This paper was aimed to study the effects of glucose oxidase (GOD) on nutrient utilization, cecal microflora structure and intestinal digestive enzyme activities of growing Rex rabbits. A total of 150 growing Rex rabbits with the age of 40 days were randomly assigned to 5 groups with 5 replicates per group and 6 rabbits per replicate (half male and half female). The rabbits in control group were fed a basal diet, while the others in 4 experimental groups were fed the basal diet supplemented with 0.1%, 0.2%, 0.3% and 0.4% GOD, respectively. The trial lasted for 5 days for adaptation and 30 days for test. The results showed as follows:1) the apparent digestibility of energy and apparent digestible energy reached to the minimum in the control group, while reached to the maximum in the 0.4% group. Compared with the control group, crude fibre apparent digestibility of experimental groups were elevated more or less, and 0.4% group was significantly increased by 16.10% (P<0.05). 2) Compared with the control group, the digestible nitrogen, nitrogen retention, nitrogen utilization and nitrogen bioavailability of 0.4% group were significantly increased by 12.39%, 17.48%, 15.68% and 12.78%, respectively (P<0.05). 3) GOD addition could decrease the pH in caecum to a certain degree. Compared with the control group, the amounts of Lactobacilli and Bifidobacterium in caecum of 0.4% group were significantly increased (P<0.05), while the amount of Escherichia coli was significantly reduced (P<0.05); moreover, the amount of Bifidobacterium in caecum of 0.3% group was significantly increased (P<0.05). 4) Compared with the control group, the trypsin activity in duodenum of 0.4% group was significantly increased (P<0.05), and the lactase activity in jejunum was significantly increased (P<0.01). By synthesize analyses, diets with GOD can improve the intestinal microflora structure, digestive enzyme activities and nutrient utilization of Rex rabbits. Addition of 0.4% (60 U/kg) GOD is recommended.