Effects of Relative Humidity at Gradually Increasing Temperatures on Body Thermoregulation and Content of Heat Shock Protein 70 in Hypothalamus of Broiler Chickens

ZHOU Ying; ZHANG Minhong; FENG Jinghai; ZHANG Shaoshuai; PENG Qianqian; LI Meng; LI Xiang

doi:10.3969/j.issn.1006-267x.2017.01.008

2017 , Vol. 29 >Issue 1: 60 - 68

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.01.008

POULTRY NUTRITION

Effects of Relative Humidity at Gradually Increasing Temperatures on Body Thermoregulation and Content of Heat Shock Protein 70 in Hypothalamus of Broiler Chickens

ZHOU Ying ,
ZHANG Minhong ,
FENG Jinghai ,
ZHANG Shaoshuai ,
PENG Qianqian ,
LI Meng ,
LI Xiang

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1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Agriculture, Hebei University of Engineering, Handan 056021, China;
3. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

Received date: 2016-07-04

Online published: 2017-01-16

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Abstract

The present study aimed to investigate the effects of relative humidity (RH) at gradually increasing temperatures on body thermoregulation and the content of heat shock protein 70 (HSP70) in hypothalamus of broiler chickens. One hundred and eighty 21-day-old Arbor Acres (AA) broiler chickens were allotted into environment controlled chambers and randomly assigned to three groups with six replicates per group and ten broilers per replicate (males and females in half). The pre-test period lasted for 7 days and birds were kept at 20℃ and 60% RH. The test period lasted for 15 days, when broiler chickens were 28 days of age, RH of three groups was regulated to 35%, 60% and 85%, respectively, and the temperature was 20℃ yet. The experiment consisted of 5 consecutive trials with 3 days per consecutive trial, the temperature of three groups was gradually increased by 3℃ finishing within half an hour at 10:00 of the first day every consecutive trial and started from the second consecutive trial, and the temperatures of the 5 consecutive trials were 20, 23, 26, 29 and 32℃, respectively. The results showed as follows:1) the respiratory frequency of broiler chickens in the 85% RH group was significantly higher than that in the 60% RH and 35% RH groups (P<0.01), and the respiratory frequency in the 60% RH group was significantly higher than that in the 35% RH group (P<0.01). The core body temperature of broiler chickens in the 85% RH group was significantly higher than that in the 60% RH group (P<0.05). The skin temperatures of leg and flipper of broiler chickens in the 35% RH group were significantly higher than those in the 60% RH and 85% RH groups (P<0.01), and the skin temperatures of comb, earlobe and eyelid in the 35% RH and 60% RH groups were significantly higher than those in the 85% RH group (P<0.01). 2) The activity of alkaline phosphatase (AKP) in serum of broiler chickens in the 85% RH group was significantly higher than that in the 60% RH and 35% RH groups (P<0.05), and the activity of creatine kinase (CK) in serum of broiler chickens in the 85% RH and 35% RH groups was significantly higher than that in the 60% RH group (P<0.05). There were no significant effects of RH at gradually increasing temperatures on the activities aspartate transaminase (AST) and lactate dehydrogenase (LDH) in serum of broiler chickens (P>0.05). 3) The content of 3,5,3'-triiodothyronine (T₃) in serum of broiler chickens in the 85% RH group was significantly higher than that in the 35% RH group (P<0.05), the content of 3,5,3',5'-tetraiodothyronine (T₄) in serum in the 35% RH and 85% RH groups was significantly higher than that in the 60% RH group (P<0.05), and the content of cortisol (CORT) in serum in the 60% RH and 85% RH groups was significantly higher than that in the 35% RH group (P<0.01). 4) The content of HSP70 in hypothalamus of broiler chickens in the 35% RH group was significantly lower than that in the 60% RH and 85% RH groups (P<0.05). In conclusion, at the 15-day 20 to 32℃ gradually increasing temperatures, high humidity (85%) and low humidity (35%) regulate the body heat balance of broiler chickens at 28 to 42 days of age in different ways and extent.

Key words： gradually increasing temperatures; broiler chickens; relative humidity; body thermoregulation; heat shock protein 70

Cite this article

ZHOU Ying , ZHANG Minhong , FENG Jinghai , ZHANG Shaoshuai , PENG Qianqian , LI Meng , LI Xiang . Effects of Relative Humidity at Gradually Increasing Temperatures on Body Thermoregulation and Content of Heat Shock Protein 70 in Hypothalamus of Broiler Chickens[J]. Chinese Journal of Animal Nutrition, 2017 , 29(1) : 60 -68 . DOI: 10.3969/j.issn.1006-267x.2017.01.008

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