SHORT COMMUNICATIONS

Substitution of Dietary Fish Oil with Soybean Oil Influences Stress Indexes and Tissue Antioxidant Function of Silver Pomfret (Pampus argenteus) before and after Transportation

  • ZHANG Chenjie ,
  • GAO Quanxin ,
  • PENG Shiming ,
  • SHI Zhaohong ,
  • WANG Jiangang
Expand
  • East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China

Received date: 2016-07-15

  Online published: 2017-01-16

Abstract

Through detecting the changes of serum cortisol, glucose, lactate concentrations and tissue antioxidant indexes, the influences of substitution of dietary fish oil with soybean oil on stress indexes and tissue antioxidant function of silver pomfret (Pampus argenteus) before and after Transportation were studied. Four experimental diets were designed, which used 100% fish oil (FO group), 70% fish oil+30% soybean oil (FSO group), 30% fish oil+70% soybean oil (SFO group) and 100% soybean oil (SO) as the fat source, respectively. The silver pomfret with the average body weight of (17.2±6.7) g had been fed with these experimental diets for 60 days, and then they were being transported for 4 h. Each diet fed 3 tanks (replicates) with 50 fish per tank. The results showed as follows:after 4 h transportation stress, serum cortisol concentration of each group was increased, and the change of SO group was significant that up-regulated to 1.89 folds (P<0.05), but the change of SFO group was lighter than other groups; serum glucose concentration of each group increased slightly, the change of each group was not significant (P>0.05), and the SFO group was the least one; then, serum lactate concentration of each group increased significantly (P<0.05), especially SO group. Brain acetyl-cholinesterase (AChE) activity of each group decreased significantly, and SFO was the highest group both before and after transportation; serum superoxide dismutase (SOD) activity of every group increased significantly (P<0.05), especially SO group; serum catalase (CAT) activity increased in all groups, but only SO group increased significantly (P<0.05); CAT activity in liver and muscle of SO group increased significantly (P<0.05), and the changes of other groups were not significant (P>0.05); serum SOD and CAT activities of SO group increased significantly (P<0.05). The results indicate that the health of silver pomfret in group SO is worse than other groups after transporting, and its stress response is more significant. It is suggested that the substitution of dietary fish oil with too much soybean oil is disadvantageous to transportation and health of silver pomfret. And it is important to keep the proper proportion of fish oil and soybean oil in the diet.

Cite this article

ZHANG Chenjie , GAO Quanxin , PENG Shiming , SHI Zhaohong , WANG Jiangang . Substitution of Dietary Fish Oil with Soybean Oil Influences Stress Indexes and Tissue Antioxidant Function of Silver Pomfret (Pampus argenteus) before and after Transportation[J]. Chinese Journal of Animal Nutrition, 2017 , 29(1) : 354 -364 . DOI: 10.3969/j.issn.1006-267x.2017.01.041

