Dipeptides Containing Methionine Affects Gene Expressions Involved in Milk Protein Synthesis in Bovine Mammary Epithelial Cells

CHEN Lu; CHANG Chencheng; SHI Binlin; GAO Min; ZHAO Yanli; YAN Sumei

doi:10.3969/j.issn.1006-267x.2016.12.039

2016 , Vol. 28 >Issue 12: 4036 - 4043

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.12.039

Molecular Nutrition

Dipeptides Containing Methionine Affects Gene Expressions Involved in Milk Protein Synthesis in Bovine Mammary Epithelial Cells

CHEN Lu ,
CHANG Chencheng ,
SHI Binlin ,
GAO Min ,
ZHAO Yanli ,
YAN Sumei

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1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China

Received date: 2016-06-07

Online published: 2016-12-17

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Abstract

The aim of this study was to investigate the effects of dipeptides containing methionine on gene expressions involved in milk protein synthesis in bovine mammary epithelial cells (BMECs). The study consisted of three parts and adopted completely random single-factor designs. Concentration of methionine and incubation time were 60 μg/mL (0.402 mmol/L) and 48 h, respectively. Part 1, eight kinds of dipeptides[methionine-methionine (P-Met-Met), methionine-lysine (P-Met-Lys), methionine-tryptophan (P-Met-Trp), methionine-phenylalanine (P-Met-Phe), methionine-threonine (P-Met-Thr), methionine-isoleucine (P-Met-Ile), methionine-leucine (P-Met-Leu) and methionine-valine (P-Met-Val)] were supplemented in culture medium, and culture medium without dipeptide supplementation was used as control; milk protein synthesis related geneα-casein (CSN1S1), β-casein (CSN2), κ-casein (CSN3), β-lactoglobulin (LGB), pep tide transporter 2 (PEPT2) and aminopeptidase nitrogen (APN) expressions in BMECs were determined. Part 2, eight kinds of free amino acids (F-Met-Met, F-Met-Lys, F-Met-Trp, F-Met-Phe, F-Met-Thr, F-Met-Ile, F-Met-Leu and F-Met-Val) corresponded with the above dipeptides, and culture medium without free amino acids supplementation was used as control; milk protein synthesis related gene expressions in BMECs were determined. Part 3, dipeptides containing methionine equimolarly substituted corresponding free amino acids; milk protein synthesis related gene expressions in BMECs and APA content inside and outside BMECs were determined. The results showed as follows:gene expressions of CSN1S1 and CSN2 were greater in P-Met-Met and P-Met-Lys groups than in other groups, and greater expressions were detected in P-Met-Met group than in P-Met-Lys group. Gene expressions of CSN1S1 was significantly increased in F-Met-Met and F-Met-Lys groups versus other groups (P<0.05). Except P-Met-Val and P-Met-Leu groups, dipeptides containing methionine substitution of corresponding free amino acids up-regulated the expressions of milk protein synthesis related genes and PEPT2 gene, and P-Met-Met had the best effect. In conclusion, dipeptides containing methionine equimolarly substitution of corresponding free amino acids can increase milk protein synthesis related gene expressions, especially P-Met-Met.

Key words： dairy cow; mammary epithelial cells; milk protein; methionine; dipeptide; gene expression

Cite this article

CHEN Lu , CHANG Chencheng , SHI Binlin , GAO Min , ZHAO Yanli , YAN Sumei . Dipeptides Containing Methionine Affects Gene Expressions Involved in Milk Protein Synthesis in Bovine Mammary Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2016 , 28(12) : 4036 -4043 . DOI: 10.3969/j.issn.1006-267x.2016.12.039

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