Porcine Glucagon-Like Peptide-2 Regulates Tight Junction Protein Expressions in Porcine Small Intestinal Epithelial Cell through Extracellular Regulated Kinase 1/2 Signaling Pathway

QI Keke; SUN Yuqing; WAN Jing; DENG Bo; MEN Xiaoming; WU Jie; XU Ziwei

doi:10.3969/j.issn.1006-267x.2016.12.024

2016 , Vol. 28 >Issue 12: 3912 - 3916

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.12.024

Molecular Nutrition

Porcine Glucagon-Like Peptide-2 Regulates Tight Junction Protein Expressions in Porcine Small Intestinal Epithelial Cell through Extracellular Regulated Kinase 1/2 Signaling Pathway

QI Keke ,
SUN Yuqing ,
WAN Jing ,
DENG Bo ,
MEN Xiaoming ,
WU Jie ,
XU Ziwei

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Institute of Animal Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received date: 2016-06-01

Online published: 2016-12-17

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Abstract

Porcine small intestinal epithelial cell from jejunum (IPEC-J2) was used to study the regulation of porcine glucagon-like peptide-2 (pGLP-2) on protein expressions of tight junction and its signaling pathway. Culture mediums were supplemented without (control group) or with 10^-9 mol/L pGLP-2 (pGLP-2 group), and 10^-9 mol/L pGLP-2 and 10 μmol/L U0126 (pGLP-2+U0126 group), respectively. Each group had 3 replicates with 1 culture pore per replicate. Protein expressions of zonula occludens-1 (ZO-1), occluding, claudin-1 and extracellular regulated kinase 1/2 (ERK1/2) in cytoplasm of IPEC-J2 were determined. The results showed as follows:compared with control group, the supplementation of pGLP-2 in culture medium of IPEC-J2 significantly increased protein expressions of ZO-1, claudin-1, occludin, p42-ERK1/2 and p44-ERK1/2 (P<0.05); compared with pGLP-2 group, the supplementation of U0126, an inhibitor of ERK1/2, in culture medium of IPEC-J2 significantly decreased protein expressions of the above proteins (P<0.05). The results suggest that ERK1/2 pathway is an important signaling pathway of pGLP-2 regulating tight junction protein expressions in intestinal epithelial cells.

Key words： porcine glucagon-like peptide-2; extracellular regulated kinase 1/2; tight junction protein

Cite this article

QI Keke , SUN Yuqing , WAN Jing , DENG Bo , MEN Xiaoming , WU Jie , XU Ziwei . Porcine Glucagon-Like Peptide-2 Regulates Tight Junction Protein Expressions in Porcine Small Intestinal Epithelial Cell through Extracellular Regulated Kinase 1/2 Signaling Pathway[J]. Chinese Journal of Animal Nutrition, 2016 , 28(12) : 3912 -3916 . DOI: 10.3969/j.issn.1006-267x.2016.12.024

References

[1] BURRIN D G,STOLL B,GUAN X.Glucagon-like peptide 2 function in domestic animals[J].Domestic Animal Endocrinology,2003,24(2):103-122.

[2] PEDERSEN N B,HJOLLUND K R,JOHNSEN A H,et al.Porcine glucagon-like peptide-2:structure,signaling,metabolism and effects[J].Regulatory Peptides,2008,146(1/2/3):310-320.

[3] DRUCKER D J,DEFOREST L,BRUBAKER P L.Intestinal response to growth factors administered alone or in combination with human[Gly²]glucagon-like peptide 2[J].American Journal of Physiology,1997,273(6):G1252-G1262.

[4] HSIEH J,LONGUET C,MAIDA A,et al.Glucagon-like peptide-2 increases intestinal lipid absorption and chylomicron production via CD36[J].Gastroenterology,2009,137(3):997-1005.

[5] 赵云.胰高血糖素样多肽-2的重组表达及其对肠道保护作用机制的实验研究[D].博士学位论文.重庆:第三军医大学,2006.

[6] YUSTA B,ESTALL J,DRUCKER D J.Glucagon-like peptide-2 receptor activation engages bad and glycogen synthase kinase-3 in a protein kinase A-dependent manner and prevents apoptosis following inhibition of phosphatidylinositol 3-kinase[J].Journal of Biological Chemistry,2002,277(28):24896-248906.

[7] KOEHLER J A,YUSTA B,DTUCKER D J.The Hela cell glucagon-like peptide-2 receptor is coupled to regulation of apoptosis and ERK1/2 activation through divergent signaling pathways[J].Molecular Endocrinology,2005,19(2):459-473.

[8] SHI X M,LI X J,WANG Y,et al.Glucagon-like peptide-2-stimulated protein synthesis through the PI 3-kinase-dependent Akt-mTOR signaling pathway[J].American Journal of Physiology,2011,300(3):E554-E563.

[9] DONG C X,ZHAO W,SOLOMON C,et al.The intestinal epithelial insulin-like growth factor-1 receptor links glucagon-like peptide-2 action to gut barrier function[J].Endocrinology,2014,155(2):370-379.

[10] MORAN G W,O'NEILL C,MCLAUGHLIN J T.GLP-2 enhances barrier formation and attenuates TNFα-induced changes in a Caco-2 cell model of the intestinal barrier[J].Regulatory Peptides,2012,178(1/2/3):95-101.

[11] WU J,QI K K,XU Z W,et al.Glucagon-like peptide-2-loaded microspheres as treatment for ulcerative colitis in the murine model[J].Journal of Microencapsulation,2015,32(6):598-607.

[12] 余长松,贾刚,邓秋红,等.胰高血糖素样肽-2对脂多糖应激的IPEC-J2细胞形态和紧密连接相关基因表达的影响[J].畜牧兽医学报,2015,46(4):592-599.

[13] GONZÁLEZ-MARISCAL L,TAPIA R,CHAMORRO D.Crosstalk of tight junction components with signaling pathways[J].Biochimica et Biophysica Acta,2008,1778(3):729-756.

[14] JASLEEN J,SHIMODA N,SHEN E R,et al.Signaling mechanisms of glucagon-like peptide 2-induced intestinal epithelial cell proliferation[J].Journal of Surgical Research,2000,90(1):13-18.

[15] JASLEEN J,ASHLEY S W,SHIMODA N,et al.Glucagon-like peptide 2 stimulates intestinal epithelial proliferation in vitro[J].Digestive Diseases and Sciences,2002,47(5):1135-1140.

[16] YUSTA B,SOMWAR R,WANG F,et al.Identification of glucagon-like peptide-2(GLP-2)-activated signaling pathways in baby hamster kidney fibroblasts expressing the rat GLP-2 receptor[J].The Journal of Biological Chemistry,1999,274(43):30459-30467.

[17] LI N,LIU B W,REN W Z,et al.GLP-2 attenuates LPS-induced inflammation in BV-2 cells by inhibiting ERK1/2,JNK1/2 and NF-κB signaling pathways[J].International Journal of Molecular Sciences,2016,17(2):190.

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