidant function and the expressions of CoA synthesis related enzyme genes for Apis mellifera ligustica worker bee larvae, and to find the appropriate dietary pantothenic acid level in this stage. A total of 1 800 one-day-old Apis mellifera ligustica worker bee larvae were randomly assigned to 5 groups with 3 replicates per group and 120 larvae per replicate. Larvae in 5 groups were fed with experimental diets with 0.92, 1.22, 1.52, 1.82 and 2.12 mg/g (measured values) pantothenic acid until pupate, respectively. The body weight, body composition, haemolymph biochemical indices, antioxidant indices and the relative expression levels of CoA synthesis related enzyme genes of 5- and 7-day-old larvae were measured, and then the pupation and eclosion rates were calculated. The results showed as follows:1) pantothenic acid supplementation could significantly increase the fresh weight, dry weight and ether extract (EE) content of larvae (P<0.05), and eclosion rate in 2.12 mg/g group was significantly higher than that in other groups (P<0.05). 2) Dietary pantothenic acid level had significantly influences on total protein (TP), total cholesterol (TCHO), high density lipoprotein (HDL) and low density lipoprotein (LDL) contents in haemolymph of 5-day-old larvae (P<0.05), meanwhile, they reached the minimum in 1.82, 1.82, 1.22 and 1.52 mg/g groups, respectively. 3) The total anti-oxidant capacity (T-AOC) of 5-and 7-day-old larvae was significantly increased with the dietary pantothenic acid level increasing (P<0.05), as well as the superoxide dismutase (SOD) activity of 5-day-old larvae. 4) Dietary pantothenic acid level significantly influenced the relative expression levels of pantothenate kinase 4 (PANK4) and phosphopantothenoylcysteine decarboxylase (PPCDC) of 5-day-old larvae (P<0.05), and both of them reached the maximum in 1.52 mg/g group. Considering dry weight and eclosion rate of 5-day-old larvae to make fitting curves, the appropriate dietary pantothenic acid level for Apis mellifera ligustica worker bee larvae is 1.85 to 2.01 mg/g.

Key words： pantothenic acid; Apis mellifera ligustica; worker bee; larvae; appropriate level

Cite this article

LEI Chunhong , MA Lanting , WANG Hongfang , XU Baohua . Appropriate Dietary Pantothenic Acid Level for Apis mellifera ligustica Worker Bee Larvae[J]. Chinese Journal of Animal Nutrition, 2016 , 28(12) : 3846 -3855 . DOI: 10.3969/j.issn.1006-267x.2016.12.017

References

[1] TODD F E,BRETHERICK O.The composition of pollens[J].Journal of Economic Entomology,1942,35(3):312-317.

[2] LOPER G M,STANDIFER L N,THOMPSON M J,et al.Biochemistry and microbiology of bee-collected almond (Prunus dulcis) Pollen and bee bread.Ⅰ-fatty acids,sterols,vitamins and minerals[J].Apidologie,1980,11(1):63-73.

[3] 王俊芳,刘强,杨效民.泛酸在反刍动物生产中的应用[J].江西饲料,2008(3):16-17.

[4] 丁玉琴.泛酸钙对全饥饿鼠的保护作用及其机理研究[D].硕士学位论文.上海:第二军医大学,2001.

[5] LI L,FENG L,JIANG W D,et al.Dietary Pantothenic acid deficiency and excess depress the growth,intestinal mucosal immune and physical functions by regulating NF-κB,TOR,Nrf2 and MLCK signaling Pathways in grass carp (Ctenopharyngodon idella)[J].Fish & Shellfish Immunology,2015,45(2):399-413.

[6] 祁凤华,陈瑶,徐春生.泛酸对肉仔鸡营养物质代谢率影响的研究[J].黑龙江畜牧兽医:科技版,2009(15):36-37.

[7] 伊藤智夫,钟生泉,向仲怀.蚕的维生素营养[J].蚕学通讯,1985(2):12-19.

[8] 邱璐,郭俊生,赵法伋,等.泛酸钙、吡哆醛、叶酸及生物素对热暴露果蝇的保护作用[J].解放军预防医学杂志,2000,18(2):82-85.

[9] 罗雪雅.蜂王浆新鲜度指标筛选与缓解体力疲劳功能研究[D].硕士学位论文.重庆:西南大学,2008.

