Requirements of Vitamin B1 and Vitamin B2 of 12-Month-Old Yili Horses

CHEN Junhong; HE Xueman; DENG Haifeng; LI Xiaobin; LIU Zhen; MA Jun; GAN Jianrong; TANG Xuemei; YANG Kailun

doi:10.3969/j.issn.1006-267x.2017.01.014

Chinese Journal of Animal Nutrition >

2017 , Vol. 29 >Issue 1: 118 - 126

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.01.014

RUMINANT AND HERBIVORE NUTRITION

Requirements of Vitamin B₁ and Vitamin B₂ of 12-Month-Old Yili Horses

CHEN Junhong ,
HE Xueman ,
DENG Haifeng ,
LI Xiaobin ,
LIU Zhen ,
MA Jun ,
GAN Jianrong ,
TANG Xuemei ,
YANG Kailun

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Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, Xinjiang Agricultural University, Urumqi 830052, China

Received date: 2016-07-12

Online published: 2017-01-16

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Abstract

In order to get the vitamin B₁ and vitamin B₂ requirements of Yili horses, the effects of different intakes of vitamin B₁ and vitamin B₂ on blood and urinary related indices of 12-month-old Yili horses were studied. Thirty-five healthy male Yili horses with the age of 12 months±5 days and the average body weight of (245.28±18.36) kg were randomly divided into 5 groups (trial groups Ⅰ, Ⅱ, Ⅲ, Ⅳ and Ⅴ), and each group had 7 horses. The horses in trail groups Ⅰ, Ⅱ, Ⅲ, Ⅳ and Ⅴ were fed the same basal diet added 0, 16, 32, 48 and 64 mg/(horse·d) of vitamin B₁ and 0, 10, 20, 30 and 40 mg/(horse·d) of vitamin B₂ for 20 days, respectively. The practical intakes of vitamin B₁ and vitamin B₂ in trail groups Ⅰ, Ⅱ, Ⅲ, Ⅳ and Ⅴ were 19.00, 33.49, 48.27, 62.96, 77.53 mg/(horse·d) and 21.95, 31.68, 41.77, 51.53, 61.26 mg/(horse·d), respectively. The results showed as follows:with the practical intake of vitamin B₁ increasing, plasma vitamin B₁ content, erythrocyte transketolase activity (E-TKA) and urinary vitamin B₁ output increased gradually, while the thiamin pyrophosphate effect (TPP-effect) decreased gradually. The plasma vitamin B₁ content in trail group Ⅰ was significantly lower than that in trail group Ⅲ (P<0.05), and extremely significantly lower than that in trail groups Ⅳ and Ⅴ (P<0.01). The E-TKA in trail group Ⅰ was extremely significantly lower than that in trail groups Ⅲ, Ⅳ and Ⅴ (P<0.01). The TPP-effect in trail group Ⅰ was significantly higher than that in trail group Ⅲ (P<0.05), and extremely significantly higher than that in trail groups Ⅳ and Ⅴ (P<0.01). The urinary vitamin B₁ output in trail group Ⅰ was extremely significantly lower than that in trail groups Ⅲ, Ⅳ and Ⅴ (P<0.01). With the practical intake of vitamin B₂ increasing, the plasma vitamin B₂ content showed a fluctuating change, the erythrocyte glutathione reductase activity coefficient (E-GRAC) decreased gradually, and the urinary vitamin B₁ output increased gradually. The plasma vitamin B₂ content in trail group Ⅰ was significantly lower than that in trail groups Ⅲ and Ⅳ (P<0.05), and extremely significantly lower than that in trail group Ⅴ (P<0.01). The E-GRAC in trail group Ⅰ was extremely significantly higher than that in trail groups Ⅱ, Ⅲ,Ⅳ and Ⅴ (P<0.01). The urinary vitamin B₂ output in trail group Ⅰ was extremely significantly lower than that in trail groups Ⅲ, Ⅳ and Ⅴ (P<0.01). Base on the results of this study, the optimal requirements of vitamin B₁ and vitamin B₂ are 48.27 and 31.68 mg/(horse·d) for 12-month-old Yili horses, respectively.

Key words： Yili horses; vitamin B₁; vitamin B₂; requirement

Cite this article

CHEN Junhong , HE Xueman , DENG Haifeng , LI Xiaobin , LIU Zhen , MA Jun , GAN Jianrong , TANG Xuemei , YANG Kailun . Requirements of Vitamin B₁ and Vitamin B₂ of 12-Month-Old Yili Horses[J]. Chinese Journal of Animal Nutrition, 2017 , 29(1) : 118 -126 . DOI: 10.3969/j.issn.1006-267x.2017.01.014

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