Molecular Nutrition

Regulatory Role of Glycine on Energy Metabolism and Its Related Genes' Expression in Liver of Piglets after Lipopolysaccharide Challenge

  • ZHANG Lin ,
  • WANG Xiuying ,
  • WU Huanting ,
  • WANG Haibo ,
  • CHEN Shaokui ,
  • ZHU Huiling ,
  • LIU Yulan
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  • Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China

Received date: 2016-06-02

  Online published: 2016-12-17

Abstract

This experiment was conducted to investigate the effects of glycine (Gly) on energy metabolism and the mRNA expression of the key enzymes and the regulatory factors involving in energy metabolism in liver of piglets after lipopolysaccharide (LPS) challenge. Twenty four Duroc×Landrace×Yorkshire piglets were randomly assigned into 4 groups with 6 replicates each and 1 pig in each replicate. The four groups were:1) control group (basal diet); 2) LPS group (LPS+basal diet); 3) 1.0% Gly group (LPS+basal diet+1.0% Gly); and 4) 2.0% Gly group (LPS+basal diet+2.0% Gly). On the 28th day, the piglets in experimental groups were injected intraperitoneally with 100 μg/kg BW LPS, and the piglets in the control group were injected with the same dose of physiological saline. All piglets were slaughtered at 4 h after LPS or saline injection, and the liver samples were collected for further analysis. The results showed as follows:1) compared with LPS group, supplementation of 1.0% Gly significantly increased liver ATP concentration and energy charge (EC) level of pigs (P<0.05), significantly decreased the ratio of AMP to ATP (P<0.05), and had a tendency to decrease AMP concentration (P<0.10); supplementation of 2.0% Gly had a tendency to decrease the ratio of AMP to ATP (P<0.10). 2) Compared with LPS group, supplementation of 1.0% Gly significantly decreased the mRNA expression of liver hexokinase 2 (Hexok2) and citroyl synthetase (CS) (P<0.05). In addition, supplementation of 2.0% Gly significantly decreased the mRNA expression of liver Hexok2 and pyruvate kinase (PK) (P<0.05), and had a tendency to decrease the mRNA expression of liver CS (P<0.10). 3) Compared with LPS group, supplementation of 1.0% Gly significantly increased the mRNA expression of hepatic AMP-activated protein kinase α1 (AMPKα1) (P<0.05). These results indicate that dietary supplementation of Gly can attenuate energy metabolism disorder caused by LPS stimulation in liver of weaning piglets, and regulates the gene expression of the key enzymes in glycolysis and tricarboxylic acid cycle.

Cite this article

ZHANG Lin , WANG Xiuying , WU Huanting , WANG Haibo , CHEN Shaokui , ZHU Huiling , LIU Yulan . Regulatory Role of Glycine on Energy Metabolism and Its Related Genes' Expression in Liver of Piglets after Lipopolysaccharide Challenge[J]. Chinese Journal of Animal Nutrition, 2016 , 28(12) : 4006 -4013 . DOI: 10.3969/j.issn.1006-267x.2016.12.035

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