Effects of Dietary Nutrition Restrictions on Intestinal Morphology and Serum Insulin-Like Growth Factor-1 and Glucagon-Like Peptide-2 Concentrations of Lambs

QI Minli, DIAO Qiyu, MA Tiewei, CHAI Jianmin, WANG Bo, CUI Kai, WANG Jie, ZHANG Naifeng

doi:10.3969/j.issn.1006-267x.2017.02.009

2017 , Vol. 29 >Issue 2: 426 - 435

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.02.009

Molecular Nutrition

Effects of Dietary Nutrition Restrictions on Intestinal Morphology and Serum Insulin-Like Growth Factor-1 and Glucagon-Like Peptide-2 Concentrations of Lambs

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1. Feed Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Feed Biotechnology of the Ministry of Agriculture, Beijing 100081, China;
2. Nanjing Agricultural University, Nanjing 210000, China

Received date: 2016-07-01

Online published: 2017-02-17

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Abstract

The aim of this study was to assess the effects of dietary nutrition restrictions on intestinal morphology and serum insulin-like growth factor-1 (IGF-1) and glucagon-like peptide-2 (GLP-2) concentrations of lambs. Sixty-four 17-day-old Hu lambs were randomly divided into four groups with four replicates per group and four lambs per replicate (half male and half female), including control (CON) group, 20% of protein restriction (PR group), 20% of energy restriction (ER group), and 20% of energy+20% of protein restriction (BR group). Serum sample was collected at 20, 40 and 60 days of age to determine serum concentrations of GLP-2 and IGF-1. Four lambs of each group were slaughtered at 40 and 60 days of age, respectively, to observer morphological structure of duodenum, jejunum and ileum. The results showed as follows:1) the weight of small intestine was significantly reduced in PR, ER and BR groups compared with CON group at 40 days of age (P<0.05), and was significantly reduced only in ER group compared with CON group at 60 days of age(P<0.05). 2) Villus height of duodenum and jejunum at 40 days of age, and of duodenum at 60 days of age in PR, ER and BR groups was significantly lower than that of CON group (P<0.05), villus height of jejunum at 60 days of age in ER and BR groups was significantly lower than that of CON group (P<0.05). 3) Villus height/crypt depth (V/C) of duodenum at 40 days of age in BR group was significantly reduced compared with CON group (P<0.05). 4) Serum concentration of GLP-2 was significantly reduced in ER group compared with the other groups at 60 days of age (P<0.05), but serum IGF-1 concentration was not significantly differed in 4 groups (P>0.05). In conclusion, dietary nutrition restrictions reduce morphological development of duodenum and jejunum, and energy restriction reduces serum concentration of GLP-2 in lambs.

Key words： lamb; nutrition restriction; morphology; insulin-like growth factor-1; glucagon-like peptide-2

Cite this article

QI Minli, DIAO Qiyu, MA Tiewei, CHAI Jianmin, WANG Bo, CUI Kai, WANG Jie, ZHANG Naifeng . Effects of Dietary Nutrition Restrictions on Intestinal Morphology and Serum Insulin-Like Growth Factor-1 and Glucagon-Like Peptide-2 Concentrations of Lambs[J]. Chinese Journal of Animal Nutrition, 2017 , 29(2) : 426 -435 . DOI: 10.3969/j.issn.1006-267x.2017.02.009

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