[10] KUO Y M,HAYFLICK S J,GITSCHIER J.Deprivation of pantothenic acid elicits a movement disorder and azoospermia in a mouse model of pantothenate kinase-associated neurodegeneration[J].Journal of Inherited Metabolic Disease,2007,30(3):310-317.

[11] 王荫长.昆虫生理学[M].北京:中国农业出版社,2004:65-65.

[12] Kanehisa L.Pentothenate and CoA bisynthesis[EB/OL].[2016-06-01].http:/www.kegg.jp/keggbin/showpathway?orgname=ame&mapno=00770&mapscale=&showdescription=hide,00770,9/21/16.

[13] VANDENBERG J D,SHIMANUKI H.Technique for rearing worker honeybees in the laboratory[J].Journal of Apicultural Research,1987,26(2):90-97.

[14] 张怡丁,张卫星,胥保华,等.人工饲养条件下意大利蜜蜂幼虫饲粮的适宜粗蛋白质水平[J].动物营养学报,2014,26(9):2722-2729.

[15] 马兰婷.代用花粉中α-亚麻酸水平对蜜蜂采食量、群势及脂质代谢的影响[D].硕士学位论文.泰安:山东农业大学,2013.

[16] 赵凤奎,胥保华,王红芳.意大利蜜蜂工蜂幼虫饲料中适宜色氨酸水平[J].中国农业科学,2015,48(7):1453-1462.

[17] LIN Y H,LIN H Y,SHIAU S Y.Estimation of dietary pantothenic acid requirement of grouper,Epinephelus malabaricus according to physiological and biochemical parameters[J].Aquaculture,2012,324-325:92-96.

[18] 刘安龙.草鱼幼鱼对饲料中核黄素、生物素和泛酸需要量的研究[D].硕士学位论文. 武汉:华中农业大学,2007.

[19] 黄凤,蒋明,文华,等.吉富罗非鱼对饲料中泛酸的需要量[J].水产学报,2014,38(9):1530-1537.

[20] ROEM A J,STICKNEY R R,KOHLER C C.Dietary pantothenic acid requirement of the blue tilapia[J].The Progressive Fish-Culturist,1991,53(4):216-219.

[21] SOLIMAN A K,WILSON R P.Water-soluble vitamin requirements of tilapia.1 pantothenic acid requirement of blue tilapia,Oreochromis aureus[J].Aquaculture,1992,104(1/2):121-126.

[22] BEER A E,SCOTT M L,NESHEIM M C.The effects of graded levels of pantothenic acid on the breeding performance of white leghorn pullets[J].British Poultry Science,1963,4(3):243-253.

[23] 张肖,王宝维,岳斌,等.泛酸对5-16周龄五龙鹅生长性能、屠宰性能、肌肉品质、营养物质利用率及血清生化指标的影响[J].动物营养学报,2015,27(11):3411-3419.

[24] WANG J P,YOO J S,KIM H J,et al.Nutrient digestibility,blood profiles and fecal microbiota are influenced by chitooligosaccharide supplementation of growing pigs[J].Livestock Science,2009,125(2/3):298-303.

[25] HAUNERLAND N H.Insect storage proteins:gene families and receptors[J].Insect Biochemistry and Molecular Biology,1996,26(8/9):755-765.

[26] 刘静,赵冬,秦兰萍,等.低密度脂蛋白胆固醇与心血管病发病关系的前瞻性研究[J].中华心血管病杂志,2001,29(9):561-565.

[27] 姜骅,唐成康.泛酸研究及其应用[J].四川食品与发酵,2004,40(1):11-13.

[28] SLYSHENKOV V S,RAKOWSKA M,MOISEENOK A G,et al.Pantothenic acid and its derivatives protect Ehrlich ascites tumor cells against lipid peroxidation[J].Free Radical Biology and Medicine,1995,19(6):767-772.

[29] BITZER-QUINTERO O K,DAVALOS-MARIN A J,ORTIZ G G,et al.Antioxidant activity of tryptophan in rats under experimental endotoxic shock[J].Biomedicine & Pharmacotherapy,2010,64(1):77-81.

[30] 杨延辉,赵建新,王浩,等.泛酸激酶及其抑制剂的研究进展[J].药学进展,2014,38(9):641-648.

[31] 张国滨,张晋,徐恒周,等.支链氨基酸氨基转移酶1在恶性胶质瘤中的表达及意义[J].中华神经外科杂志,2015,31(12).

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References