References

[1] 刘静,李春生,李显森.中国鲳属鱼类的分类研究[J].海洋科学集刊,2002(44):240-252.
[2] 孙鹏飞,戴芳群,陈云龙,等.长江口及其邻近海域渔业资源结构的季节变化[J].渔业科学进展,2015,36(6):8-16.
[3] 施兆鸿,彭士明,王建钢,等.人工养殖银鲳子代的胚胎发育及仔稚幼鱼形态观察[J].中国水产科学,2011,18(2):267-274.
[4] 彭士明,施兆鸿,孙鹏,等.养殖密度对银鲳幼鱼生长及组织生化指标的影响[J].生态学杂志,2010,29(7):1371-1376.
[5] XIE D,WANG S,YOU C,et al.Characteristics of LC-PUFA biosynthesis in marine herbivorous teleost Siganus canaliculatus under different ambient salinities[J].Aquaculture Nutrition,2015,21(5):541-551.  
[6] TURCHINI G M,TORSTENSEN B E,NG W K.Fish oil replacement in finfish nutrition[J].Reviews in Aquaculture,2009,1(1):10-57.  
[7] 蒋湘辉,骆小年,金广海,等.不同蛋白质和脂肪水平饲料对拉氏鱼岁幼鱼生长、体成分和免疫力的影响[J].动物营养学报,2016,28(3):864-871.
[8] VILLASANTE A,PATRO B,CHEW B,et al.Dietary intake of purple corn extract reduces fat body content and improves antioxidant capacity and n-3 polyunsaturated fatty acid profile in plasma of rainbow trout,Oncorhynchus mykiss[J].Journal of the World Aquaculture Society,2015,46(4):381-394.  
[9] 田晶晶,雷彩霞,吉红.饲料亚油酸/亚麻酸比率对松浦镜鲤生长和健康状况的影响[J].淡水渔业,2015,45(5):76-82.
[10] URBINATI E C,ABREU J S D,CAMARGO A C D S,et al.Loading and transport stress of juvenile matrinxã (Brycon cephalus,Characidae) at various densities[J].Aquaculture,2004,229(1/2/3/4):389-400.
[11] 王文博,李爱华.环境胁迫对鱼类免疫系统影响的研究概况[J].水产学报,2002,26(4):368-374.
[12] KING H R.Fish transport in the aquaculture sector:an overview of the road transport of Atlantic salmon in Tasmania[J].Journal of Veterinary Behavior,2009,4(4):163-168.  
[13] PAN C H,CHIEN Y H,WANG Y J.The antioxidant capacity response to hypoxia stress during transportation of characins (Hyphessobrycon callistus Boulenger) fed diets supplemented with carotenoids[J].Aquaculture Research,2010,41(7):973-981.
[14] PARODI T V,CUNHA M A,BECKER A G,et al.Anesthetic activity of the essential oil of Aloysia triphylla and effectiveness in reducing stress during transport of albino and gray strains of silver catfish,Rhamdia quelen[J].Fish Physiology and Biochemistry,2014,40(2):323-334.  
[15] AZAMBUJA C R,MATTIAZZI J,RIFFEL A P,et al.Effect of the essential oil of Lippia alba on oxidative stress parameters in silver catfish (Rhamdia quelen) subjected to transport[J].Aquaculture,2011,319(1/2):156-161.
[16] DHANASIRI A K S,FERNANDES J M O,KIRON V.Glutamine synthetase activity and the expression of three glul paralogues in zebrafish during transport[J].Comparative Biochemistry and Physiology Part B,2012,163(3/4):274-284.
[17] 杜浩,危起伟,甘芳,等.苯唑卡因对美洲鲥运输应激的缓解作用研究[J].中国水产科学,2006,13(5):787-793.
[18] 杜浩,危起伟,甘芳,等.美洲鲥应激后皮质醇激素和血液生化指标的变化[J].动物学杂志,2006,41(3):80-84.
[19] TACCHI L,LOWREY L,MUSHARRAFIEH R,et al.Effects of transportation stress and addition of salt to transport water on the skin mucosal homeostasis of rainbow trout (Oncorhynchus mykiss)[J].Aquaculture,2015,435:120-127.
[20] 张伟,王有基,李伟明,等.运输密度和盐度对大黄鱼幼鱼皮质醇、糖元及乳酸含量的影响[J].水产学报,2014,38(7):973-980.
[21] MANUEL R,BOERRIGTER J,ROQUES J,et al.Stress in African catfish (Clarias gariepinus) following overland transportation[J].Fish Physiology and Biochemistry,2014,40(1):33-44.  
[22] BOERRIGTER J G J,MANUEL R,VAN DEN BOS R,et al.Recovery from transportation by road of farmed European eel (Anguilla anguilla)[J].Aquaculture Research,2015,46(5):1248-1260.  
[23] OYOO-OKOTH E,CHEROP L,NGUGI C C,et al.Survival and physiological response of Labeo victorianus (Pisces:Cyprinidae,Boulenger 1901) juveniles to transport stress under a salinity gradient[J].Aquaculture,2011,319(1/2):226-231.
[24] 徐钢春,杜富宽,聂志娟,等.10‰盐度对长江刀鲚幼鱼装载和运输胁迫中应激指标的影响[J].水生生物学报,2015,39(1):66-72.
[25] 胡培培,刘汝鹏,赵忠波,等.运输时间和密度对翘嘴鲌皮质醇、耗氧率及氧气袋内水质的影响[J].水生生物学报,2014,38(6):1190-1194.
[26] IVERSEN M,FINSTAD B,MCKINLEY R S,et al.Stress responses in Atlantic salmon (Salmo salar L.) smolts during commercial well boat transports,and effects on survival after transfer to sea[J].Aquaculture,2005,243(1/2/3/4):373-382.
[27] 彭士明,施兆鸿,李杰,等.运输胁迫对银鲳血清皮质醇、血糖、组织中糖元及乳酸含量的影响[J].水产学报,2011,35(6):831-837.
[28] PENG S M,SHI Z H,FEI Y,et al.Effect of high-dose vitamin C supplementation on growth,tissue ascorbic acid concentrations and physiological response to transportation stress in juvenile silver pomfret,Pampus argenteus[J].Journal of Applied Ichthyology,2013,29(6):1337-1341.  
[29] MOLDAL T,LØKKA G,WIIK-NIELSEN J,et al.Substitution of dietary fish oil with plant oils is associated with shortened mid intestinal folds in Atlantic salmon (Salmo salar)[J].BMC Veterinary Research,2014,10:60.
[30] BECKER A G,PARODI T V,GONÇALVES J F,et al.Ectonucleotidase and acetylcholinesterase activities in silver catfish (Rhamdia quelen) exposed to different salinities[J].Biochemical Systematics and Ecology,2013,46:44-49.
[31] ASSIS C R D D,LINHARES A G,OLIVEIRA V M,et al.Characterization of catalytic efficiency parameters of brain cholinesterases in tropical fish[J].Fish Physiology and Biochemistry,2014,40(6):1659-1668.  
[32] MARTÍNEZ-ÁLVAREZ R M,MORALES A E,SANZ A.Antioxidant defenses in fish:biotic and abiotic factors[J].Reviews in Fish Biology and Fisheries,2005,15(1/2):75-88.
[33] 曾本和,廖增艳,吴双,等.饲料脂肪水平对大鳞副泥鳅幼鱼生长性能、消化酶活性及抗氧化能力的影响[J].动物营养学报,2016,28(4):1105-1113.
[34] KANAK E G,DOGAN Z,EROGLU A,et al.Effects of fish size on the response of antioxidant systems of Oreochromis niloticus following metal exposures[J].Fish Physiology and Biochemistry,2014,40(4):1083-1091.
[35] 孙鹏,柴学军,尹飞,等.运输胁迫下日本黄姑鱼肝脏抗氧化系统的响应[J].海洋渔业,2014,36(5):469-474.
[36] 高金伟,杜富宽,顾若波,等.运输应激对刀鲚生理生化指标和HPI轴基因表达影响及甘草甜素的作用[J].上海海洋大学学报,2015,24(6):817-825.
[37] MOZANZADEH M T,MARAMMAZI J G,YAVARI V,et al.Dietary n-3 LC-PUFA requirements in silvery-black porgy juveniles (Sparidentex hasta)[J].Aquaculture,2015,448:151-161.
Outlines

/