Effects of Dietary Selenium Content on Growth Performance, Antioxidant Capacity and Lipid Metabolism Gene Expression of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)

HU Junru; WANG Guoxia; SUN Yuping; CHEN Bing; CAO Junming; HUANG Yanhua; ZHUO Lixin

doi:10.3969/j.issn.1006-267x.2016.12.026

2016 , Vol. 28 >Issue 12: 3925 - 3934

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.12.026

Molecular Nutrition

Effects of Dietary Selenium Content on Growth Performance, Antioxidant Capacity and Lipid Metabolism Gene Expression of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)

HU Junru ,
WANG Guoxia ,
SUN Yuping ,
CHEN Bing ,
CAO Junming ,
HUANG Yanhua ,
ZHUO Lixin

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1. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China;
3. Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China

Received date: 2016-06-24

Online published: 2016-12-17

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Abstract

This experiment was conducted to investigate the effects of dietary selenium content on growth performance, antioxidant capacity and lipid metabolism gene expression of juvenile yellow catfish (Pelteobagrus fulvidraco). A total of 450 juvenile yellow catfish with the initial body weight of (2.12±0.01) g were randomly divided into 6 groups with 3 replicates per group and 20 fish per replicate. The fish in those groups were fed 6 isonitrogenous and isolipid experimental diets containing <0.05 (control group), 0.15, 0.23, 0.35, 0.47 and 0.96 mg/kg selenium for 8 weeks, respectively. The results showed as follows:the weight gain rate (WGR) increased firstly and decreased subsequently with dietary selenium content increasing, and the highest value appeared at the 0.23 mg/kg selenium group, which was significantly higher than that in control group (P<0.05). The feed conversion rate (FCR) exhibited an opposite trend to the WGR, and the 0.23 mg/kg selenium group had the lowest value, which was significantly lower than that in control group (P<0.05). The survival ratio in all groups was 100%. The highest whole body crude protein content was found in the 0.35 mg/kg selenium group, which was significantly higher than that in control group and 0.23 mg/kg selenium group (P<0.05). The whole body crude lipid content in control group was significantly lower than that in 0.15, 0.47 and 0.96 mg/kg selenium groups (P<0.05), but the whole body moisture content in control group was significantly higher than that in 0.15, 0.35, 0.47 and 0.96 mg/kg selenium groups (P<0.05). The plasma cholesterol (CHO) content in 0.23 mg/kg selenium group was the lowest, and was significantly lower than that in control group (P<0.05). The whole body selenium content and liver glutathione peroxidase (GPx) activity were enhanced with dietary selenium content increasing, and presented a dose-response relationship. The liver superoxide dismutase (SOD) activity was firstly increased and then decreased with dietary selenium content increased from <0.05 to 0.47 mg/kg, and then was increased again when dietary selenium content was 0.96 mg/kg. The relative expression levels of lipoprotein lipase (LPL) and lipoprotein lipase (FAS) mRNA in control group were significantly lower than those in 0.23 mg/kg selenium group (P<0.05). Using WGR as the evaluation index, the suitable dietary selenium content of juvenile yellow catfish is estimated to be 0.20 mg/kg by nonlinear regression analysis.

Key words： selenium; juvenile yellow catfish; growth performance; antioxidant capacity; lipid metabolism gene expression

Cite this article

HU Junru , WANG Guoxia , SUN Yuping , CHEN Bing , CAO Junming , HUANG Yanhua , ZHUO Lixin . Effects of Dietary Selenium Content on Growth Performance, Antioxidant Capacity and Lipid Metabolism Gene Expression of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)[J]. Chinese Journal of Animal Nutrition, 2016 , 28(12) : 3925 -3934 . DOI: 10.3969/j.issn.1006-267x.2016.12.026

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