Relationships between Contents of Nitrogenous Fractions of Cornell Net Carbohydrate and Protein System and Utilizable Amino Acids of Major Feeds for Dairy Cows

BAO Yu; HU Cong; ZHAO Guangyong

doi:10.3969/j.issn.1006-267x.2016.12.027

2016 , Vol. 28 >Issue 12: 3935 - 3942

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.12.027

Molecular Nutrition

Relationships between Contents of Nitrogenous Fractions of Cornell Net Carbohydrate and Protein System and Utilizable Amino Acids of Major Feeds for Dairy Cows

BAO Yu ,
HU Cong ,
ZHAO Guangyong

Expand

College of Animal Science and Technology, China Agricultural University, Beijing 100193, China

Received date: 2016-06-27

Online published: 2016-12-17

Fold

Abstract

To predict the utilizable amino acid (uAA) contents of major feeds for dairy cows, twenty-one different feed samples were collected and analyzed for the contents of crude protein (CP), ether extract (EE), neutral detergent fibre (NDF), acid detergent fibre (ADF), soluble crude protein (SCP), non-protein nitrogen (NPN), neutral detergent unsoluble crude protein (NDIP) and acid detergent unsoluble crude protein (ADIP). The contents of nitrogenous fractions including non-protein nitrogen (PA), rapidly degraded true protein (PB₁), intermediately degraded true protein (PB₂), slowly degraded true protein (PB₃) and unutilizable protein (PC) were calculated according to CNCPS. The contents of amino acids of feed samples were also analyzed. The contents of uAAs of feeds were calculated based on the relationships between feed amino acid and uAA. The results showed that the CNCPS nitrogenous fraction (PA, PB₁, PB₂, PB₃ and PC) contents (% DM) were highly correlated with uAA, utilizable methionine (uMet), utilizable lysine (uLys) and utilizable leucine (uLeu) contents (% DM), and the regression equations were as follows:uAA=0.171PA+0.134PB₁+0.364PB₂+0.370PB₃+0.148PC+7.785, R²=0.99, P<0.01; uMet=0.019PA-0.031PB₁+0.024PB₂+0.078PB₃-0.004PC-0.071, R²=0.93, P<0.01; uLys=-0.045PA-0.020PB₁+0.072PB₂+0.038PB₃-0.011PC+0.780, R²=0.92, P<0.01; uLeu=0.071PA+0.033PB₁+0.093PB₂+0.189PB₃+0.003PC+0.006, R²=0.94, P<0.01. It is concluded that uAA, uMet, uLys and uLeu of feeds for dairy cows can be predicted based on the CNCPS nitrogenous fraction contents and the equations established in the trial.

Key words： dairy cow; CNCPS; utilizable amino acid

Cite this article

BAO Yu , HU Cong , ZHAO Guangyong . Relationships between Contents of Nitrogenous Fractions of Cornell Net Carbohydrate and Protein System and Utilizable Amino Acids of Major Feeds for Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2016 , 28(12) : 3935 -3942 . DOI: 10.3969/j.issn.1006-267x.2016.12.027

References

[1] LAPIERRE H,PACHECO D,BERTHIAUME R,et al.What is the true supply of amino acids for a dairy cow?[J].Journal of Dairy Science,2006,89(S1):E1-E14.

[2] 赵广永,CHRISTENSEN D A,MCKINNON J J.用净碳水化合物——蛋白质体系评定反刍动物饲料营养价值[J].中国农业大学学报,1999,4(增刊):71-76.

[3] 曲永利,吴健豪,李铁.应用康奈尔净碳水化合物——蛋白质体系评定东北农区奶牛饲料营养价值[J].动物营养学报,2010,22(1):201-206.

[4] O'CONNOR J D,SNIFFEN C J,FOX D G,et al.A net carbohydrate and protein system for evaluating cattle diets:Ⅳ.Predicting amino acid adequacy[J].Journal of Animal Science,1993,71(5):1298-1311.

[5] PACHECO D,PATTON R A,PARYS C,et al.Ability of commercially available dairy ration programs to predict duodenal flows of protein and essential amino acids in dairy cows[J].Journal of Dairy Science,2012,95(2):937-963.

[6] ZHAO G Y,LEBZIEN P.The estimation of utilizable amino acids(uAA)of feeds for ruminants using an in vitro incubation technique[J].Journal of Animal Physiology and Animal Nutrition,2002,86(7/8):246-256.

[7] LICITRA G,HERNANDEZ T M,VAN SOEST P J.Standardization of procedures for nitrogen fractionation of ruminant feeds[J].Animal Feed Science and Technology,1996,57(4):347-358.

[8] AOAC.Official methods of analysis of AOCO international[M].16th ed.Arlington:AOCO International,1995.

[9] VAN SOEST P J,ROBERTSON J B,LEWIS B A.Methods for dietary fiber,neutral detergent fiber,and nonstarch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science,1991,74(10):3583-3597.

[10] 中华人民共和国国家质量监督检验检疫总局.GB/T 18246-2000饲料中氨基酸的测定[S].北京:中国标准出版社,2001.

[11] SNIFFEN C J,O'CONNOR J D,VAN SOEST P J,et al.A net carbohydrate and protein system for evaluating cattle diets:Ⅱ.Carbohydrate and protein availability[J].Journal of Animal Science,1992,70(11):3562-3577.

[12] LEBZIEN P,VOIGT J,GABEL M,et al.Zur schtzung der menge an nutzbarem rohprotein am duodenum von milchkühen[J].Journal of Animal Physiology and Animal Nutrition,1996,76(1/2/3/4/5):218-223.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References