Molecular Nutrition

Effects of Nutritional Restriction during Late Gestation on Gene Expressions of Glucose Transporters in Gastrointestinal Tract of Ewes

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  • 1. Key Laboratory for Agro-Ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, South-Central Experimental Station of Animal Nutrition and Feed Science in Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
    2. Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Received date: 2016-08-24

  Online published: 2017-02-17

Abstract

This experiment was conducted to investigate the effects of nutritional restriction during late gestation on gene expressions of glucose transporters in gastrointestinal tract of ewes. Twenty Xiangdong black pregnant goats were chose and randomly assigned to 2 groups:control group (ad libtum) and restricted group (40% restriction of feed intake), and each group had 10 ewes. The pre-trial lasted for 15 d (81 to 95 d of gestation), and the trial lasted for 39 d (96 to 135 d of gestation). After the trial, ewes were slaughtered to collected mucosa samples of rumen, duodenum, jejunum, ileum and cecum. Real-time PCR analysis was used to determine the gene expression levels of sodium/glucose cotransporter member 1 (SGLT1), sodium/glucose cotransporter member 3 (SGLT3), facilitated glucose transporter member 2 (GLUT2) and facilitated glucose transporter member 5 (GLUT5). The results showed as follows:compared with control group, the expression level of SGLT1 gene in rumen of restricted group was significantly decreased (P<0.05), and there was a decreased tendency for that in jejunum and ileum (0.05≤P < 0.10). The expression level of GLUT5 gene was significantly decreased in cecum (P<0.05); while the expression levels of other glucose transporter genes in gastrointestinal tracts were not significantly different between restricted group and control group (P>0.05). Collectively, nutritional restriction has different effects on the expressions of glucose transporter genes located on gastrointestinal tracts, thereby leading to the changes of glucose transport.

Cite this article

WU Jian, LI Xiaopeng, HE Zhixiong, JIAO Jinzhen, TAN Zhiliang . Effects of Nutritional Restriction during Late Gestation on Gene Expressions of Glucose Transporters in Gastrointestinal Tract of Ewes[J]. Chinese Journal of Animal Nutrition, 2017 , 29(2) : 645 -651 . DOI: 10.3969/j.issn.1006-267x.2017.02.034

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