FEED SCIENCE AND TECHNOLOGY

Effects of Dietary Betaine on Performance, Egg Quality and Serum Biochemical Parameters of Laying Hens under Heat Stress Condition

  • HAO Shengyan ,
  • LIU Longsheng ,
  • WANG Guodong ,
  • GU Xian ,
  • PAN Faming
Expand
  • Institute of Animal Husbandry, Pasture and Green Agriculture, Gansu Academy of Agricultural Science, Lanzhou 730070, China

Received date: 2016-07-12

  Online published: 2017-01-16

Abstract

This experiment was conducted to study the effects of dietary betaine on performance, egg quality and serum biochemical parameters of laying hens under heat stress condition. Six hundred 22-week-old commercial Roman laying hens were randomly divided into 5 groups with 8 replicates per group and 15 hens per replicate. The temperature humidity index (THI) of group Ⅰ (positive control group) was between 64.9 to 68.9, which was fed a basal diet in normal thermal environment; the THI of group Ⅱ (negative control group) was greater than 72, which was fed a basal diet under heat stress condition; while groups Ⅲ to Ⅴ were fed the basal diets supplemented with 200, 400 and 600 mg/kg betaine under heat stress condition, respectively, the THI of them were greater than 72 as well. The experiment lasted for 14 weeks. The results showed that there were no significant differences in average daily feed intake, feed/egg and broken egg rate among all groups (P>0.05). Compared with the group Ⅰ, the hen-housed laying rate, hen-housed egg yield, serum total protein (TP) content and serum alkaline phosphatase (AKP) activity of group Ⅱ were significantly decreased (P<0.05), but the activities of glutamic-oxalacetic transaminase (GOT), creatine kinase (CK) and glutamic-pyruvic transaminase (GPT) of group Ⅱ were significantly increased (P<0.05). Compared with the group Ⅱ, the hen-housed egg production, hen-housed egg yield and serum TP content of group Ⅳ were significantly increased (P<0.05), the hen-housed egg yield and the contents of TP and albumin (ALB) in serum of group Ⅴ were significantly increased (P<0.05), the activities of CK and GPT in serum of groups Ⅳ and Ⅴ were significantly decreased (P<0.05), the serum triglyceride (TG) content of group Ⅴ were significantly decreased (P<0.05). In conclusion, the metabolism and physiological function of layer are affected by heat stress, and which resulting in a decline in performance, but dietary betaine can improve the hen-housed egg production and hen-housed laying rate, and to improve the health of layer under heat stress. The dietary appropriate betaine level is 400 mg/kg.

Cite this article

HAO Shengyan , LIU Longsheng , WANG Guodong , GU Xian , PAN Faming . Effects of Dietary Betaine on Performance, Egg Quality and Serum Biochemical Parameters of Laying Hens under Heat Stress Condition[J]. Chinese Journal of Animal Nutrition, 2017 , 29(1) : 184 -192 . DOI: 10.3969/j.issn.1006-267x.2017.01.021

