Effects of Early Administration of Ruminal Fluid on Plasma Immunoglobins and Cytokines, and Mucosal Immunity-Associated Cells in Small Intestine in Lambs

WU Tingting; HAN Bing; LIU Zhen; HE Zhourui; YANG Kailun

doi:10.3969/j.issn.1006-267x.2016.12.028

2016 , Vol. 28 >Issue 12: 3943 - 3951

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.12.028

Molecular Nutrition

Effects of Early Administration of Ruminal Fluid on Plasma Immunoglobins and Cytokines, and Mucosal Immunity-Associated Cells in Small Intestine in Lambs

WU Tingting ,
HAN Bing ,
LIU Zhen ,
HE Zhourui ,
YANG Kailun

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1. Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, Xinjiang Agriculture University, Urumqi 830052, China;
2. Key Laboratory of Animal Biotechnology of Xinjiang, Key Laboratory of Genetics, Breeding and Reproduction of Grass-Feeding Animal, Ministry of Agriculture, Biotechnological Research Center, Xinjiang Academy of Animal Science, Urumqi 830000, China

Received date: 2016-06-02

Online published: 2016-12-17

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Abstract

This experiment aimed to study the effects of early administration of ruminal fluid on plasma immunoglobins and cytokines, and mucosal immunity-associated cells in small intestine in lambs. Twelve Texel newborn lambs were randomly allotted to two groups with 6 lambs per group. Ruminal fluid was orally administered once daily over a period of 14 days in trial group, and physiological saline was administered in control group. Blood was collected from jugular vein to separate plasma at 7, 14, 21 and 28 days of age, respectively; lambs were slaughtered to obtain small intestinal digesta at 28 days of age. Immunoglobins[immunoglobin A (IgA) and immunoglobin G (IgG)] and cytokines[interleukin-1β (IL-1β), interleukin-6(IL-6), tumor necrosis factor α (TNF-α) and interferon γ (IFN-γ)] contents in plasma, and IgG and immunoglobin Fc fragment (Fc) contents in intestinal digesta were determined. The results showed as follows:at 21 days of age, the contents of IgA and IgG in plasma in trial group was significantly lower than those in control group (P<0.05 or P<0.01), but there were no significant differences at the other days of ages (P>0.05); the contents of IL-1β and IL-6 in plasma in trial group were higher than those in control group, but the differences were not significant (P>0.05); plasma TNF-α content in trial group was significantly higher than that in control group at 14 days of age (P<0.05), but there were no significant differences at the other days of ages (P>0.05); plasma IFN-γ content in trial group was higher than that in control group, but the difference was not significant (P>0.05); there was no significant difference of IgG and Fc contents in intestinal digesta at 28 days of age (P>0.05); the administration of ruminal fluid increased intestinal intraepithelial lymphocyte count, especially in ileum, trial group was significantly different from control group (P<0.05); mast cell count at different intestinal segments was not significantly different between groups (P>0.05). It is concluded that early administration of ruminal fluid has no influence on humoral immune in lambs, while to some extent, can enhance cell-mediated immune; early administration of ruminal fluid has no influence on intestinal transport of maternal immunoglobins in lambs; early administration of ruminal fluid can stimulate intestinal mucosal immune system in lambs, and contribute to immunologic barrier establishment.

Key words： newborn lamb; ruminal fluid; plasma; immunoglobin; cytokine; mucosal immunity-associated cell

Cite this article

WU Tingting , HAN Bing , LIU Zhen , HE Zhourui , YANG Kailun . Effects of Early Administration of Ruminal Fluid on Plasma Immunoglobins and Cytokines, and Mucosal Immunity-Associated Cells in Small Intestine in Lambs[J]. Chinese Journal of Animal Nutrition, 2016 , 28(12) : 3943 -3951 . DOI: 10.3969/j.issn.1006-267x.2016.12.028

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