Molecular Nutrition

Effects of Tea Saponin on Rumen Protozoa Flora of Dairy Cows

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  • 1. Key Laboratory for Dairy Cow Nutrition of Beijing, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China;
    2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2016-08-01

  Online published: 2017-02-17

Abstract

The objective of this study was to research the effects of tea saponin on rumen protozoa flora of dairy cows. Twelve healthy Holstein dairy cows[body weight was (550±30) kg; milk yield was 35 kg/(head·d); 2 to 4 parities] were randomly allocated to one of four groups with 3 cows per group. Tea saponin[0 (control), 20, 30 and 40 g/(head·d)] was perfused to cows before morning feeding. The experiment lasted for 49 d with 14 d of adaptation period and 35 d of formal test period. Rumen fluid was collected by mouth sampler at 1 h before morning feeding every 7 d during formal test period. Denaturing gradient gel electrophoresis (DGGE) combined with 18S rDNA sequence analysis technique was used for rumen protozoa flora analysis. The results showed as follows:1) tea saponin could selectively inhibit protozoa growth. 2) Compared with control group, richness index and Shannon index in 30 and 40 g/(head·d) tea saponin groups were significantly reduced (P<0.05), and dominance index was significantly increased (P<0.05), but evenness index was not significantly different among groups (P>0.05); tea saponin could significantly reduce rumen Vestibuliferia Entodiniomorphida protozoa number. In conclusion, dietary supplementation of 30 and 40 g/(head·d) tea saponin can inhibit protozoa growth in rumen of dairy cows, and reduce the diversity of rumen protozoa.

Cite this article

ZHAO Shiping, YAN Shuhong, JIANG Qihui, CHANG Xiaoxiao, WANG Bing, FANG Luoyun, JIANG Linshu, XIONG Benhai . Effects of Tea Saponin on Rumen Protozoa Flora of Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2017 , 29(2) : 620 -627 . DOI: 10.3969/j.issn.1006-267x.2017.02.031

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