MOLECULAR NUTRITION

Effects of Deoxynivalenol on Intestinal Microbiota of Mice Analyzed by Illumina-MiSeq High-Throughput Sequencing Technology

  • YANG Junhua ,
  • ZHAO Zhihui ,
  • GUO Wenbo ,
  • GUO Jinli
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  • Institute of Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China

Received date: 2016-07-21

  Online published: 2017-01-16

Abstract

This study was conducted to investigate the effects of deoxynivalenol (DON) on bacterial diversity and species abundance of intestinal microbiota. A total of 40 BALB/c mice weighted about 20 g were randomly allotted to control group (intragastric gavage sterilized saline) and DON group (intragastric gavage 1.8 mg/kg BW DON) with 20 replicates per group and 1 mouse per replicate. The mice were fed by intragastric administration for 28 days. After the test, fresh fecal samples were collected and 3 samples in each group were randomly selected for investigating the microbial community and composition by using Illumina-MiSeq high-throughput sequencing technology. The results showed as follows:1) the quantities of sequences observed in DON group was less than that in control group (P>0.05). 2) The Alpha and Beta diversity were respectively reduced in DON group, and the Shannon index was also significantly decreased compared with the control group (P<0.05). 3) At the phylum level, compared with the control group, the abundances of Bacteroidetes and Deferribacteres in DON group were significantly decreased (P<0.05), while the abundance of Proteobacteria was significantly increased (P<0.05). At the genus level, compared with the control group, the abundances of Parabacteroides, Rikenella, Algoriphagus, Mucispirillum, Methylophilus and Francisella in DON group were significantly decreased (P<0.05), but the abundances of Clostridium, Robinsoniella, Allobaculum and Akkermansia in DON group were significantly increased (P<0.05). 4) The cluster analysis showed low similarity of intestinal microflora between the control and DON group. In conclusion, the bacterial diversity and species abundance of intestinal microbiota can be influenced by DON, and it is suggested that the intestinal lesions caused by consumption of DON may be related with the change of intestinal bacteria communities.

Cite this article

YANG Junhua , ZHAO Zhihui , GUO Wenbo , GUO Jinli . Effects of Deoxynivalenol on Intestinal Microbiota of Mice Analyzed by Illumina-MiSeq High-Throughput Sequencing Technology[J]. Chinese Journal of Animal Nutrition, 2017 , 29(1) : 158 -167 . DOI: 10.3969/j.issn.1006-267x.2017.01.018

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