References

[1] 韩天龙,赵瑞霞,高翠英,等.养殖模式对冬季商品肉鸭舍温湿指数的影响[J].家畜生态学报,2015,36(2):80-83.
[2] 李永洙,李进,张宁波,等.热应激环境下蛋鸡肠道微生物菌群多样性[J].生态学报,2015,35(5):1601-1609.
[3] BURKHOIDER K M,THOMPSON K L,EINSTEIN M E,et al.Influence of stressors on normal intestinal microbiota,intestinal morphology,and susceptibility to Salmonella enteritidis colonization in broilers[J].Poultry Science,2008,87(9):1734-1741.  
[4] LARA L J,ROSTAGNO M H.Impact of heat stress on poultry production[J].Animals,2013,3(2):356-369.  
[5] SONG J,XIAO K,KE Y L,et al.Effect of a probiotic mixture on intestinal microflora,morphology,and barrier integrity of broilers subjected to heat stress[J].Poultry Science,2014,93(3):581-588.  
[6] KIDD M T,FERKET P R,GARLICH J D.Nutritional and osmoregulatory functions of betaine[J].World's Poultry Science Journal,1997,53(2):125-139.  
[7] PEKKINEN J,OLLI K,HUOTARI A,et al.Betaine supplementation causes increase in carnitine metabolites in the muscle and liver of mice fed a high-fat diet as studied by nontargeted LC-MS metabolomics approach[J].Molecular Nutrition & Food Research,2013,57(11):1959-1968.  
[8] 贺绍君,赵书景,李静,等.甜菜碱对热应激肉鸡生长性能、十二指肠消化酶活性及盲肠微生物区系的影响[J].动物营养学报,2014,26(12):3731-3739.
[9] HUANG Y L,YANG J,XIAO F,et al.Effects of supplemental chromium source and concentration on growth performance,carcass traits,and meat quality of broilers under heat stress conditions[J].Biological Trace Element Research,2016,170(1):216-223.  
[10] KLASING K C,ADLER K L,REMUS J C,et al.Dietary betaine increases intraepithelial lymphocytes in the duodenum of coccidia-infected chicks and increases functional properties of phagocytes[J].The Journal of Nutrition,2002,132(8):2274-2282.
[11] 鲍恩东,龚远英,HARTUNG J,等.肉鸡热应激病理损伤与应激蛋白(HSP70)相关性研究[J].中国农业科学,2004,37(2):301-305.
[12] 于纪棉.持续热应激肉鸡组织中热休克蛋白表达规律与应激性损伤机理研究[D].博士学位论文.南京:南京农业大学,2009.
[13] SHEN P N,LEI P K,LIU Y C,et al.Development of a temperature measurement system for a broiler flock with thermal imaging[J].Engineering in Agriculture,Environment and Food,2016,9(3):291-295.  
[14] WILLEMSEN H,SWENNEN Q,EVERAERT N,et al.Effects of dietary supplementation of methionine and its hydroxy analog DL-2-hydroxy-4-methylthiobutanoic acid on growth performance,plasma hormone levels,and the redox status of broiler chickens exposed to high temperatures[J].Poultry Science,2011,90(10):2311-2320.  
[15] ZHANG Z Y,JIA G Q,ZUO J J,et al.Effects of constant and cyclic heat stress on muscle metabolism and meat quality of broiler breast fillet and thigh meat[J].Poultry Science,2012,91(11):2931-2937.  
[16] SOHAIL M U,HUME M E,BYRD J A,et al.Effect of supplementation of prebiotic mannan-oligosaccharides and probiotic mixture on growth performance of broilers subjected to chronic heat stress[J].Poultry Science,2012,91(9):2235-2240.  
[17] QUINTEIRO-FILHO W M,RIBEIRO A,FERRAZ-DE-PAULA V,et al.Heat stress impairs performance parameters,induces intestinal injury,and decreases macrophage activity in broiler chickens[J].Poultry Science,2010,89(9):1905-1914.  
[18] MACK L A,FELVER-GANT J N,DENNIS R L,et al.Genetic variations alter production and behavioral responses following heat stress in 2 strains of laying hens[J].Poultry Science,2013,92(2):285-294.  
[19] LIN H,DE VOS D,DECUYPERE E,et al.Dynamic changes in parameters of redox balance after mild heat stress in aged laying hens (Gallus gallus domesticus)[J].Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2008,147(1):30-35.  
[20] AZAD M A K,KIKUSATO M,MAEKAWA T,et al.Metabolic characteristics and oxidative damage to skeletal muscle in broiler chickens exposed to chronic heat stress[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2010,155(3):401-406.  
[21] EKLUND M,BAUER E,WAMATU J,et al.Potential nutritional and physiological functions of betaine in livestock[J].Nutrition Research Reviews,2005,18(1):31-48.  
[22] RIMOLDI S,LASAGNA E,SARTI F M,et al.Expression profile of six stress-related genes and productive performances of fast and slow growing broiler strains reared under heat stress conditions[J].Meta Gene,2015,6:17-25.
[23] LIPINSKI K,SZRAMKO E,JEROCH H,et al.Effects of betaine on energy utilization in growing pigs-a review[J].Annals of Animal Science,2012,12(3):291-300.
[24] EBEID T A,SUZUKI T,SUGIYAMA T.High ambient temperature influences eggshell quality and calbindin-D28k localization of eggshell gland and all intestinal segments of laying hens[J].Poultry Science,2012,91(9):2282-2287.  
[25] LIN H,MERTENS K,KEMPS B,et al.New approach of testing the effect of heat stress on eggshell quality:mechanical and material properties of eggshell and membrane[J].British Poultry Science,2004,45(4):476-482.  
[26] CABEZÓN F A,STEWART K R,SCHINCKEL A P,et al.Effect of natural betaine on estimates of semen quality in mature AI boars during summer heat stress[J].Animal Reproduction Science,2016,170:25-37.
[27] VINOTH A,THIRUNALASUNDARI T,THARIAN J A,et al.Effect of thermal manipulation during embryogenesis on liver heat shock protein expression in chronic heat stressed colored broiler chickens[J].Journal of Thermal Biology,2015,53:162-171.
[28] 董淑丽,邓雯,雷雪芹,等.热应激对动物理化特性及生产性能的影响[J].河南科技大学学报:农学版,2003,23(1):59-62,66.
[29] EGBUNIWE I C,AYO J O,KAWU M U,et al.Effects of betaine and ascorbic acid on tonic immobility,superoxide dismutase and glutathione peroxidase in broiler chickens during the hot-dry season[J].Journal of Veterinary Behavior,2016,12:60-65.
[30] HASSAN R A,EBEUD T A,ABDEl-LATEIF A I,et al.Effect of dietary betaine supplementation on growth,carcass and immunity of New Zealand White rabbits under high ambient temperature[J].Livestock Science,2011,135(2/3):103-109
[31] 陈力,王丽君,谭耀宗,等.甜菜碱对脂肪变性HepG2细胞Hcy、SAM/SAH及脂代谢相关基因mRNA表达的影响[J].华南预防医学,2013,39(3):1-6.
[32] SU S Y,DODSON M V,LI X B,et al.The effects of dietary betaine supplementation on fatty liver performance,serum parameters,histological changes,methylation status and the mRNA expression level of Spot14α in Landes goose fatty liver[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2009,154(3):308-314.  
Outlines

/