Molecular Nutrition

Effects of Arachidonic Acid on Lipid Metabolism-Related Gene Expressions of Hepatopancreas Cells Isolated from Juvenile Oriental River Prawn, Macrobrachium nipponense

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  • 1. Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition, Chinese Academy of Fishery Sciences, College of Life Sciences, Huzhou University, Huzhou 313000, China;
    2. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116000, China

Received date: 2016-08-06

  Online published: 2017-02-17

Abstract

This experiment was conducted to determine the effects of arachidonic acid (ARA) concentration in culture medium on cell viability and lipid metabolism-related gene expressions of hepatopancreas cells isolated from juvenile oriental river prawn, Macrobrachium nipponense. The hepatopancreas cells were dissected from prawns and were cultured with complete culture medium for 5 days. After that, cultured cells were incubated in medium supplemented with graded levels[0 (ARA1), 50 (ARA2), 100 (ARA3), 200 (ARA4) and 1 000 μmol/L (ARA5) of ARA. Cell viability at 24 h and gene expressions of lipid metabolism-related genes at 12 and 24 h were examined. The results showed as follows:the hepatopancreas cells showed well growth in complete culture medium, and could survive for 15 days; cell viability was significantly decreased by incubation with higher level (1 000 μmol/L) of ARA (ARA5 group) compared with ARA1 and ARA2 groups (P<0.05) after 24 h; higher level (1 000 μmol/L) of ARA (ARA5 group) caused significant decreases of gene expressions of delta-4 fatty acyl desaturase (Δ4 FAD), delta-6 fatty acyl desaturase (Δ6 FAD), very-long-chain fatty acids-6 (Elovl6), scavenger receptor class B type Ⅰ (SR-BⅠ), fatty acid-binding protein 10 (FABP10) and acyl-CoA binding protein (ACBP) of hepatopancreas cells incubation for both 12 and 24 h; after incubation with ARA for 12 h, the gene expression of SR-BⅠ of ARA2 group was significantly higher than that of other groups (P<0.05), FABP10 gene expression of ARA2 and ARA3 groups was significantly higher than that of ARA1 and ARA5 groups (P<0.05), and ACBP gene expression of ARA3 group was significantly higher than that of other groups (P<0.05); after incubation with ARA for 24 h, the highest expressions of SR-BⅠ, FABP10 and ACBP were observed in ARA2 group, which was significantly higher than those of other groups (P<0.05). These findings suggest that ARA can influence cell viability and lipid metabolism-related gene expressions of hepatopancreas cell isolated from Macrobrachium nipponense. Cell viability can be decreased by incubation with higher level of ARA (1 000 μmol/L). Appropriate levels of ARA (50 to 100 μmol/L) can promote the expressions of genes related to fatty acyl desaturase, elongases of very-long-chain fatty acids and fatty acid transport.

Cite this article

DING Zhili, CAO Fang, LUO Na, KONG Youqin, ZHANG Yixiang, LI Jingfen, YE Jinyun . Effects of Arachidonic Acid on Lipid Metabolism-Related Gene Expressions of Hepatopancreas Cells Isolated from Juvenile Oriental River Prawn, Macrobrachium nipponense[J]. Chinese Journal of Animal Nutrition, 2017 , 29(2) : 536 -546 . DOI: 10.3969/j.issn.1006-267x.2017.02.021

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