Effects of Different Nitrogen/Energy Diets on Expression of Genes Related to Umbilical Vessel Development of Pregnant Huanjiang Mini-Pigs

ZHU Qian, MA Sicong, JI Yujiao, YIN Yulong, KONG Xiangfeng

doi:10.3969/j.issn.1006-267x.2017.02.030

2017 , Vol. 29 >Issue 2: 613 - 619

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.02.030

Molecular Nutrition

Effects of Different Nitrogen/Energy Diets on Expression of Genes Related to Umbilical Vessel Development of Pregnant Huanjiang Mini-Pigs

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1. Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Center of Healthy Livestock, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
2. Research Center of Mini-Pig, Huanjiang Observation and Research Station for Karst Ecosystems, Chinese Academy of Sciences, Huanjiang 547100, China

Received date: 2016-08-01

Online published: 2017-02-17

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Abstract

This study was conducted to investigate the effects of different nitrogen/energy diets on expression of genes related to umbilical vessel development of pregnant Huanjiang mini-pigs. A total of 48 primiparous Huanjiang mini-pigs were randomly assigned into two groups according to the body weight (BW), and reared in eight replicates (pens) with three mini-pigs per replicate. After service, the animals were fed with high nitrogen/energy diet (digestible energy was 14.73 MJ/kg, crude protein content was 13.11%, nitrogen/energy was 0.89) and low nitrogen/energy diet (digestible energy was 12.24 MJ/kg, crude protein content was 9.77%, nitrogen/energy was 0.80), respectively. At 45, 75 and 110 d of gestation, one pig was randomly selected and sacrificed from each pen. The umbilical cord tissues were collected from three fetuses with maximal BW, average BW and minimal BW per litter. The expression levels of fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor 2 (VEGFR2) and endothelial nitric oxide synthase (eNOS), which related to umbilical vessel development, were detected by RT-qPCR technique. The results showed that compared with the low nitrogen/energy diet group, the high nitrogen/energy diet significantly increased the expression levels of genes related to umbilical vessel development (P<0.05), including VEGF and VEGFR2 genes expression levels of fetus at 45 d of gestation with maximal BW; VEGF gene expression level of fetus at 45 d of gestation with average BW; VEGF, VEGFR2 and eNOS genes expression levels of fetus at 75 d of gestation with maximal BW; VEGF and eNOS genes expression levels of fetus at 75 d of gestation with average BW, VEGFR2 gene expression level of fetus at 75 d of gestation with minimal BW; FGF, VEGFR2 and eNOS genes expression levels of fetus at 110 d of gestation with maximal BW, FGF and VEGFR2 genes expression levels of fetus at 110 d of gestation with average BW, and VEGF and eNOS genes expression levels of fetus at 110 d of gestation with minimal BW. The expression levels of umbilical vessel genes of fetuses in different gestation stages with the same standard BW were different, as well as of fetuses with different standard BW in a same gestation stage. These findings suggest that the high nitrogen/energy diet containing higher levels of protein and amino acids can significantly increase the expression levels of genes related to the umbilical vessel development in pregnant Huanjiang mini-pigs, and the tested gene expression levels are different during different gestation stages, as well as among the fetuses with different standard body weights during a same gestation stage.

Key words： Huanjiang mini-pigs; pregnancy; dietary nitrogen/energy ratio; umbilical cord; blood vessel development

Cite this article

ZHU Qian, MA Sicong, JI Yujiao, YIN Yulong, KONG Xiangfeng . Effects of Different Nitrogen/Energy Diets on Expression of Genes Related to Umbilical Vessel Development of Pregnant Huanjiang Mini-Pigs[J]. Chinese Journal of Animal Nutrition, 2017 , 29(2) : 613 -619 . DOI: 10.3969/j.issn.1006-267x.2017.02.030

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