Exploration of Requirement Models and Limiting Sequence of Four Kinds of Essential Amino Acids of Weaned Lambs

LI Xueling; CHAI Jianmin; ZHANG Naifeng; ZHANG Rong; WANG Jie; WANG Shiqin; TAO Dayong; DIAO Qiyu

doi:10.3969/j.issn.1006-267x.2017.01.013

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 1: 106 - 117

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.01.013

RUMINANT AND HERBIVORE NUTRITION

Exploration of Requirement Models and Limiting Sequence of Four Kinds of Essential Amino Acids of Weaned Lambs

LI Xueling ,
CHAI Jianmin ,
ZHANG Naifeng ,
ZHANG Rong ,
WANG Jie ,
WANG Shiqin ,
TAO Dayong ,
DIAO Qiyu

Expand

1. College of Animal Science, Tarim University, Alar 843300, China;
2. Feed Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Beijing 100081, China

Received date: 2016-07-12

Online published: 2017-01-16

Fold

Abstract

This study was conducted to investigate the requirement models and sequence of 4 kinds of essential amino acids lysine (Lys), methionine (Met), threonine (Thr) and tryptophan (Trp) in weaned lambs by partial deduction method. One hundred male Hu lambs weaned at 50 days of age were randomly divided into five groups, and each group had four replicates with five lambs per replicate. Control group was fed a starter with balanced amino acid (PC group), while 4 experimental groups were fed the starter deducting Lys (PD-Lys group), Met (PD-Met group), Thr (PD-Thr group) and Trp (PD-Trp group) on the basis of PC group, respectively. The pretrial lasted for 10 days, and the formal trial lasted for 60 days. All the lambs were weighed at 60, 90 and 120 days of age, and six lambs selected from each group were slaughtered at 120 days of age. The results showed as follows:1) at 60 to 120 days of age and 90 to 120 days of age, average daily gain (ADG) in PD-Met group was significantly lower than that in the other groups (P<0.05), and feed to gain ratio (F/G) in PD-Met group was significantly higher than that in the other groups (P<0.05); at 120 days of age, PD-Met group had significantly lower live weight before slaughter, empty body weight, carcass weight, ribeye area, head weight, feet weight, and skin and wool weight compared with PC, PD-Thr and PD-Trp groups (P<0.05), however, no significant differences of dressing percentage (DP), GR value and blood weight among five groups at 120 days of age were found (P>0.05). 2) Using optimal ADG and F/G as the evaluation indexes, limited amino acid sequence was Met, Lys, Thr and Trp of lambs at 60 to 90 days of age and 90 to 120 days of age; using optimal DP as the evaluation index, limited amino acid sequence was Trp, Thr, Lys and Met of lambs at 120 days of age. 3) Using optimal ADG as the evaluation index, the proper proportion of Lys, Met, Thr and Trp was 100:44:44:8 at 60 to 90 days of age and 100:42:38:12 at 90 to 120 days of age; using optimal F/G as the evaluation index, the proper proportion of Lys, Met, Thr and Trp was 100:54:45:7 at 60 to 90 days of age and 100:47:39:12 at 90 to 120 days of age; using optimal DP as the evaluation index, the proper proportion of Lys, Met, Thr and Trp was 100:34:38:8 at 60 to 120 days of age. In conclusion, dietary lack of essential amino acids, such as Lys, Met, Thr and Trp, can decrease growth performance, slaughter performance and organ indexes of lambs, the lack of Met is most negative and the next is Lys, however the effects of Thr and Trp were weak; the limited sequences and proper proportions of Lys, Met, Thr and Trp are different when evaluated using different indexes (ADG, F/G and DP) at different growth stages.

Key words： Hu sheep; weaned lamb; starter; amino acid model; lysine; methionine; tryptophan

Cite this article

LI Xueling , CHAI Jianmin , ZHANG Naifeng , ZHANG Rong , WANG Jie , WANG Shiqin , TAO Dayong , DIAO Qiyu . Exploration of Requirement Models and Limiting Sequence of Four Kinds of Essential Amino Acids of Weaned Lambs[J]. Chinese Journal of Animal Nutrition, 2017 , 29(1) : 106 -117 . DOI: 10.3969/j.issn.1006-267x.2017.01.013

References

[1] 刁其玉.动物氨基酸营养与饲料[M].北京:化学工业出版社,2007.
[2] 印遇龙.氨基酸新功能及其对脂质代谢和能量平衡的调控[J].饲料与畜牧规模养猪,2016(6):17-21.
[3] 李华伟,祝倩,吴灵英,等.色氨酸的生理功能及其在畜禽饲粮中的应用[J].动物营养学报,2016,28(3):659-664.
[4] 张乃锋,李辉,屠焰,等.氨基酸对早期断奶犊牛生长性能及氮代谢的影响[C]//中国畜牧兽医学会动物营养学分会第十一次全国动物营养学术研讨会论文集.北京:中国畜牧兽医学会动物营养学分会,2012.
[5] 王建红,刁其玉,许先查,等.日粮Lys、Met和Thr添加模式对0-2月龄犊牛生长性能、消化代谢与血清学生化指标的影响[J].中国农业科学,2011,44(9):1898-1907.
[6] KERR B J,YEN J T,NIENABER J A,et al.Influences of dietary protein level,amino acid supplementation and environmental temperature on performance,body composition,organ weights and total heat production of growing pigs[J].Journal of Animal Science,2003,81(8):1998-2007.

