Molecular Nutrition

Ontogenetic Regulation of Glucose Transporter and Insulin Receptor mRNA Expression in Skeletal Muscle of Different Kinds of Pigs at Different Physiological Stages

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  • College of Animal Science, South China Agricultural University, Guangzhou 510642, China

Received date: 2016-07-28

  Online published: 2017-02-17

Abstract

This experiment was conducted to determine the ontogenetic regulation of glucose transporter (GLUT) 1, GLUT4 and insulin receptor (IR) mRNA expression in skeletal muscle of different kinds of pigs at different physiological stages. Lantang (LT) and Landrace (LD) pigs at the age of 1, 7, 30, 60, 90 and 150 days were selected to determine the expression patterns of GLUT1 and GLUT4 at different skeletal muscle tissues including longissimus dorsi muscles (LM), semimembranosus muscles (SM) and semitendinosus muscles (SD) by the method of real-time semiquantitative PCR for cDNA templates. The results showed as follows:1) LT and LD pigs exhibited similar development of mRNA expressions of GLUT1 in the LM, SM and SD from 1 to 150 days of age with the highest level at the age of 1 day. 2) LT and LD pigs exhibited waveform variations of mRNA relative expression abundance of GLUT4 from 1 to 150 days of age with breed differences in the speed and extend of variations. At the age of 1 and 150 days, the mRNA relative expression abundance of GLUT4 in the LM of LT pig was lower than that of LD pig with the same age (P>0.05), while was higher than that of LD pig with the same age of the rest days (P>0.05). 3) LT and LD pigs exhibited higher level of the mRNA relative expression abundance of IR in the LM, SM and SD at the age of 1 and 30 days, while there were not significant difference among 7, 60, 90 and 150 days of age (P>0.05). There was no significant difference in mRNA relative expression abundance of IR between LT and LD pigs with the same ages (P>0.05). The results suggest that GLUT1 mRNA expression in skeletal muscles of pigs can be regulated by physiological stages, while GLUT4 mRNA expression is not only differentially regulated by breeds but also differentially distributed in physiological stages, and the expression level of GLUT4 is weakly related to the IR mRNA expression and shows no apparent rules.

Cite this article

LIU Wenzuo, ZUO Jianjun, DAI Fawen, YE Hui, CAO Qingyun, ZHOU Xiangyan, ZOU Shigeng, HUANG Zhiyi, ZHI Aimin, FENG Dingyuan . Ontogenetic Regulation of Glucose Transporter and Insulin Receptor mRNA Expression in Skeletal Muscle of Different Kinds of Pigs at Different Physiological Stages[J]. Chinese Journal of Animal Nutrition, 2017 , 29(2) : 605 -612 . DOI: 10.3969/j.issn.1006-267x.2017.02.029

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