Molecular Nutrition

Effects of Yeast Culture on Growth Performance, Nonspecific Immunity and Disease Resistance of Litopenaeus vannamei

  • HE Yuanfa ,
  • YU Huanhuan ,
  • CHI Shuyan ,
  • YANG Qihui ,
  • LIU Hongyu ,
  • ZHANG Shuang ,
  • WANG Jia ,
  • TAN Beiping ,
  • DONG Xiaohui
Expand
  • 1. Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China;
    2. Beijing Enhalor Biotechnology Co., Ltd., Beijing 100081, China;
    3. South China Sea Bio-Resource Exploition and Utilization Collaborative Innovation Center, Guangzhou 510275, China

Received date: 2016-06-02

  Online published: 2016-12-17

Abstract

A 56 d ays feeding trial was carried out to investigate the effects of yeast culture on growth performance, nonspecific immunity and disease resistance of Litopenaeus vannamei. Four isonitrogenous and isolipid diets were prepared by adding 0, 0.30%, 0.50% and 1.00% yeast culture in a basal diet, and named as Y0, Y0.3, Y0.5 and Y1.0, respectively. A total of 800 Litopenaeus vannamei with an initial average weight of (1.20±0.01) g were randomly assigned into 4 groups with 5 replicates per group and 40 individuals per replicate. The results showed as follows:the weight gain rate (WGR) and specific growth rate (SGR) in Y0.3 group were significantly higher than those in Y0.5 group (P<0.05). The maximum protein efficiency rate (PER) and the minimum feed conversion rate (FCR) were occurred in Y0.3 group, and the differences were significant between Y0.3 group and other groups (P<0.05). Yeast culture had attractant effect at a certain extent, and the feeding rate (FR) in Y0.3 group showed significantly higher than that in Y0 group (P<0.05). Muscle crude protein content in Y0 group was significantly lower than that in Y0.3, Y0.5 and Y1.0 groups (P<0.05), and a highest value (91.69%) was found in Y0.3 group.The activities of serum lysozyme (LSZ), phenoloxidase (PO) and alkaline phosphatase (ALP) and liver LSZ, peroxidase (POD), superoxide dismutase (SOD) and ALP were significantly increased by dietary supplying 0.30%, 0.50% or 1.00% yeast culture (P<0.05), and serum malondialdehyde (MDA) content in Y0.5 group was significantly lower than that in other groups (P<0.05). Shrimps were challenged by Vibrio harveyi for the next 7 days, the cumulative mortality rate in Y0.3 and Y0.5 groups were significantly lower than that in Y1.0 group (P<0.05), but had no significant difference compared with Y0 group (P>0.05). It can be concluded that the supplementation of 0.30% yeast culture can significantly enhance the growth performance of Litopenaeus vannamei, and the supplementation of 0.30% to 0.50% yeast culture can significantly improve the nonspecific immunity of Litopenaeus vannamei.

Cite this article

HE Yuanfa , YU Huanhuan , CHI Shuyan , YANG Qihui , LIU Hongyu , ZHANG Shuang , WANG Jia , TAN Beiping , DONG Xiaohui . Effects of Yeast Culture on Growth Performance, Nonspecific Immunity and Disease Resistance of Litopenaeus vannamei[J]. Chinese Journal of Animal Nutrition, 2016 , 28(12) : 4063 -4072 . DOI: 10.3969/j.issn.1006-267x.2016.12.042

References

[1] MUROGA K.Viral and bacterial diseases of marine fish and shellfish in Japanese hatcheries[J].Aquaculture,2001,202(1/2):23-44.

[2] THITAMADEE S,PRACHUMWAT A,SRISALA J,et al.Review of current disease threats for cultivated penaeid shrimp in Asia[J].Aquaculture,2016,452:69-87.

[3] BURGENTS J E,BURNETT K G,BURNETT L E,et al.Disease resistance of Pacific white shrimp,Litopenaeus vannamei,following the dietary administration of a yeast culture food supplement[J].Aquaculture,2004,231(1/2/3/4):1-8.

