Effects of Dietary Linolenic Acid Content on Growth, Antioxidant Capacity, Non-Specific Immunity and Anti-Ammonia-Nitrite Stress Ability of Oriental River Prawn (Macrobrachium nipponense)

LUO Na; DING Zhili; ZHANG Yixiang; KONG Youqin; WU Chenglong; JIANG Zhiqiang; YE Jinyun

doi:10.3969/j.issn.1006-267x.2017.01.016

2017 , Vol. 29 >Issue 1: 134 - 146

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.01.016

AQUACULTURE NUTRITION

Effects of Dietary Linolenic Acid Content on Growth, Antioxidant Capacity, Non-Specific Immunity and Anti-Ammonia-Nitrite Stress Ability of Oriental River Prawn (Macrobrachium nipponense)

LUO Na ,
DING Zhili ,
ZHANG Yixiang ,
KONG Youqin ,
WU Chenglong ,
JIANG Zhiqiang ,
YE Jinyun

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1. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116000, China;
2. Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition of Chinese Academy of Fishery Sciences, College of Life Science, Huzhou University, Huzhou 313000, China

Received date: 2016-07-18

Online published: 2017-01-16

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Abstract

Linolenic acid (C18:3n-3, LNA) is one of the important polyunsaturated fatty acids (PUFA) for the regulation of growth, immunity and environmental stress resistance of crustacean. This experiment was investigated to study the effects of dietary LNA content on growth, antioxidant capacity, non-specific immunity and anti-ammonia-nitrite stress ability of oriental river prawn (Macrobrachium nipponense), and to discuss the suitable dietary LNA content. LNA was added to the diets to formulate six isonitrogenous and isolipidic semipurified diets containing 0 (L0, control), 0.5% (L0.5), 1.0% (L1.0), 1.5% (L1.5), 2.0% (L2.0) and 2.5% (L2.5) LNA, respectively. Each diets fed 5 tanks (replicates) with 50 prawns per tank. After 8 weeks of feeding, ten prawns from each tank were exposed to ammonia nitrogen (total ammonia nitrogen concentration in water was 36.6 mg/L) for 24 h. The results showed as follows:with the dietary LNA content increasing, the specific growth rate, weight gain rate and survival rate of prawns were increased firstly and then decreased, but the differences were not significant among all groups (P>0.05). The contents of LNA in hepatopancreas and muscle were all increased with the dietary LNA content increasing. The superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activities and total antioxidant capacity (T-AOC) in hepatopancreas basically showed a trend of first increasing and then decreasing with the dietary LNA content increasing, and all of them reached the highest values in L1.0 group. The hepatopancreas malondialdehyde (MDA) content in L0.5, L1.0, L1.5, L2.0 and L2.5 groups was significantly lower than that in L0 group (P<0.05). The prawn fed the diet with 1.0% LNA showed the highest hepatopancreas acid phosphatase (ACP), however, there was no significant difference between L1.0 and L1.5 groups (P>0.05). Hepatopancreas lysozyme (LYZ) activity increased at first and then decreased with the dietary LNA content increasing, and it reached the highest activity in L1.5 group which was significantly higher than that in other groups (P<0.05). After 24 h ammonia-nitrite stress, hepatopancreas MDA content in L0.5, L1.5, L2.0 and L2.5 groups was significantly lower than that in L0 group (P<0.05), and the L1.5 group had the lowest MDA content which was significantly lower than that in other groups (P<0.05); hepatopancreas SOD activity and T-AOC showed a trend of first increasing and then decreasing with the dietary LNA content increasing, and them reached the highest values in L1.5 and L1.0 groups, respectively; hepatopancreas GSH-Px activity reached the highest value in L0 group, however, there was no significant difference between L0 and L1.0 groups (P>0.05). Taking hepatopancreas SOD activity as the indicator, the requirement of LNA for oriental river prawn was 1.19% according to quadratic regression analysis. As a consequence of the above, the suitable dietary LNA content (1.0% to 1.5%) can improve the growth, antioxidant capacity, non-specific immunity, and can relax the negative effect caused by anti-ammonia-nitrite stress of oriental river prawn.

Key words： oriental river prawn (Macrobrachium nipponense); linolenic acid; growth; antioxidant; non-specific immune; anti-ammonia-nitrite stress

Cite this article

LUO Na , DING Zhili , ZHANG Yixiang , KONG Youqin , WU Chenglong , JIANG Zhiqiang , YE Jinyun . Effects of Dietary Linolenic Acid Content on Growth, Antioxidant Capacity, Non-Specific Immunity and Anti-Ammonia-Nitrite Stress Ability of Oriental River Prawn (Macrobrachium nipponense)[J]. Chinese Journal of Animal Nutrition, 2017 , 29(1) : 134 -146 . DOI: 10.3969/j.issn.1006-267x.2017.01.016

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