Mitigative Effect of Dietary Selenium Content on Oxidative Stress of Ketotic Dairy Cows

ZHOU Yuanli; YE Gengping; LIU Guanglei

doi:10.3969/j.issn.1006-267x.2016.12.038

Chinese Journal of Animal Nutrition >

2016 , Vol. 28 >Issue 12: 4029 - 4035

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.12.038

Molecular Nutrition

Mitigative Effect of Dietary Selenium Content on Oxidative Stress of Ketotic Dairy Cows

ZHOU Yuanli ,
YE Gengping ,
LIU Guanglei

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1. Pet Science and Technology College, Jiangsu Agri-Animal Huabandry Vocational College, Taizhou 225300, China;
2. Shanghai Bright Holstan Co., Ltd., Shanghai 200436, China

Received date: 2016-07-26

Online published: 2016-12-17

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Abstract

The present study was aimed to evaluate the mitigative effect of dietary selenium (Se) content on oxidative stress of ketotic dairy cows, and to confirm the Se requirement of ketotic dairy cows. Forty Holstein dairy cows with (3.43±0.87)parities, (6.33±1.14) days in lactation, body weight of (644±36) kg, average milk yield of (38.42±4.82) kg/d and plasma β-hydroxybutyrate (BHBA) level of (1.50±0.26) mmol/L were assigned to 4 groups (n=10) according to a completely randomized design. The 1st group was control group, and the dairy cows in this group were fed a basal diet (Se content was 0.15 mg/kg DM); the 2nd, 3rd and 4th group were test groups, and the dairy cows in those groups were fed the basal diet added with sodium selenite (Na₂SeO₃) until the Se content was 0.30 (test group 1), 0.45 (test group 2) and 0.60 mg/kg DM (test group 3), respectively. The experiment period was divided into three periods and each period had 7 days. The blood samples were collected from all cows on the last day of each period, and the hepatic tissues were collected from five cows randomly selected from each group on the last day of the 2nd period. The results showed as follows:1) the Se content in whole blood and liver, the total antioxidant capacity (T-AOC), superoxide dismutase (SOD) activity, glutathione peroxidase (GPx) activity and the relative expression levels of GPx1 and GPx4 mRNA in liver of ketotic dairy cows in test groups were significantly higher than those in control group (P<0.05); meanwhile, those indexes in test groups 2 and 3 were significantly higher than those in test group 1 (P<0.05). 2) The malondialdehyde (MDA) and hydrogen peroxide (H₂O₂) contents in liver of ketotic dairy cows in test groups were significantly lower than those in control group (P<0.05); meanwhile, those indexes in test groups 2 and 3 were significantly lower than those in test group 1 (P<0.05). 3) No significant differences were found in plasma glucose and BHBA levels between test groups and control group (P>0.05), but the plasma not fat fatty acids (NEFA) level in test groups was significantly lower than that in control group (P<0.05). 4) There were no significant difference in the Se content in whole blood and liver, liver antioxidant indexes and plasma energy metabolism indicators of ketotic dairy cows when dietary Se contents were 0.45 and 0.60 mg/kg DM (P>0.05). In conclusion, diet containing 0.45 mg/kg DM Se can significantly improve the anti-oxidation ability of ketotic dairy cows.

Key words： ketotic dairy cows; Se; anti-oxidation; hepatic tissue; oxidative stress

Cite this article

ZHOU Yuanli , YE Gengping , LIU Guanglei . Mitigative Effect of Dietary Selenium Content on Oxidative Stress of Ketotic Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2016 , 28(12) : 4029 -4035 . DOI: 10.3969/j.issn.1006-267x.2016.12.038

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