[4] 刘立鹤,张恒,聂伟,等.草鱼配合饲料添加酵母培养物对草鱼生长性能、肝肠功能的影响[J].武汉轻工大学学报,2014,33(1):34-39.

[5] 张琴.刺参(Apostichopus japonicus Selenka)高效免疫增强剂的筛选与应用[D].博士学位论文.青岛:中国海洋大学,2010.

[6] YU H H,HAN F,XUE M,et al.Efficacy and tolerance of yeast cell wall as an immunostimulant in the diet of Japanese seabass (Lateolabrax japonicus)[J].Aquaculture,2014,432:217-224.

[7] JØRGENSEN J B,ROBERTSEN B.Yeast β-glucan stimulates respiratory burst activity of Atlantic salmon (Salmo salar L.) macrophages[J].Developmental & Comparative Immunology,1995,19(1):43-57.  

[8] MESHRAM S J,MURTHY H S,ALI H,et al.Effect of dietary β-glucan on immune response and disease resistance against Aeromonas hydrophila in giant freshwater prawn,Macrobrachium rosenbergii (de Man.1879)[J].Aquaculture International,2015,23(2):439-447.  

[9] NRC.Nutrition requirement of fish[S].Washington,D.C.:National Academy Press,2002:114.

[10] AOAC.Official methods of analysis[S].Arlington,VA:Association of Official Analytical Chemists,1995:684.

[11] ASHIDA M.Purification and characterization of pre-phenoloxidase from hemolymph of the silkworm Bombyx mori[J].Archives of Biochemistry and Biophysics,1971,144(2):749-762.  

[12] 郁欢欢,周文豪,曾虹,等.酵母培养物在水产动物中的应用及作用机理研究进展[J].饲料工业,2015,36(18):25-29.

[13] 赵贵萍.不同豆粕水平的饲料中添加一种酵母培养物(益康XP)对大菱鲆生长、组织学结构以及肠道菌群的影响[D].硕士学位论文.青岛:中国海洋大学,2008.

[14] 邱燕.三种微生态制剂对草鱼(Ctenopharyngodon idellus)生长性能、生理机能及肠道黏膜的影响[D].硕士学位论文.苏州:苏州大学,2010.

[15] 刘哲,魏时来.酵母培养物对建鲤生长性能影响的研究[J].饲料工业,2003,24(4):52-53.

[16] 温俊.复合益生菌与酵母培养物对牙鲆(Paralichthys olivaceus)生长、免疫及抗病力的影响[D].硕士学位论文.青岛:中国海洋大学,2007.

[17] 徐磊,刘波,谢骏,等.酵母培养物对异育银鲫生长、血液生化及免疫的影响[J].江苏农业科学,2010,(6):371-374.

[18] 李高锋.酵母培养物在团头鲂饲料中的应用研究[D].硕士学位论文.苏州:苏州大学,2009.

[19] 粟雄高,李小勤,冷向军,等.酵母培养物和芽孢杆菌对凡纳滨对虾生长、蛋白酶活性和免疫性能的影响[J].海洋渔业,2012,34(2):168-176.

[20] 粟雄高.柠檬酸和微生态制剂对凡纳滨对虾生长、消化酶活性和免疫性能的影响[D].硕士学位论文.上海:上海海洋大学,2012.

[21] 刘宗英,姚鹃,陈昌福,等.高活性干酵母对中华鳖非特异性免疫功能和抗病力的影响[J].华中农业大学学报,2005,24(2):192-196.

[22] YANG S P,WU Z H,JIAN J C,et al.Effect of marine red yeast Rhodosporidium paludigenum on growth and antioxidant competence of Litopenaeus vannamei[J].Aquaculture,2010,309(1/2/3/4):62-65.

[23] 邹玉蓉.降低中华鳖(Pelodiscus sinensis)配合饲料中鱼粉水平的研究[D].博士学位论文.青岛:中国海洋大学,2011.