[7] ZHANG G J,XIE C Y,THACKER P A,et al.Estimation of the ideal ratio of standardized ileal digestible threonine to lysine for growing pigs (22-50 kg) fed low crude protein diets supplemented with crystalline amino acids[J].Animal Feed Science and Technology,2013,180(1/2/3/4):83-91.
[8] 王建红.0-2月龄犊牛代乳品中赖氨酸、蛋氨酸和苏氨酸适宜模式的研究[D].硕士学位论文.北京:中国农业科学院,2011.
[9] TIEMANN T T,LASCANO C E,WETTSTEIN H R,et al.Effect of the tropical tannin-rich shrub legumes Calliandra calothyrsus and Flemingia macrophylla on methane emission and nitrogen and energy balance in growing lambs[J].Animal,2008,2(5):790-799.
[10] TEDESCHI L O,FOX D G,FONSECA M A,et al.Models of protein and amino acid requirements for cattle[J].Revista Brasileira de Zootecnia,2015,44(3):109-132.

[11] WANG T C,FULLER M F.The effect of the plane of nutrition on the optimum dietary amino acid pattern for growing pigs[J].Animal Production,1990,50(1):155-164.

[12] MABJEESH S J,GUY D,SKLAN D.Na⁺/glucose co-transporter abundance and activity in the small intestine of lambs:enhancement by abomasal infusion of casein[J].British Journal of Nutrition,2003,89(5):573-580.

[13] OBEIDAT B S,ABDULLAH A Y,AWAWDEH M S,et al.Effect of methionine supplementation on performance and carcass characteristics of Awassi ram lambs fed finishing diets[J].Asian-Australasian Journal of Animal Sciences,2008,21(6):831-837.

[14] STORM E,ØRSKOV E R.The nutritive value of rumen micro-organisms in ruminants.4.The limiting amino acids of microbial protein in growing sheep determined by a new approach[J].British Journal of Nutrition,1984,52(3):613-620.

[15] 张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007.
[16] MCMILLAN M L.The effects of bermudagrass-clipping pellets as an alternative forage source in the diets of sheep[D].Ph.D.Thesis.Texas:Texas Tech University,2004.
[17] UNNIKRISHNAN U,PAULRAJ R.Dietary protein requirement of giant mud crab Scylla serrata juveniles fed iso-energetic formulated diets having graded protein levels[J].Aquaculture Research,2010,41(2):278-294.

[18] 祁敏丽,柴建民,王波,等.饲粮营养限制对早期断奶湖羊羔羊生长性能以及内脏器官发育的影响[J].动物营养学报,2016,28(2):444-454.
[19] 王建红,刁其玉,许先查,等.赖氨酸、蛋氨酸和苏氨酸对犊牛生长性能和血清生化指标的影响[J].动物营养学报,2011,23(2):226-233.
[20] EL-TAHAWY A S,ISMAEIL A M,AHMED H A.Effects of dietary methionine-supplementation on the general performance and economic value of rahmani lambs[J].Journal of Animal Science Advances,2015,5(10):1457-1466.

[21] NOLTE J V E,LÖEST C A,FERREIRA A V,et al.Methionine,and at least one branchedchain amino acid,are limiting in lambs[J].Journal of Animalence,2004,82(1):130-131.
[22] NOLTE J V E,LÖEST C A,FERREIRA A V,et al.Limiting amino acids for growing lambs fed a diet low in ruminally undegradable protein[J].Journal of Animal Science,2008,86(10):2627-2641.

[23] FERREIRA A V,VAN DER MERWE H J,FAIR M D.Nitrogen retention response to the abomasal infusion of amino acids in South African Mutton Merino lambs[J].South African Journal of Animal Science,2002,32(2):106-112.
[24] WANG J H,HUNG C H,YOUNG T H.Proliferation and differentiation of neural stem cells on lysine-alanine sequential polymer substrates[J].Biomaterials,2006,27(18):3441-3450.

[25] BOUYEH M.Effects of excess dietary lysine and methionine on performance and economical efficiency of broiler chicks[J].Annals of Biological Research,2013,4(5):241-246.
[26] 张乃锋,李辉,屠焰,等.氨基酸对早期断奶犊牛生长性能及氮代谢的影响[C]//中国畜牧兽医学会动物营养学分会第十一次全国动物营养学术研讨会论文集.长沙:中国畜牧兽医学会动物营养学分会,2012.
[27] 冯杰,许梓荣.苏氨酸与赖氨酸不同比例对猪生长性能和胴体组成的影响[J].浙江大学学报:农业与生命科学版,2003,29(1):14-17.
[28] LV K,HOU S Z,WANG Z Y,et al.Dietary Protein level and Lys/Met affect growth performance and gastrointestinal morphology of early-weaner Tibetan lambs[J].Chinese Journal of Animal Nutrition,2013,25(4):743-751.
[29] 李辉.蛋白水平与来源对早期断奶犊牛消化代谢及胃肠道结构的影响[D].博士学位论文.北京:中国农业科学院,2008.
[30] GOODBAND B,TOKACH M,DRITZ S,et al.Practical starter pig amino acid requirements in relation to immunity,gut health and growth performance[J].Journal of Animal Science and Biotechnology,2014,5(1):12.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References