[24] CAMPA-CÓRDOVA A I,HERNNDEZ-SAAVEDRA N Y,DE PHILIPPIS R,et al.Generation of superoxide anion and SOD activity in haemocytes and muscle of American white shrimp (Litopenaeus vannamei) as a response to β-glucan and sulphated polysaccharide[J].Fish & Shellfish Immunology,2002,12(4):353-366.  

[25] JENSEN G S,PATTERSON K M,YOON I.Yeast culture has anti-inflammatory effects and specifically activates NK cells[J].Comparative Immunology,Microbiology and Infectious Diseases,2008,31(6):487-500.  

[26] MOURÀO J L,PINHEIRO V,ALVES A,et al.Effect of mannan oligosaccharides on the performance,intestinal morphology and cecal fermentation of fattening rabbits[J].Animal Feed Science and Technology,2006,126(1/2):107-120.

[27] MUSSATTO S I,MANCILHA I M.Non-digestible oligosaccharides:a review[J].Carbohydrate Polymers,2007,68(3):587-597.  

[28] ELLIS A E.Immunity to bacteria in fish[J].Fish & Shellfish Immunology,1999,9(4):291-308.  

[29] 郑清梅,吴锐全,叶星.水生动物溶菌酶的研究进展[J].上海水产大学学报,2006,15(4):483-487.

[30] 杨留冰,潘鲁青.注射磷脂酰丝氨酸对凡纳滨对虾血蓝蛋白合成、酚氧化酶活性的影响[J].水产学报,2013,37(9):1378-1388.

[31] SUPHANTHARIKA M,KHUNRAE P,THANARDKIT P,et al.Preparation of spent brewer's yeast β-glucans with a potential application as an immunostimulant for black tiger shrimp,Penaeus monodon[J].Bioresource Technology,2003,88(1):55-60.  

[32] DUVIC B,SÖDERHÄLLÄK.β-1,3-glucan-binding proteins from plasma of the fresh-water crayfishes Astacus astacus and Procambarus clarkii[J].Journal of Crustacean Biology,1993,13(3):403-408.  

[33] WANG Y C.Expression of immune-related genes in the Pacific white shrimp,Litopenaeus vannamei and their odulation by beta-glucan via oral administration[D].Master Thesis.Kaohsiung:National Sun Yat-Sen University,2007.

[34] SARGIS R M,SUBBAIAH P V.Protection of membrane cholesterol by sphingomyelin against free radical-mediated oxidation[J].Free Radical Biology and Medicine,2006,40(12):2092-2102.  

[35] 张爱忠,卢德勋,姜宁,等.酵母培养物对绒山羊机体抗氧化能力的影响[J].动物营养学报,2010,22(3):781-786.

[36] 姚仕彬,叶元土,蔡春芳,等.酵母培养物水溶物对丙二醛损伤的离体草鱼肠道黏膜细胞的保护作用[J].动物营养学报,2014,26(9):2652-2663.

[37] SAFARI O,SHAHSAVANI D,PAOLUCCI M,et al.The effects of dietary nucleotide content on the growth performance,digestibility and immune responses of juvenile narrow clawed crayfish,Astacus leptodactylus Eschscholtz,1823[J].Aquaculture Research,2015,46(11):2685-2697.  

[38] HOSSAIN M S,KOSHIO S,ISHIKAWA M,et al.Dietary nucleotide administration influences growth,immune responses and oxidative stress resistance of juvenile red sea bream (Pagrus major)[J].Aquaculture,2016,455:41-49.

[39] XU L,RAN C,HE S X,et al.Effects of dietary yeast nucleotides on growth,non-specific immunity,intestine growth and intestinal microbiota of juvenile hybrid tilapia Oreochromis niloticus ♀×Oreochromis aureus♂[J].Animal Nutrition,2015,1(3):244-251.  

[40] 许丹丹,曹俊明,黄燕华,等.饲料中添加核苷酸对凡纳滨对虾幼虾生长、肠道形态及抗氧化酶活力的影响[J].中国水产科学,2011,18(5):1115-1124.
Outlines

/